A5059300499- Helicobacter pylori-related gastroenterology studies
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Eosinophilic Esophagitis
- DNA Repair Mechanisms
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Clostridium difficile and Clostridium perfringens research
- Molecular Biology Techniques and Applications
- Mitochondrial Function and Pathology
- Toxic Organic Pollutants Impact
- DNA and Nucleic Acid Chemistry
- Gastroesophageal reflux and treatments
- Carcinogens and Genotoxicity Assessment
- Geochemistry and Elemental Analysis
- Muscle Physiology and Disorders
- Bacillus and Francisella bacterial research
- Effects and risks of endocrine disrupting chemicals
- Urban Planning and Landscape Design
- DNA and Biological Computing
- Biotin and Related Studies
- Advanced Proteomics Techniques and Applications
- Environmental Toxicology and Ecotoxicology
- Viral Infections and Outbreaks Research
Umeå University
2011-2024
Umeå Plant Science Centre
2022
Olink Bioscience (Sweden)
2021
Sandvik (Sweden)
2013-2016
Uppsala University
2005-2016
Örebro University
2013-2016
Understanding the dynamics of human proteome is crucial for developing biomarkers to be used as measurable indicators disease severity and progression, patient stratification, drug development. The Proximity Extension Assay (PEA) a technology that translates protein information into actionable knowledge by linking protein-specific antibodies DNA-encoded tags. In this report we demonstrate how have combined unique PEA with an innovative automated sample preparation high-throughput sequencing...
Contamination of toxic spore-forming bacteria is problematic since spores can survive a plethora disinfection chemicals and it hard to rapidly detect if the chemical has inactivated spores. Thus, robust decontamination strategies reliable detection methods identify dead from viable are critical. In this work, we investigate changes Bacillus thuringiensis treated with sporicidal agents such as chlorine dioxide, peracetic acid, sodium hypochlorite using laser tweezers Raman spectroscopy. We...
Infectious diseases are often initiated by microbial adherence that is mediated the binding of attachment molecules, termed adhesins, to cell surface receptors on host cells. We present an experimental system, oblique-incidence reflectivity difference (OI-RD) microscopy, which allows detection novel, low-affinity mechanisms may be essential for infectious processes. OI-RD microscopy was used analyze direct oncopathogen, Helicobacter pylori ( H. ) immobilized glycoconjugates in real time with...
Changes in cell morphology require coordination of plasma membrane turnover and cytoskeleton dynamics, processes that are regulated by Rho GTPases. Here, we describe how a direct interaction between the GTPase Cdc42 activating protein (GAP) GRAF1, facilitate rapid surface at leading edge. Both GRAF1 were required for fluid phase uptake generation transient GRAF1-coated endocytic carriers, distinct from clathrin coated vesicles. was found to transiently assemble discrete Cdc42-enriched...
Abstract Background In this study we utilized padlock probes and rolling circle amplification as a mean to detect the replication of porcine circovirus type 2 (PCV2) in cultured cells infected tissue. Porcine is single-stranded circular DNA virus associated with several severe diseases, diseases (PCVD) pigs, such postweaning multisystemic wasting syndrome. The exact reason mechanisms behind trigger PCV2 that these not well-known. replicates thus also exists double-stranded replicative form....
Telomeres, the ends of linear chromosomes, are composed repetitive DNA sequences, histones and a protein complex called shelterin. How is packaged at telomeres an outstanding question in field with significant implications for human health disease. Here, we studied architecture their spatial association other chromatin domains different cell types using correlative light electron microscopy. To this end, shelterin TRF1 or TRF2 was fused tandem to eGFP peroxidase APEX2, which provided...
Cells rapidly lose their physiological phenotype upon disruption of extracellular matrix (ECM)-intracellular cytoskeleton interactions. By comparing adult mouse skeletal muscle fibers, isolated either by mechanical dissection or collagenase-induced ECM digestion, we investigated acute effects on cellular and mitochondrial morphology, transcriptomic signatures, Ca2+ handling. RNA-sequencing showed striking differences in gene expression patterns between the two isolation methods with...
Single cell gel electrophoresis, or the comet assay, is widely used to measure DNA damage and repair. However, behaviour of under conditions for assay not fully understood. In developing a method studying specific gene sequences within comets, using 'padlock probes' (circularizable oligonucleotide probes), we have first applied probes that hybridize Alu repetitive elements mitochondrial (mtDNA). During sequence stages in mtDNA progressively disperses into surrounding agarose gel, showing no...
Manganese oxides occur in a wide range of environmental settings either as coatings on rocks, sediment, and soil particles, or discrete grains. Although the production biologically mediated Mn is well established, relatively little known about microbial-specific strategies for utilizing environment how these affect morphology, structure, chemistry associated mineralizations. Defining such characterizing mineral properties would contribute to better understanding their impact local possibly...
The bacterial pathogen Chlamydia trachomatis employs the effector CpoS to suppress a host defense response that aborts intracellular growth by inducing cell death. While conducting CRISPR knock-out screen for genes contributing this response, we uncovered mutant deficient display markedly increased reliance on cellular ceramide synthesis, compensating its diminished ability acquire sphingolipids via modulating membrane trafficking. Employing power of just recently established molecular...
Many modern molecular labelling techniques result in bright point signals. Signals from molecules that are detected directly inside a cell can be captured by fluorescence microscopy. representing different types of may randomly distributed the cells or show systematic patterns, indicating corresponding have specific, non-random localisations and functions cell. Assessing this information requires high speed robust image segmentation followed signal detection, finally, pattern analysis. We...