A5013503265- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- Microbial Metabolites in Food Biotechnology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Meat and Animal Product Quality
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Antibiotics Pharmacokinetics and Efficacy
- Gut microbiota and health
- Parasite Biology and Host Interactions
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Mycobacterium research and diagnosis
- Synthesis of β-Lactam Compounds
- Animal Genetics and Reproduction
- Steroid Chemistry and Biochemistry
- Insect and Pesticide Research
- Metabolism and Genetic Disorders
Universidad Complutense de Madrid
1997-2024
Emory University
2024
Children's Healthcare of Atlanta
2024
Hospital Clínico Universitario de Valladolid
2009-2023
Kinshasa General Hospital
2023
Universidad de León
1996-2019
Universidad de León
2018
Texas State University
2017
Centro de Ingeniería Genética y Biotecnología
2016
Universidad de Valladolid
2008
This study assessed 16 different honey samples in order to select the best one for therapeutic purposes. First, a of honey's main bioactive compounds was carried out. Then phenolic profiles were determined and specific quantified using HPLC system coupled mass spectrometer. Then, antioxidant activity, by three vitro methods, antibacterial activity against reference strains clinical isolates evaluated. Great variability among observed regarding ascorbic acid (between 0.34 ± 0.00 75.8 0.41...
The gene product of open reading frame 5 (p25) porcine reproductive and respiratory syndrome (PRRS) virus has been expressed by coinfection culture cells with vaccinia expressing the T7 RNA polymerase a recombinant encoding under promoter encephalomyocarditis internal ribosome entry site. In spite reported efficiency expression system, very poor accumulation p25 protein was observed strong cytotoxicity produced in doubly infected cells. This cell toxicity shown to occur induction apoptosis,...
OPINION article Front. Microbiol., 12 March 2019Sec. Food Microbiology Volume 10 - 2019 | https://doi.org/10.3389/fmicb.2019.00375
Summary: Glucose exerted a concentration-dependent negative regulation on the biosynthesis of cephamycin C by Streptomyces lactamdurans. Formation precursor δ(α-aminoadipyl)-cysteinyl-valine was greatly decreased excess glucose. The ring-expanding enzyme deacetoxycephalosporin synthase strongly repressed glucose in vivo. Isopenicillin N (cyclase) and isopenicillin epimerase were not However, activity inhibited vitro 6-phosphate, inorganic phosphate, fructose 2,6-diphosphate 1,6-diphosphate....
SUMMARY: The deacetoxycephalosporin C (DAOC) synthase (expandase) of Streptomyces lactamduranswas highly purified, as shown by SDS-PAGE and isoelectric focusing. enzyme catalysed the oxidative ring expansion that converts penicillin N into DAOC. was very unstable but could be partially stabilized in 25 mm-Tris/HCl, pH 9·0, presence DTT (0·1 mm). required 2-oxoglutarate, oxygen Fe2 +, did not need ATP, ascorbic acid, Mg2 + or K+. optimum temperature between 30 °C. DAOC showed a high...
Traditional cheeses are an important reservoir of microbial diversity that can have biotechnological applications, especially with a view to improving the characteristics unique each type cheese, and in this respect, starter cultures consisting autochthonous lactic acid bacteria strains particular interest. In present study, we investigated population San Simón da Costa cheese (PDO, Galicia, Spain) found predominance genus Lactobacillus, which by end ripening accounted for 78% isolated...
This study consisted of a palynological, microbiological, and physicochemical characterization fifteen samples Spanish honey sold under quality brands with different botanical geographical origins from two consecutive harvest years (2010 2011). Eight the were classified as monofloral Persea americana, Castanea sativa, Rosmarinus officinalis, Eucalyptus sp., Thymus sp. With regard to microbiological analyses, mold, yeast counting, Staphylococcus aureus, Salmonella, sulfite-reducing...
A large number of species corynebacteria are known to be amino acid producers, including members the genera Corynebacterium and Brevibacterium. Pulsed-field gel electrophoresis (PFGE) DNA fragments obtained by using endonucleases which recognize AT-rich hexanucleotide or octanucleotide sequences produces a discrete pattern bands useful for fingerprinting physical mapping chromosome. Using Pacl Swal genome Brevibacterium lactofermentum ATCC 13869 (genome size 3052 kb) was consistently cut...
The isopenicillin N synthase (cyclase) of Streptomyces lactamdurans (syn. Nocardia lactamdurans) has been purified to near homogeneity as judged by SDS-PAGE and isoelectric focusing. This enzyme catalyses the oxidative cyclization tripeptide δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine N. required DTT, Fe2+ oxygen it was greatly stimulated ascorbic acid. It strongly inhibited Co2+, Zn2+ Mn2+. Optimal pH temperature were 7·0 25 °C (with assay conditions used), respectively. apparent K m for 0·18...
Isopenicillin N (IPN) epimerase, an enzyme involved in cephalosporin and cephamycin biosynthesis that converts IPN into penicillin N, was extracted from Nocardia lactamdurans purified 88-fold. The unstable but could be partially stabilized by addition of pyridoxal phosphate. did not require ATP for activity contrast to other amino acid racemases. had M r 59000 as determined gel filtration; epimerase Streptomyces clavuligerus 63000. A protein band found enriched SDS-PAGE active fractions N....