A5003796185- RNA Research and Splicing
- Nuclear Structure and Function
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Protein Structure and Dynamics
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Neurological disorders and treatments
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Biotin and Related Studies
- Parkinson's Disease Mechanisms and Treatments
- Microtubule and mitosis dynamics
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Viral Infectious Diseases and Gene Expression in Insects
- Metabolism and Genetic Disorders
- Protein Degradation and Inhibitors
- Metalloenzymes and iron-sulfur proteins
European Molecular Biology Laboratory
2013-2023
ETH Zurich
2021-2023
New York University
2023
European Molecular Biology Laboratory
2017-2021
Highlights•The human NPC is resolved up to 3.2 nm•32 copies of the hNup107 subcomplex form two reticulated rings•Mitotic phosphorylation sites cluster into intersubcomplex contacts•The hNup214 forms a protrusion at cytoplasmic ringSummaryThe nuclear pore complex (NPC) fundamental component all eukaryotic cells that facilitates nucleocytoplasmic exchange macromolecules. It assembled from multiple about 30 nucleoporins. Due its size and composition, determining structure an enormous challenge,...
INTRODUCTION The eukaryotic nucleus pro-tects the genome and is enclosed by two membranes of nuclear envelope. Nuclear pore complexes (NPCs) perforate envelope to facilitate nucleocytoplasmic transport. With a molecular weight ∼120 MDa, human NPC one larg-est protein complexes. Its ~1000 proteins are taken in multiple copies from set about 30 distinct nucleoporins (NUPs). They can be roughly categorized into classes. Scaf-fold NUPs contain folded domains form cylindrical scaffold...
Article25 July 2017Open Access Transparent process Capturing protein communities by structural proteomics in a thermophilic eukaryote Panagiotis L Kastritis orcid.org/0000-0002-1463-8422 European Molecular Biology Laboratory, Structural and Computational Unit, Heidelberg, Germany Search for more papers this author Francis J O'Reilly Chair of Bioanalytics, Institute Biotechnology, Technische Universität Berlin, Thomas Bock orcid.org/0000-0002-9314-5318 Yuanyue Li orcid.org/0000-0001-5971-0355...
Nuclear pore complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes early embryos, NPCs reside not only within NE, but also at some endoplasmic reticulum (ER) membrane sheets, termed annulate lamellae (AL). Although a role for AL as NPC storage pools has been discussed, it remains controversial whether how they contribute to density NE. Here, we show that insert into NE ER feeds rapid expansion in Drosophila blastoderm embryos. We...
Article18 December 2017Open Access Transparent process Landscape of nuclear transport receptor cargo specificity Marie-Therese Mackmull orcid.org/0000-0003-2928-1144 Structural and Computational Biology Unit, European Molecular Laboratory, Heidelberg, Germany Search for more papers by this author Bernd Klaus Centre Statistical Data Analysis, Ivonne Heinze Leibniz Institute on Aging, Fritz Lipmann (FLI), Jena, Manopriya Chokkalingam Cellular Networks Systems Biology, CECAD, University...
Abstract Cancer-relevant signalling pathways rely on bidirectional nucleocytoplasmic transport events through the nuclear pore complex (NPC). However, mechanisms by which individual NPC components (Nups) participate in regulation of these remain poorly understood. We discover integrating large scale proteomics, polysome fractionation and a focused RNA i approach that Nup155 controls mRNA translation p21 ( CDKN1A ), key mediator p53 response. The underlying mechanism involves transcriptional...
Abstract Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport. Their intricate 120 MDa architecture remains incompletely understood. Here, we report a near-complete structural model of the human NPC scaffold with explicit membrane and in multiple conformational states. We combined AI-based structure prediction situ cellulo cryo-electron tomography integrative modeling. show that linker Nups spatially organize within across subcomplexes to establish higher-order structure....
Histone deacetylases (HDACs) and acetyltransferases control the epigenetic regulation of gene expression through modification histone marks. deacetylase inhibitors (HDACi) are small molecules that interfere with tail modification, thus altering chromatin structure epigenetically controlled pathways. They promote apoptosis in proliferating cells promising anticancer drugs. While some HDACi have already been approved for therapy others different phases clinical trials, exact mechanism action...
Gene duplication enables the emergence of new functions by lowering evolutionary pressure that is posed on ancestral genes. Previous studies have highlighted role specific paralog genes during cell differentiation, for example, in chromatin remodeling complexes. It remains unexplored whether similar mechanisms extend to other biological and regulation conserved across species. Here, we analyze expression paralogs human tissues, development neuronal differentiation fish, rodents humans....
Journal Article Integrative Structural Analysis of Human Nuclear Pore Complex Get access Shyamal Mosalaganti, Mosalaganti Department Molecular Sociology, Max Planck Institute Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, GermanyStructural and Computational Biology Unit, European Laboratory, Meyerhofstraße 1, 69117, Heidelberg, GermanyLife Sciences Institute, Cell & Developmental Biology, University Michigan, Ann Arbor, MI 48109, USA Search for other works by this author on:...
Abstract Gene duplication enables the emergence of new functions by lowering general evolutionary pressure. Previous studies have highlighted role specific paralog genes during cell differentiation, e.g., in chromatin remodeling complexes. It remains unexplored whether similar mechanisms extend to other biological and regulation is conserved across species. Here, we analyze expression paralogs human tissues, development neuronal differentiation fish, rodents humans. While ~80% are...