A5101423569- SARS-CoV-2 and COVID-19 Research
- Force Microscopy Techniques and Applications
- SARS-CoV-2 detection and testing
- Metal Forming Simulation Techniques
- Growth and nutrition in plants
- Protein Structure and Dynamics
- Enzyme Structure and Function
- COVID-19 Clinical Research Studies
- Clay minerals and soil interactions
- Iron oxide chemistry and applications
- Advanced Materials and Mechanics
- Animal Virus Infections Studies
- Pineapple and bromelain studies
- Advanced Electron Microscopy Techniques and Applications
- Vector-Borne Animal Diseases
- Heavy metals in environment
- Heat shock proteins research
- Molecular Junctions and Nanostructures
- Biochemical and Structural Characterization
- Orbital Angular Momentum in Optics
- T-cell and Retrovirus Studies
- Plant Physiology and Cultivation Studies
- Banana Cultivation and Research
- Berry genetics and cultivation research
University of Chile
1997-2025
ABSTRACT Background The newly described SARS-CoV-2 lineage C.37 was recently classified as a variant of interest by the WHO (Lambda variant) based on its high circulation rates in South American countries and presence critical mutations spike protein. impact such infectivity immune escape from neutralizing antibodies are entirely unknown. Methods We performed pseudotyped virus neutralization assay determined Lambda using plasma samples healthcare workers (HCW) two centers Santiago, Chile who...
Abstract Knots are natural topologies of chains. Yet, little is known about spontaneous knot formation in a polypeptide chain—an event that can potentially impair its folding—and the effect on stability and folding kinetics protein. Here we used optical tweezers to show free energy cost form trefoil denatured state chain 120 residues 5.8 ± 1 kcal mol −1 . Monte Carlo dynamics random chains predict this value, indicating entropic origin. This predicted remain above 3 for proteins as large 900...
BiP (Immunoglobulin Binding Protein) is a member of the Hsp70 chaperones that participates in protein folding endoplasmic reticulum. The function relies on cycles ATP hydrolysis driving binding and release its substrate proteins. It still remains unknown how affects pathway there has been no direct demonstration showing which state bound by BiP, as previous work used only peptides. Here, we employ optical tweezers for single molecule force spectroscopy experiments to investigate mechanism...
Human T-lymphotropic virus-type 1 (HTLV-1) is the etiologic agent of neurologic disease HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Tax viral protein plays a critical role in pathogenesis. Previous studies suggested that extracellular might involve cytokine-like effects. We evaluated secretion 18 h-ex vivo peripheral blood mononuclear cells (PBMCs) cultures from 15 HAM/TSP patients and asymptomatic carriers. Futhermore, plasma level was other 12 10 Proviral load mRNA...
Abstract Zinc (Zn) speciation was determined in a Zn pre‐treated Andosol, Inceptisol, and Vertisol before after adding phosphate. The fractions obtained, i.e., soluble, exchangeable, iron‐manganese (Fe‐Mn) bound, chelated insoluble organic forms had different distribution the three soils. exchangeable form predominated followed by Fe‐Mn oxide bound minor amounts of forms, whereas very abundant Inceptisol Vertisol. Sizable were also present. Phosphate pre‐treatment did not alter Zn, Andosol...
Protein folding occurs because establishment of weak interactions observed in the native state (native contacts) predominate over formation transient that could promote alternative conformations (non‐native contacts). However, knotted proteins defy this view their polypeptide chains would require to form non‐native contacts during its folding. To determine role such topologies, we explored mechanism an artificial protein (2ouf‐knot). In case, optimization should play a minor The 2ouf‐knot...
Abstract Here, we used pseudotyped viruses to characterize the neutralization capacity of antibodies elicited by CoronaVac and BNT162b2 vaccines against emerging variant interest Lambda. We observed that elicits higher neutralizing antibody titers than CoronaVac, ranging from 5.8-fold for ancestral spike 9.4-fold Lambda variant. Neutralization D614G, Alpha, Gamma, variants was reduced between 1.78 3.05-fold 1.10 1.87-fold BNT162b2. Structural analyses show significant changes in antigenic...
Knotted polypeptides constitute a group of proteins whose backbone chain entangles in the folded state. The folding mechanism knotted protein is complex, involving multiple intermediate states. Therefore, it difficult to determine experimentally thermodynamic and kinetic barriers associated with threading polypeptide chain. To address this problem, we used optical tweezers study MJ0366, smallest containing shallow trefoil knot. Specifically, mechanically manipulated from different pulling...