A5088150009- Cancer, Hypoxia, and Metabolism
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Nanoplatforms for cancer theranostics
- Redox biology and oxidative stress
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Parvovirus B19 Infection Studies
- Bioactive Compounds and Antitumor Agents
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Sulfur Compounds in Biology
- Free Radicals and Antioxidants
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mitochondrial Function and Pathology
- Glutathione Transferases and Polymorphisms
- Animal Disease Management and Epidemiology
- ATP Synthase and ATPases Research
- Mass Spectrometry Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Genomics, phytochemicals, and oxidative stress
University of California, San Diego
2025
Illinois Department of Public Health
2022-2023
University of Illinois Urbana-Champaign
2012-2017
Center for Genomic Science
2012
As part of the response to highly pathogenic avian influenza A(H5N1) virus outbreak in U.S. cattle and poultry associated human cases, CDC partners are monitoring A levels detection H5 subtype wastewater. Among 48 states District Columbia that performed testing wastewater during May 12-July 13, 2024, a weekly average 309 sites 38 had sufficient data for analysis, 11 four reported high virus. was conducted at 203 41 states, with detections 24 nine states. For each or level, state local health...
Proteins with nuclear localization sequences (NLSs) are directed into the cell nucleus through interactions between NLS and importin proteins. NLSs generally short motifs rich in basic amino acids; however, identifying can be challenging due to lack of a universally conserved sequence. In this study, we characterized sequence specificity an essential Mcm3, subunit replicative DNA helicase. Through mutagenesis AlphaFold 3 (AF3) modeling, demonstrate that precise positioning residues within is...
The glutathione thiol/disulfide couple is the major redox buffer in endoplasmic reticulum (ER); however, mechanisms by which it contributes to tightly regulated environment of this intracellular organelle are poorly understood. recent development genetically encoded, ratiometric, single green fluorescent protein-based redox-sensitive (roGFP) sensors adjusted for more oxidative environments enables non-invasive measurement ER living cells. In turn, Förster resonance energy transfer (FRET)...
Regions of hypoxia are common in solid tumors and associated with enhanced malignancy, metastasis, chemo/radio resistance. Real-time hypoxic cellular experimentation is challenging due to the constant need for oxygen control. Most microfluidic platforms developed thus far cell studies burdened by complex design parameters difficult use uninitiated investigators. However, open-well enable short long term an ease workflow. Specifically, manipulation addition cells, media, reagents using a...
ABSTRACT As of August 2022, cases acute severe hepatitis unknown etiology in children have been reported from 35 countries, including the United States. Here we used PCR testing, viral enrichment based sequencing, and agnostic metagenomic sequencing to analyze 27 samples, nasopharyngeal swab, stool, plasma, and/or whole blood, 16 such 6 states (Alabama, California, Florida, Illinois, North Carolina, South Dakota) presenting October 1, 2021 May 22, parallel with blood samples 45 controls....
Quantitative phase imaging has been used in the past to study dry mass of cells and cell growth under various treatment conditions. However, relationship between cellular redox rates not yet studied this context. This employed recombinant Glrx-roGFP2 biosensor targeted mitochondrial matrix or cytosolic compartments A549 lung epithelial carcinoma cells. The Glrx-roGFP2s consists a modified GFP protein containing internal cysteine residues sensitive local environment. formation/dissolution...
Abstract Glioblastoma multiforme (GBM) is the most common and malignant form of brain cancer in adults, with a median survival time 12-15 months. Characterizing metabolic alterations GBM crucial step towards identifying potential targets for treatment, mitochondria, electron transport chain, associated reactive oxygen species presenting lucrative therapy. However, Warburg effect, which cells generate ATP mainly via glycolysis despite adequate functional can render resistant to antagonists...