A5086390052- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Livestock and Poultry Management
- Viral Infections and Vectors
- Microbial infections and disease research
- Fish Ecology and Management Studies
- Plant and Fungal Interactions Research
- Genomics and Phylogenetic Studies
- T-cell and Retrovirus Studies
- Hepatitis Viruses Studies and Epidemiology
- Infection Control and Ventilation
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Myxozoan Parasites in Aquatic Species
- Microbial Metabolism and Applications
South Dakota State University
2023-2025
University of Minnesota
2015-2024
University of Minnesota System
2020-2022
Minnesota Department of Natural Resources
2021
University of Chile
2017
Gaertner (United States)
2017
Avian metapneumovirus (aMPV), classified within the Pneumoviridae family, wreaks havoc on poultry health. It typically causes upper respiratory tract and reproductive infections, mainly in turkeys, chickens, ducks. Four subtypes of AMPV (A, B, C, D) two unclassified have been identified, which A B are widely distributed across world. In January 2024, an outbreak severe disease occurred turkey chicken farms different states US. Metagenomics sequencing selected tissue swab samples confirmed...
ABSTRACT Laboratory studies of virus aerosols have been criticized for generating airborne viruses from artificial nebulizer suspensions (e.g., cell culture media), which do not mimic the natural release human saliva). The objectives this study were to determine effect saliva on infectivity and survival compare it with those medium. A stock MS2 bacteriophage was diluted in one three suspensions, aerosolized, size selected (100 450 nm) using a differential mobility analyzer, collected onto...
Possible mechanisms that lead to inactivation of feline calicivirus (FCV) by cold atmospheric-pressure plasma (CAP) generated in 99% argon-1% O2 admixture were studied. We evaluated the impact CAP exposure on FCV viral capsid protein and RNA employing several cultural, molecular, proteomic morphologic characteristics techniques. In case long (2 min) CAP, reactive species strongly oxidized major domains (VP1) leading disintegration a majority capsids. short (15 s), some virus particles...
Astrovirus has been reported to be associated with diarrhea in pigs. The current study was conducted for the detection and molecular characterization of astroviruses diarrheic pigs submitted Veterinary Diagnostic Laboratory, University Minnesota. Intestinal contents from 269 were examined by reverse transcription polymerase chain reaction (RT-PCR), 62% found positive astroviruses. Of samples, 20% astrovirus alone while rotavirus detected 58% samples. remaining 22% revealed presence along...
Foot and mouth disease virus (FMDV) was isolated from sloughed tongue epithelium of Egyptian cattle presenting with lesions ropy salivation in two governorates (El-Fayoum Dakahlia). The Madin-Darby bovine kidney (MDBK) cells identified by reverse transcription-polymerase chain reaction (RT-PCR). complete genome obtained next generation sequencing. strains El-Fayoum Dakahlia were serotype A O, respectively both isolates had identity the previously reported strains. This study reports...
During the spring and summer of 2011, Minnesota Veterinary Diagnostic Laboratory at University received 14 submissions 15-to-18-week-old tom turkeys that were recumbent with wing tip bruises ("wing walkers") uni- or bilateral swelling hock (tibiotarsal) joints. Gastrocnemius digital flexor tendons occasionally ruptured. A total five turkey arthritis reoviruses (TARV-MN1 through TARV-MN5) isolated in specific-pathogen-free embryonated chicken eggs QT-35 cells. The identity isolates was...
Two studies were conducted to determine the role of enteric viruses in Light Turkey Syndrome (LTS), which is characterized by lower weight market age turkeys than their standard breed character. In surveillance study, we selected four LTS and two non-LTS turkey flocks Minnesota collected faecal samples at 2, 3, 5 8-weeks age. Astrovirus, rotavirus, reovirus detected alone or various combinations both flocks. No coronavirus was no corona- second 2-week-old poults divided into groups; Group A...
During both regulatory and routine surveillance sampling of baitfish from the states Illinois, Minnesota, Montana, Wisconsin, USA, isolates (n = 20) a previously unknown picornavirus were obtained kidney/spleen or entire viscera fathead minnows (Pimephales promelas) brassy (Hybognathus hankinsoni). Following appearance diffuse cytopathic effect, examination cell culture supernatant by negative contrast electron microscopy revealed presence small, round virus particles (∼30–32 nm), with...
