A5042631847- Adenosine and Purinergic Signaling
- Cardiac Arrest and Resuscitation
- Respiratory Support and Mechanisms
- Mechanical Circulatory Support Devices
- Algal biology and biofuel production
- Drug Transport and Resistance Mechanisms
- Labor Law and Work Dynamics
- Neuroscience of respiration and sleep
- Aquaculture disease management and microbiota
- Effects of Radiation Exposure
- Digital Transformation in Industry
- Technology Use by Older Adults
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Human Rights and Immigration
- CRISPR and Genetic Engineering
- Family and Matrimonial Law
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Radiation Therapy and Dosimetry
- Arsenic contamination and mitigation
- Physiological and biochemical adaptations
- Environmental Policies and Emissions
- Nuclear Structure and Function
- Bacterial Infections and Vaccines
Nationwide Children's Hospital
2024
VTT Technical Research Centre of Finland
2023
Shanghai Jiao Tong University
2023
University of Oulu
2023
University of Iowa
2022-2023
Hospital Universitario Virgen del Rocío
2023
University of Kansas Medical Center
2021
Universidad de Santiago de Chile
2010-2020
University of Chile
2018-2019
Universidad Bernardo O'Higgins
2017-2018
There is wide variability in mechanical ventilation settings during extracorporeal membrane oxygenation (ECMO) patients with acute respiratory distress syndrome. Although lung rest recommended to prevent further injury, there no evidence support it.To determine whether near-apneic decreases injury a pig model of syndrome supported ECMO.Pigs (26-36 kg; n = 24) were anesthetized and connected ventilation. In 18 animals was induced by double-hit consisting repeated saline lavages followed 2...
Radiation-induced rectal epithelial damage is a very common side effect of pelvic radiotherapy and often compromise the life quality treatment outcome in patients with malignancies. Unlike small bowel colon, radiation stem cells has not been explored extensively. Here we demonstrate that Lgr5-positive are radiosensitive organoid-based transplantation mitigates rectum.C57Bl6 male mice (JAX) at 24 h were exposed to irradiation (PIR) determine epithelium. Effect PIR on (RSCs) was determined...
Gastric Cancer (GC) is the one of most prevalent and leading causes cancer-induced deaths. Previously, we have found that purinergic P2Y2 receptor (P2Y2R) expression increased in GC samples as compared to adjacent healthy mucosa taken from GC-diagnosed patients. In this work, studied detail signaling gastric adenocarcinoma-derived cell lines, AGS, MKN-45 MKN-74 non-tumoral epithelial line, GES-1. GC-derived cells, detected several receptors, important differences GES-1 cells. Functional...
Abstract Background The marine alga Ulva compressa is the dominant species in copper-polluted coastal areas northern Chile. It has been shown that tolerates micromolar concentrations of copper and accumulates at intracellular level. Transcriptomic analyses were performed using total RNA cultivated with 10 μ M for 0, 1, 3 5 days RNA-seq order to identify processes involved tolerance. Results levels transcripts encoding proteins belonging Light Harvesting Complex II (LHCII), photosystem...
In this study, we demonstrate the identification of an internal ribosome entry site (IRES) within 5'-untranslated region (5'-UTR) mouse mammary tumor virus (MMTV). The 5'-UTR full-length mRNA derived from infectious, complete MMTV genome was cloned into a dual luciferase reporter construct containing upstream Renilla gene (RLuc) and downstream firefly (FLuc). rabbit reticulocyte lysate, capable driving translation second cistron. vitro translational activity resistant to addition m(7)GpppG...
Arsenic has been associated with multiple harmful effects at the cellular level. Indirectly these defects could be related to impairment of integrity immune system, in particular lymphoid population. To characterize effect on redox status this population, copper smelter workers and arsenic unexposed donors were recruited for study. We analyzed urine samples lymphocyte enriched fractions from determinate levels proliferation. Moreover, we studied presence oxidative markers MDA, vitamin E SOD...
The marine alga Ulva compressa is the dominant species in coastal areas receiving effluents from copper mines. can accumulate high amounts of and possesses a strong antioxidant system. Here, we performed short-term transcriptomic analyses using total RNA cultivated with 10 μM for 0, 3, 6, 12 24 h by RNA-seq.De novo transcriptomes were assembled Trinity software, putative proteins annotated classified Blast2GO. Differentially expressed transcripts identified edgeR. Transcript levels compared...
As neuroactive steroids modulate several ionotropic receptors, we assessed whether the ATP-gated currents elicited by P2X(4) receptors are modulated these compounds. We transfected HEK293 cells or injected Xenopus laevis oocytes with cDNA coding for rat receptor. Application of 0.1-10 microM alfaxolone potentiated within 60-s 1 ATP-evoked a maximal potentiation 1.8 and 2.6-fold in respectively. Allopregnalolone 3alpha, 21-dihydroxy-5alpha-pregnan-20-one (THDOC) also but effect only averaging...
Protein allosteric modulation is a pillar of metabolic regulatory mechanisms; this concept has been extended to include ion channel regulation. P2XRs are ligand-gated channels activated by extracellular ATP, sensitive trace metals and other chemicals. By combining in silico calculations with electrophysiological recordings, we investigated the molecular basis P2X4R Zn(II) ivermectin, an antiparasite drug currently used veterinary medicine. To aim, docking studies, dynamics simulations...
Barrier-to-autointegration factor (BAF) is an essential component of the nuclear lamina. Encoded by BANF1, this DNA binding protein contributes to regulation gene expression, cell cycle progression, and integrity. A rare recessive BAF variant, Ala12Thr, causes premature aging syndrome, Néstor–Guillermo progeria syndrome (NGPS). Here, we report first dominant pathogenic Gly16Arg, identified in a patient presenting with progressive neuromuscular weakness. Although disease variants carry nearby...
<p>In this article, the emerging security challenges in communications of technologies Industry X are identified, discussed, and evaluated to motivate future research direction.</p>
The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. bacterial endotoxin lipopolysaccharide (LPS) is one of strongest stimuli it has been shown that P2X7R activation can modulate LPS-induced responses. Moreover, a C-terminal binding site for LPS proposed. In order to evaluate if directly activity P2X7R, we tested signaling pathways associated with HEK293 cells do not express...
Active thermography with inductive excitation is proposed as a reliable method for substitution of magnetic particle inspection on steel automotive components.A study inner part the component presented, where typical superficial cracks different length (>5 mm) and orientation are detected by induction thermography.Programmed acquisition analysis Fourier transformation developed to obtain phase image crack detection.Image processing can be detection within geometrical indications.Short...