A5053343387- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Dermatology and Skin Diseases
- Pediatric health and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Inhalation and Respiratory Drug Delivery
- Respiratory and Cough-Related Research
- FOXO transcription factor regulation
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tracheal and airway disorders
- Immune responses and vaccinations
- Transplantation: Methods and Outcomes
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Optimism, Hope, and Well-being
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Mast cells and histamine
- Helicobacter pylori-related gastroenterology studies
- Immune Cell Function and Interaction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
German Center for Lung Research
2016-2025
Helmholtz Zentrum München
2015-2024
Technical University of Munich
2015-2024
Center for Environmental Health
2024
Helmholtz Association of German Research Centres
2021-2022
ENT and Allergy
2020
Goethe University Frankfurt
2010-2016
Zentrum für Kinderheilkunde
2013
Jena University Hospital
2011
Boston Children's Hospital
2010
Interferon-γ (IFN-γ) and interleukin-4 (IL-4) are key effector cytokines for the differentiation of T helper type 1 2 (Th1 Th2) cells. Both induce fate-decisive transcription factors such as GATA3 TBX21 that antagonize polarized development opposite phenotypes by direct regulation each other's expression along with many other target genes. Although it is well established mesenchymal cells directly respond to Th1 Th2 cytokines, nature antagonistic programs in airway epithelial only partially...
Rationale In adults, personalised asthma treatment targets patients with type 2 (T2)-high and eosinophilic phenotypes. It is unclear whether such classification achievable in children. Objectives To define T2-high easily accessible biomarkers compare resulting phenotypes across all ages. Methods the multicentre clinical All Age Asthma Cohort (ALLIANCE), 1125 participants (n=776 asthmatics, n=349 controls) were recruited followed for years (1 year adults). Extensive characterisation...
BackgroundAllergen-specific immunotherapy (AIT) is a causative treatment in allergic airway disease, comprising long-term allergen administration and requiring three years of treatment. Mechanisms biomarkers that translate into clinical efficacy remain urgently needed.MethodsIn an exploratory observational allergy cohort we phenotyped 32 grass-pollen patients with hayfever undergoing AIT for over controls using local systemic samples ex vivo FACS, nasal transcriptomes vitro...
While several systemic immunomodulatory effects of allergen-specific immunotherapy (AIT) have been discovered, local anti-inflammatory mechanisms in the respiratory tract are largely unknown. We sought to elucidate and epithelial underlying a genome-wide approach.We induced sputum hay fever patients healthy controls during pollen peak season stratified by effective allergen or as untreated. Sputum was directly processed after induction subjected whole transcriptome RNA microarray analysis....
Extensive remodeling of the airways is a major characteristic chronic inflammatory lung diseases such as asthma or obstructive pulmonary disease (COPD). To elucidate importance deregulated immune response in for processes, we established matching Drosophila model. Here, triggering Imd (immune deficiency) pathway tracheal cells induced organ-wide remodeling. This structural comprises disorganization epithelial structures and comprehensive thickening. We show that these changes do not depend...
Children with asthma have impaired production of interleukin (IL) 37; in mice, IL-37 reduces hallmarks experimental allergic (EAA). However, it remains unclear how exerts its inhibitory properties asthma. This study aimed to identify the mechanism(s) by which controls inflammation.IL-37 target cells were identified single-cell RNA-seq IL-1R5 and IL-1R8. Airway tissues isolated laser-capture microdissection examined microarray-based gene expression analysis. Mononuclear (MNC) airway...
Abstract Background Extracellular vesicles (EVs) have been implicated in the pathogenesis of asthma, however, how EVs contribute to immune dysfunction and type 2 airway inflammation remains incompletely understood. We aimed elucidate roles their miRNA cargo NSAID‐exacerbated respiratory disease (N‐ERD), a severe inflammatory condition. Methods were isolated from induced sputum or supernatants cultured nasal polyp turbinate tissues N‐ERD patients healthy controls by size‐exclusion...
Studies show that proallergic TH 2 cells decrease after successful allergen-specific immunotherapy (AIT). It is likely iatrogenic administration of allergens drives these to exhaustion due chronic T-cell receptor stimulation. This study aimed investigate the T in connection with allergen exposure during AIT mice and two independent patient cohorts.OVA-sensitized C57BL/6J were challenged treated OVA, development local systemic was analyzed. In patients, expression exhaustion-associated...
TGF-β1 is known to have a pro-inflammatory impact by inducing Th9 and Th17 cells, while it also induces anti-inflammatory Treg cells (Tregs). In the context of allergic airway inflammation (AAI) its dual role can be critical importance in influencing outcome disease. Here we demonstrate that TGF-β major player AAI driving effector T Tregs differentiate independently. Induction experimental hyperreactivity mouse model with inducible genetic ablation gene encoding for TGFβ-receptor 2 ( Tgfbr2...
Background Comprehensive studies investigated the role of T-cells in asthma which led to personalised treatment options targeting severe eosinophilic asthma. However, little is known about contribution B-cells this chronic inflammatory disease. In study we various B-cell populations specific clinical features Methods All Age Asthma Cohort (ALLIANCE), a subgroup 154 adult patients and 28 healthy controls were included for characterisation by flow cytometry. Questionnaires, lung function...
Abstract Background Several microRNAs (miRs) have been described as potential biomarkers in liquid biopsies and the context of allergic asthma, while therapeutic effects on airway expression miRs remain elusive. In this study, we investigated epigenetic miR‐associated mechanisms sputum grass pollen‐allergic patients with without allergen‐specific immunotherapy (AIT). Methods Induced samples healthy controls (HC), AIT‐treated ‐untreated rhinitis (AA) asthma (AR) were profiled using miR...
Rationale: The strongest genetic risk factor for childhood-onset asthma, the 17q21 locus, is associated with increased viral susceptibility and disease-promoting processes.Objectives: To identify biological targets underlying escalated clinical phenotype mediated by locus.Methods: Genome-wide transcriptome analysis of nasal brush samples from 261 children (78 healthy, 79 wheezing at preschool age, 104 asthmatic) within ALLIANCE (All-Age-Asthma) cohort, a median age 10.0 (range, 1.0–20.0)...