A5054694861- Metabolomics and Mass Spectrometry Studies
- Hormonal Regulation and Hypertension
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer, Lipids, and Metabolism
- Molecular Biology Techniques and Applications
- Reproductive biology and impacts on aquatic species
- Endometrial and Cervical Cancer Treatments
- Hormonal and reproductive studies
- Physiological and biochemical adaptations
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Fish Ecology and Management Studies
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- Aldose Reductase and Taurine
- Pharmacogenetics and Drug Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Agricultural safety and regulations
- Adipokines, Inflammation, and Metabolic Diseases
- Reproductive System and Pregnancy
- Education, Achievement, and Giftedness
- Marine Sponges and Natural Products
- Psoriasis: Treatment and Pathogenesis
Helmholtz Zentrum München
2011-2025
Deutsches Diabetes-Zentrum e.V.
2020-2023
German Center for Diabetes Research
2020-2023
Heinrich Heine University Düsseldorf
2020-2023
University Hospital Heidelberg
2022
Heidelberg University
2022
Androgens are pleiotropic and play pivotal roles in the formation variation of sexual phenotypes. We show that differences circulating androgens between three male mating morphs ruff sandpipers linked to 17-beta hydroxysteroid dehydrogenase 2 (HSD17B2), encoded by a gene within supergene determines morphs. Low-testosterone males had higher HSD17B2 expression blood than high-testosterone males, as well brain areas related social behaviors testosterone production. Derived isozymes, which...
Global metabolomics analyses using body fluids provide valuable results for the understanding and prediction of diseases. However, mechanism a disease is often tissue-based it advantageous to analyze metabolomic changes directly in tissue. Metabolomics from tissue samples faces many challenges like collection, homogenization, metabolite extraction.We aimed establish extraction protocol optimized quantification by targeted AbsoluteIDQ™ p180 Kit (Biocrates). The method should be non-selective,...
Although cultured cells are nowadays regularly analyzed by metabolomics technologies, some issues in study setup and data processing still not resolved to complete satisfaction: a suitable harvesting method for adherent cells, fast robust normalization, the proof that metabolite levels can be normalized cell number.We intended develop normalization of culture samples, analyze how correlate with numbers, elucidate impact kind on measured profiles.We four different human lines used them...
Abstract Aberrant energy metabolism and cell cycle regulation both critically contribute to malignant growth processes represent targets for anticancer therapy. It is shown here that depletion of the AAA+‐ATPase thyroid hormone receptor interacting protein 13 (Trip13) results in mitotic death through a combined mechanism linking lipid aberrant mitosis. Diminished Trip13 levels hepatocellular carcinoma cells result insulin‐receptor‐/Akt‐pathway‐dependent accumulation droplets, which act as...
Stress, the physiological reaction to a stressor, is initiated in teleost fish by hormone cascades along hypothalamus-pituitary-interrenal (HPI) axis. Cortisol major stress and contributes appropriate response regulating gene expression after binding glucocorticoid receptor. inactivated when 11β-hydroxysteroid dehydrogenase (HSD) type 2 catalyzes its oxidation cortisone. In zebrafish, Danio rerio, cortisone can be further reduced 20β-hydroxycortisone. This catalyzed 20β-HSD 2, recently...
Citrate is important for lipid synthesis and epigenetic regulation in addition to ATP production. We have previously reported that cancer cells import extracellular citrate via the pmCiC transporter support their metabolism. Here, we show first time supplied by cancer-associated stroma (CAS) also release one of latter’s major metabolic tasks. from CAS controlled through cross-cellular communication. The availability regulated cytokine profile, metabolism features cellular invasion. Moreover,...
Zebrafish are widely used as model organism. Their suitability for endocrine studies, drug screening and toxicity assessements depends on the extent of conservation specific genes biochemical pathways between zebrafish human. Glucocorticoids consist inactive 11-keto (cortisone 11-dehydrocorticosterone) active 11β-hydroxyl forms (cortisol corticosterone). In mammals, two 11β-hydroxysteroid dehydrogenases (11β-HSD1 11β-HSD2) interconvert glucocorticoids, allowing tissue-specific regulation...
Biological aging research is expected to reveal modifiable molecular mechanisms that can be harnessed slow or possibly reverse unhealthy trajectories. However, there first an urgent need define consensus markers of healthy and aging. Established hallmarks are all linked metabolism, a ‘rewired’ metabolic circuitry has been shown accelerate delay biological To identify signatures distinguishing from trajectories, we performed nontargeted metabolomics on skeletal muscles 2-month-old...
Naturally occurring substances are valuable resources for drug development. In this respect, chalcones known to be antiproliferative agents against prostate cancer cell lines through various mechanisms or targets. Based on the literature and preliminary results, we aimed study optimise efficiency of a series inhibit androgen-converting AKR1C3, promote cancer. A total 12 with different substitution patterns were synthesised. Structure-activity relationships associated these modifications...
Differentiation of preadipocytes into mature adipocytes is a highly complex cellular process. At lipidome level, the adipogenesis remains poorly characterized. To investigate lipidomic changes during human adipogenesis, we used LipidyzerTM assay, which quantified 743 lipid species from 11 classes. The undifferentiated SGBS cell strain showed heterogeneous class composition with most abundant classes, phosphatidylethanolamines (PE), phosphatidylcholines (PC), and sphingomyelins (SM)....
Technological advances have brought a steady increase in the availability of various types omics data, from genomics to metabolomics. Integrating these multi-omics data is chance and challenge for systems biology; yet, tools fully tap their potential remain scarce. We present here unsupervised versatile correlation-based method – termed Correlation guided Network Integration (CoNI) integrate into hypergraph structure that allows identification effective modulators metabolism. Our approach...
<h3>Introduction/Background</h3> Endometrial carcinoma (EC) is the most common gynaecological malignancy in developed world. Currently, no valid non-invasive diagnostic or prognostic methods exist, making diagnosis and treatment rely on histopathological surgical findings. The clinical study 'Biomarkers for Diagnosis Prognosis of Carcinoma' (BioEndoCar; NCT03553589) addresses this issue. <h3>Methodology</h3> A prospective observational case-control was conducted at six medical centres across...