A5015906254- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Nuclear Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Cellular transport and secretion
- DNA Repair Mechanisms
- Platelet Disorders and Treatments
- Microbial Natural Products and Biosynthesis
- Long-Term Effects of COVID-19
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Epigenetics and DNA Methylation
- Nanoparticles: synthesis and applications
- Entomopathogenic Microorganisms in Pest Control
- HIV Research and Treatment
- Infectious Encephalopathies and Encephalitis
- Coral and Marine Ecosystems Studies
- Plant and fungal interactions
- Advanced X-ray Imaging Techniques
- Insects and Parasite Interactions
European Molecular Biology Laboratory
2010-2024
European Molecular Biology Laboratory
2009-2023
European Molecular Biology Organization
2012-2015
All positive strand RNA viruses are known to replicate their genomes in close association with intracellular membranes. In case of the hepatitis C virus (HCV), a member family Flaviviridae, infected cells contain accumulations vesicles forming membranous web (MW) that is thought be site viral replication. However, little about biogenesis and three-dimensional structure MW. this study we used combination immunofluorescence- electron microscopy (EM)-based methods analyze structures induced by...
The development of the endomembrane system was a major step in eukaryotic evolution. Membrane coats, which exhibit unique arrangement β-propeller and α-helical repeat domains, play key roles shaping membranes. Such proteins are likely to have been present ancestral eukaryote but cannot be detected prokaryotes using sequence-only searches. We used structure-based detection protocol search all proteomes for with this domain architecture. Apart from eukaryotes, we identified protein...
The apparently complex membrane organization of Gemmata obscuriglobus, and probably all PVC superphylum members, comprises interconnected invaginations is topologically identical to the "classical" Gram-negative bacterial system.
Nuclear pore complexes (NPCs) span the nuclear envelope (NE) and mediate nucleocytoplasmic transport. In metazoan oocytes early embryos, NPCs reside not only within NE, but also at some endoplasmic reticulum (ER) membrane sheets, termed annulate lamellae (AL). Although a role for AL as NPC storage pools has been discussed, it remains controversial whether how they contribute to density NE. Here, we show that insert into NE ER feeds rapid expansion in Drosophila blastoderm embryos. We...
The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore and is localized to kinetochores mitosis. Here we report that Seh1, component complex, functions chromosome alignment segregation by regulating centromeric localization Aurora B other passenger proteins. Localization CENP-E not affected Seh1 depletion analysis electron microscopy showed microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired localization, which results...
Abstract Background Extracellular vesicles (EVs) have been implicated in the pathogenesis of asthma, however, how EVs contribute to immune dysfunction and type 2 airway inflammation remains incompletely understood. We aimed elucidate roles their miRNA cargo NSAID‐exacerbated respiratory disease (N‐ERD), a severe inflammatory condition. Methods were isolated from induced sputum or supernatants cultured nasal polyp turbinate tissues N‐ERD patients healthy controls by size‐exclusion...
We report the presence of a membranous tubulovesicular network in planctomycete bacterium Gemmata obscuriglobus. This endomembrane system interacts with membrane coat proteins and is capable protein internalization degradation. Taken together, this suggests that planctomycetal may illuminate emergence complex systems.
MINT‐7951306, MINT‐7951362: pom121 (uniprotkb:Q5EWX9) physically interacts (MI:0914) with nup155 (uniprotkb:Q7ZWL0), nup160 (uniprotkb:P83722), nup93 (uniprotkb:Q7ZX96), Importin beta (uniprotkb:P52297) and nup62 (uniprotkb:Q91349) by pull down (MI:0096)
Production of RanGTP around chromosomes induces spindle assembly by activating nuclear localization signal (NLS)–containing factors. Here, we show that the NLS protein ISWI, a known chromatin-remodeling ATPase, is RanGTP-dependent microtubule (MT)-associated protein. Recombinant ISWI MT nucleation, stabilization, and bundling in vitro. In Xenopus culture cells egg extract, localizes within nucleus interphase on spindles during mitosis. Depletion extracts does not affect assembly, but...
Changes in cell morphology require coordination of plasma membrane turnover and cytoskeleton dynamics, processes that are regulated by Rho GTPases. Here, we describe how a direct interaction between the GTPase Cdc42 activating protein (GAP) GRAF1, facilitate rapid surface at leading edge. Both GRAF1 were required for fluid phase uptake generation transient GRAF1-coated endocytic carriers, distinct from clathrin coated vesicles. was found to transiently assemble discrete Cdc42-enriched...
Rationale: Intrinsic brain tumors, such as gliomas are largely resistant to immunotherapies including immune checkpoint blockade. Adoptive cell therapies (ACT) chimeric antigen receptor (CAR) or T (TCR)-transgenic therapy targeting glioma-associated antigens an emerging field in glioma immunotherapy. However, imaging techniques for non-invasive monitoring of adoptively transferred cells homing the microenvironment currently lacking. Methods: Ultrasmall iron oxide nanoparticles (NP) can be...
ABSTRACT The mechanism of endoplasmic reticulum (ER) morphogenesis is incompletely understood. ER tubules are shaped by the reticulons (RTNs) and DP1/Yop1p family members, but sheet formation much less clear. Here, we characterize TMEM170A, a human transmembrane protein, which localizes in nuclear envelope membranes. Silencing or overexpressing TMEM170A HeLa K cells alters shape morphology. Ultrastructural analysis reveals that downregulation specifically induces tubular formation, whereas...
Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here, we describe chains of interconnecting vesicles protruding from cells strain Hel3_A1_48, affiliating Formosa spp. within the Flavobacteriia and originating coastal free-living bacterioplankton. were up to 10 μm long had emanating outer membrane a single size 80 100 nm by 50 nm. Cells extruded tubes exponential...
Epibiotic bacteria are known to live on and off bacterial cells. Here, we describe the ultramicrobacterial anaerobic epibiont OP3 LiM living Archaea Bacteria .
Abstract Microtubules are key to multiple neuronal functions involving the transport of organelles, however, their relationship neurotransmitter release is still unresolved. Here, we show that microtubules present in presynaptic compartment cholinergic autaptic synapses dynamic. To investigate how balance between microtubule growth and shrinkage affects neurotransmission induced synchronous depolymerization by photoactivation chemical inhibitor SBTub3. The consequence was an increase...
Due to its high resolution, electron microscopy (EM) is an indispensable tool for virologists. However, one of the main difficulties when analyzing virus-infected or transfected cells via EM are low efficiencies infection transfection, hindering examination these cells. In order overcome this difficulty, light (LM) can be performed first allocate subpopulation infected Thus, taking advantage use fluorescent proteins (FPs) fused viral proteins, LM used here record positions...