A5049228890- RNA Research and Splicing
- RNA modifications and cancer
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Vaccine Coverage and Hesitancy
- COVID-19 Pandemic Impacts
- HIV/AIDS drug development and treatment
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
University of Chile
2016-2022
Universidad Autónoma de Chile
2021-2022
During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, mechanisms by which HIV-1 Gag protein selects two molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate deposition of N6-methyladenosine (m6A) regulates packaging. While m6A METTL3/METTL14 onto was associated with increased synthesis reduced packaging, FTO-mediated demethylation promoted incorporation viral particles. Interestingly, associates...
Control of the COVID-19 pandemic largely depends on effectiveness vaccination process. An understanding factors that underlie willingness to accept contributes pivotal information controlling pandemic. We analyzed association between available vaccines and vaccine determinants amidst Chilean Individual-level survey data was collected from 744 nationally representative respondents multivariate regression models were used estimate outcome explanatory variables. found trust in vaccines,...
Gag synthesis from the full-length unspliced mRNA is critical for production of viral progeny during human immunodeficiency virus type-1 (HIV-1) replication. While most spliced mRNAs follow canonical gene expression pathway in which recruitment nuclear cap-binding complex (CBC) and exon junction (EJC) largely stimulates rates export translation, relies on protein Rev to reach cytoplasm recruit host translational machinery. Here, we confirm that ensures high levels by driving translation...
Translation initiation of the human immunodeficiency virus type-1 (HIV-1) full-length RNA has been shown to occur through cap-dependent and IRES-driven mechanisms. Previous studies suggested that nuclear cap-binding complex (CBC) rather than eIF4E drives translation we have recently reported CBC subunit CBP80 supports function viral protein Rev during export this transcript. Ribosome recruitment CBC-dependent cellular mRNAs relies on activity CBP80/20 factor (CTIF), which bridges 40S...
Abstract Gag synthesis from the full-length unspliced mRNA is critical for production of viral progeny during human immunodeficiency virus type-1 (HIV-1) replication. While most spliced mRNAs follow canonical gene expression pathway in which recruitment nuclear cap-binding complex (CBC) and exon junction (EJC) largely stimulates rates export translation, relies on protein Rev to reach cytoplasm recruit host translational machinery. Here, we confirm that ensures high levels by driving...
Abstract Control of the COVID-19 pandemic largely depends on effectiveness vaccination. Several factors including vaccine hesitancy can affect vaccination process. Understanding that underlie willingness to accept brings pivotal information control pandemic. We analyzed association between level vaccine, and determinants amidst Chilean Individual-level survey data was collected from nationally representative samples 744 respondents, multivariate regression models used estimate outcome...
ABSTRACT During retroviral replication, the full-length RNA serves both as mRNA and genomic (gRNA). While simple retrovirus MLV segregates its into two functional populations, HIV-1 was proposed to exist a single population used indistinctly for protein synthesis or packaging. However, mechanisms by which Gag selects molecules that will be packaged nascent virions remain poorly understood. Here, we demonstrate packaging is regulated through an epitranscriptomic switch requiring demethylation...
ABSTRACT Translation initiation of the human immunodeficiency virus type-1 (HIV-1) unspliced mRNA has been shown to occur through cap-dependent and IRES-driven mechanisms. Previous studies suggested that nuclear cap-binding complex (CBC) rather than eIF4E drives translation we have recently reported CBC subunit CBP80 supports function viral protein Rev during export this transcript. Ribosome recruitment CBC-dependent cellular mRNAs relies on activity CBP80/20 factor (CTIF), which bridges 40S...