A5048854682- Yersinia bacterium, plague, ectoparasites research
- Vibrio bacteria research studies
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Bacillus and Francisella bacterial research
- Glycosylation and Glycoproteins Research
- Bacterial biofilms and quorum sensing
- Pharmacological Effects of Natural Compounds
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
- Bacterial Genetics and Biotechnology
- Listeria monocytogenes in Food Safety
- Caveolin-1 and cellular processes
- Retinal Development and Disorders
Umeå University
2015-2023
Bacterial membrane vesicle (MV) production has been mainly studied in Gram-negative species. In this study, we show that Listeria monocytogenes, a Gram-positive pathogen causes the food-borne illness listeriosis, produces MVs both vitro and vivo. We found major virulence factor, pore-forming hemolysin listeriolysin O (LLO), is tightly associated with MVs, where it resides an oxidized, inactive state. Previous studies have shown LLO may induce cell death autophagy. To monitor possible effects...
Changes in cell morphology require coordination of plasma membrane turnover and cytoskeleton dynamics, processes that are regulated by Rho GTPases. Here, we describe how a direct interaction between the GTPase Cdc42 activating protein (GAP) GRAF1, facilitate rapid surface at leading edge. Both GRAF1 were required for fluid phase uptake generation transient GRAF1-coated endocytic carriers, distinct from clathrin coated vesicles. was found to transiently assemble discrete Cdc42-enriched...
Adaptation of cell shape and polarization through the formation retraction cellular protrusions requires balancing endocytosis exocytosis combined with fine-tuning local activity small GTPases like Rab8. Here, we show that endocytic turnover plasma membrane at is directly coupled to surface removal inactivation Removal induced by reduced tension mediated GTPase regulator associated focal adhesion kinase-1 (GRAF1, also known as ARHGAP26), a clathrin-independent endocytosis. GRAF1-depleted...
Type III secretion systems harbored by several Gram-negative bacteria are often used to deliver host-modulating effectors into infected eukaryotic cells. About 20 core proteins needed for assembly of a apparatus. Several these genetically and functionally conserved in type associated with invertebrate or vertebrate hosts. In the Ysc family two poorly characterized protein families, YscX YscY family. plasmid-encoded Ysc-Yop system human pathogenic Yersinia species, is secreted substrate while...
The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. CpxA sensor kinase and CpxR response regulator two-component system is one such pathway. Active Cpx signalling upregulates various factors designed to repair restore integrity. Concomitantly, this pathway also down-regulates key determinants virulence. In Yersinia, cpxA deletion accumulates high levels...
Translocon pores formed in the eukaryotic cell membrane by a type III secretion system facilitate translocation of immune-modulatory effector proteins into host interior. The YopB and YopD produced secreted pathogenic Yersinia spp. harboring virulence plasmid-encoded perform this pore-forming translocator function. We had previously characterized vitro T3SS function vivo pathogenicity number strains encoding sited-directed point mutations yopD. This resulted classification mutants three...