Turkey arthritis reovirus (TARV) has been isolated from the gastrocnemius tendons and tibiotarsal joint fluid of lame male turkeys >12 weeks old in Midwest. Two experiments were conducted to compare pathogenicity three TARVs (TARV-MN2, TARV-MN4 TARV-O'Neil), one turkey enteric (TERV strain MN1) chicken (CARV MN1). hundred microlitres virus inoculated by oral, intratracheal, or footpad route into 6-day-old poults placed isolator units. Poults necropsied at 1 4 post infection Experiment 1, 2...
We identified 3 novel and distinct avulaviruses from Gentoo penguins sampled in Antarctica. isolated these viruses sequenced their complete genomes; serologic assays demonstrated that the do not have cross-reactivity between them. Our findings suggest new represent members of avulavirus species.
Koi herpesvirus (KHV; cyprinid herpesvirus-3) and carp oedema virus (CEV) are important viruses of common koi (Cyprinus carpio); however, the distribution these in wild North America is largely unknown. During summers 2017 2018, 27 mass mortalities were reported from four states USA (Minnesota, Iowa, Pennsylvania Wisconsin), majority which distributed across eight major watersheds southern Minnesota. Samples 22 mortality events five clinically healthy nearby populations screened for KHV, CEV...
Diarrhea caused by Escherichia coli in calves is an important problem terms of survivability, productivity and treatment costs. In this study, 88 150 diarrheic animals tested positive for E. coli. Of these, 54 samples had mixed infection with other bacterial and/or parasitic agents. There are several diarrheagenic pathotypes including enteropathogenic (EPEC), Shiga-toxin producing (STEC), enterotoxigenic (ETEC) necrotoxigenic (NTEC). Molecular detection virulence factors Stx2, Cdt3, Eae,...
Totiviridae is a virus family well known to infect uni-cellular organisms like fungi and protozoa. In more recent years, viruses characterized as toti-like viruses, have been found in primarily arthropods, but also couple planarians piscine species. These share phylogenetic similarities totiviruses; however, their genomes includes additional coding sequences either 5′ or 3′ ends expected relate advanced infection mechanisms hosts. Here, we applied next generation sequencing (NGS)...
Abstract Background Newcastle disease (ND) is an economically important viral affecting the poultry industry. In Kerala, a state in South India, incidences of ND commercial and backyard have been reported. But systematic statewide study on prevalence has not carried out. Objectives A cross‐sectional survey was performed to detect presence virus (NDV) suspect cases among apparently healthy flocks poultry, identify risk factors for NDV infection. Methods Real‐time reverse transcription‐PCR...
Newcastle disease (ND), caused by virus (NDV), is a contagious that affects variety of domestic and wild avian species. Though ND vaccine-preventable, it persistent threat to poultry industry across the globe. The represents leading cause morbidity mortality in chickens. To better understand epidemiology NDV among commercial backyard chickens Odisha, where chicken farming being prioritized assist with poverty alleviation, cross-sectional study was conducted two distinct seasons during 2018....
Abstract Spring viremia of carp virus (SVCV) causes a highly contagious and serious disease freshwater cyprinid fishes, generating significant economic ecological impacts throughout the world. The SVCV is therefore listed as notifiable pathogen by International Organization for Animal Health. In June 2011, mortality event wild common Cyprinus carpio occurred in Minnehaha Creek near its confluence with Mississippi River Pool 2 Minneapolis, Minnesota. Clinical signs moribund fish included...
Heterosporosis is an increasingly important microsporidian disease worldwide, impacting wild and farmed raised fishes in both marine freshwater environments. A previously undescribed species (Heterosporis sp.), with widespread distribution the Great Lakes region, was subject of this study. Three angler-caught fish were submitted to Minnesota Veterinary Diagnostic Laboratory from 2009-2010 lesions caused by intracellular proliferation parasitic spores, resulting destruction eventual necrosis...
The detection and co-circulation of multiple variants porcine reproductive respiratory syndrome virus (PRRSV) have been observed reported in swine. However, the potential long-term impact prevailing PRRSV on pig-performance is not yet fully understood. primary objective this study was to describe genetic variation processing fluid (PF), oral (OF), tonsil scraping (TS) specimens from five swine farms with different production types PRRS status over a period time (~1 year). Furthermore,...