A5021175012- RNA Research and Splicing
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- Surfactants and Colloidal Systems
- RNA regulation and disease
- Computational Drug Discovery Methods
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Zoonotic diseases and public health
- Chemical and Physical Properties in Aqueous Solutions
- Protein Interaction Studies and Fluorescence Analysis
- Viral gastroenteritis research and epidemiology
University of Chile
2018-2025
Millennium Institute on Immunology and Immunotherapy
2025
Universidad Bernardo O'Higgins
2023
University of Talca
2015
During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, mechanisms by which HIV-1 Gag protein selects two molecules that will be packaged into nascent virions remain poorly understood. Here, we demonstrate deposition of N6-methyladenosine (m6A) regulates packaging. While m6A METTL3/METTL14 onto was associated with increased synthesis reduced packaging, FTO-mediated demethylation promoted incorporation viral particles. Interestingly, associates...
Gag synthesis from the full-length unspliced mRNA is critical for production of viral progeny during human immunodeficiency virus type-1 (HIV-1) replication. While most spliced mRNAs follow canonical gene expression pathway in which recruitment nuclear cap-binding complex (CBC) and exon junction (EJC) largely stimulates rates export translation, relies on protein Rev to reach cytoplasm recruit host translational machinery. Here, we confirm that ensures high levels by driving translation...
Antarctica is a unique environment due to its extreme meteorological and geological conditions. In addition this, relative isolation from human influences has kept it undisturbed. This renders our limited understanding of fauna associated microbial viral communities relevant knowledge gap fill. includes members the order Charadriiformes such as snowy sheathbills. They are opportunistic predator/scavenger birds distributed on Antarctic sub-Antarctic islands that in frequent contact with other...
Evidence from systems biology indicates that promiscuous drugs, i.e. those act simultaneously at various protein targets, are clinically better in terms of efficacy, than a more selective fashion. This has generated new trend drug development called polypharmacology. However, the rational design compounds is difficult task, particularly when drugs aimed to receptors with diverse structure, function and endogenous ligand. In present work, using docking molecular dynamics methodologies, we...
Translation initiation of the human immunodeficiency virus type-1 (HIV-1) full-length RNA has been shown to occur through cap-dependent and IRES-driven mechanisms. Previous studies suggested that nuclear cap-binding complex (CBC) rather than eIF4E drives translation we have recently reported CBC subunit CBP80 supports function viral protein Rev during export this transcript. Ribosome recruitment CBC-dependent cellular mRNAs relies on activity CBP80/20 factor (CTIF), which bridges 40S...
Abstract Gag synthesis from the full-length unspliced mRNA is critical for production of viral progeny during human immunodeficiency virus type-1 (HIV-1) replication. While most spliced mRNAs follow canonical gene expression pathway in which recruitment nuclear cap-binding complex (CBC) and exon junction (EJC) largely stimulates rates export translation, relies on protein Rev to reach cytoplasm recruit host translational machinery. Here, we confirm that ensures high levels by driving...
ABSTRACT During retroviral replication, the full-length RNA serves both as mRNA and genomic (gRNA). While simple retrovirus MLV segregates its into two functional populations, HIV-1 was proposed to exist a single population used indistinctly for protein synthesis or packaging. However, mechanisms by which Gag selects molecules that will be packaged nascent virions remain poorly understood. Here, we demonstrate packaging is regulated through an epitranscriptomic switch requiring demethylation...