A5074429279- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Mycotoxins in Agriculture and Food
- Asthma and respiratory diseases
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Respiratory viral infections research
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Respiratory Support and Mechanisms
- Infection Control and Ventilation
- Pneumonia and Respiratory Infections
- Gene expression and cancer classification
- Precipitation Measurement and Analysis
- Bacterial Genetics and Biotechnology
- COVID-19 and Mental Health
- Pediatric health and respiratory diseases
- Biofuel production and bioconversion
- Respiratory and Cough-Related Research
- Evolution and Genetic Dynamics
- SARS-CoV-2 detection and testing
- Helicobacter pylori-related gastroenterology studies
Helmholtz Zentrum München
2017-2024
German Center for Lung Research
2022-2024
Center for Environmental Health
2023
Institute for Asthma and Allergy
2023
University of Groningen
2014-2019
TiFN
2017-2019
Wageningen University & Research
2010-2012
Rationale In adults, personalised asthma treatment targets patients with type 2 (T2)-high and eosinophilic phenotypes. It is unclear whether such classification achievable in children. Objectives To define T2-high easily accessible biomarkers compare resulting phenotypes across all ages. Methods the multicentre clinical All Age Asthma Cohort (ALLIANCE), 1125 participants (n=776 asthmatics, n=349 controls) were recruited followed for years (1 year adults). Extensive characterisation...
Following lockdown periods and restricting public health measures in response to the COVID-19 pandemic, respiratory tract infections (RTIs) rose significantly worldwide. This led an increased burden on children's hospitals compromising medical care of acutely chronically ill children. We characterized changes epidemiological pattern circulating viral infections.
Wheat has been domesticated into a large number of agricultural environments and the ability to adapt diverse environments. To understand this process, we survey genotype, repeat content, DNA methylation across bread wheat landrace collection representing global genetic diversity. We identify independent variation in methylation, transposon copy number. show that these, so far unexploited, sources have had significant impact on genome ancestral states become preferentially “hard coded” as...
Aspergillus niger is an important organism for the production of industrial enzymes such as hemicellulases and pectinases. The xylan-backbone monomer, d-xylose, inducing substance coordinate expression a large number polysaccharide-degrading enzymes. In this study, responses 22 genes to low (1 mM) high (50 d-xylose concentrations were investigated. These encode that function xylan backbone-degrading enzymes, accessory cellulose-degrading or involved in pentose catabolic pathway A. niger....
Abstract Background Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Resistance to FHB quantitatively controlled by the combined effects many small medium effect QTL. Flowering traits, especially extent extruded anthers, are strongly associated with resistance. Results To characterize genetic basis resistance, we generated and analyzed phenotypic gene expression data on response graminearum ( Fg ) infection in 96 European winter genotypes, including several lines...
ABSTRACT Spore heat resistance, germination, and outgrowth are problematic bacterial properties compromising food safety quality. Large interstrain variation in these makes prediction control of spore behavior challenging. High-level resistance slow germination spores some natural Bacillus subtilis isolates, encountered foods, have been attributed to the occurrence spoVA 2mob operon carried on Tn 1546 transposon. In this study, we further investigate correlation between presence...
Rationale: The strongest genetic risk factor for childhood-onset asthma, the 17q21 locus, is associated with increased viral susceptibility and disease-promoting processes.Objectives: To identify biological targets underlying escalated clinical phenotype mediated by locus.Methods: Genome-wide transcriptome analysis of nasal brush samples from 261 children (78 healthy, 79 wheezing at preschool age, 104 asthmatic) within ALLIANCE (All-Age-Asthma) cohort, a median age 10.0 (range, 1.0–20.0)...
Abstract Introduction Eosinophil‐derived neurotoxin (EDN) is a biomarker for eosinophilic activation. Urinary (u) EDN may allow non‐invasive monitoring of asthma, but clinical recommendations are lacking. We assessed the potential uEDN as marker disease activity in pediatric asthma. Method s urine samples 371 children from German ALLIANCE study cohort, which we had: 169 preschool wheezers (<6 years), 80 asthmatics (≥6 and 122 healthy controls using ImmunoCAP™ Assay. Creatinine...
In this paper the dynamics of transcription-translation system for XlnR regulon in Aspergillus niger is modeled. The model based on Hill regulation functions and uses ordinary differential equations. network response to a trigger D-xylose considered stability analysis performed. activating, repressive feedback, combined effect two feedbacks behavior are analyzed. Simulation systems showed significant influence activating repressing feedback metabolite expression profiles. input function has...
Background There is currently a dramatic increase in the number of COVID-19 cases worldwide, and further drastic restrictions our daily life will be necessary to contain this pandemic. The implications restrictive measures like social-distancing mouth-nose protection on patients with chronic respiratory diseases have hardly been investigated. Methods Our survey, was conducted within All Age Asthma Cohort (ALLIANCE), multicenter longitudinal observational study. We assessed effects imposed...
Respiratory syncytial virus (RSV) is one of the major causes acute lower respiratory tract infections (ALRTI) in children. Children younger than 1 year are most susceptible to RSV infection. occur seasonally temperate climate regions. Based on surveillance and climatic data, we developed statistical models that were assessed compared predict relationship between weather incidence among refugee children 5 years Dadaab camp Kenya. Most time-series analyses rely assumption Gaussian-distributed...
Sporulation is a survival strategy, adapted by bacterial cells in response to harsh environmental adversities. The adaptation potential differs between strains and the variations may arise from differences gene regulation. Gene networks are valuable way of studying such regulation processes establishing associations genes. We reconstructed compared sporulation co-expression (GCNs) model laboratory strain Bacillus subtilis 168 food-borne industrial isolate amyloliquefaciens. Transcriptome...
One of the challenges in genetic network reconstruction is finding experimental designs that maximize information content a data set. In this paper, value mRNA transcription time course experiments was used to compare designs. The study concerns dynamic response genes XlnR regulon Aspergillus niger, with goal find best moment administer an extra pulse inducing D-xylose. Low and high D-xylose pulses were perturb regulon. Evaluation methods based on simulation Models govern regulation target...
Abstract Introduction Following lockdown periods and restricting public health measures in response to the COVID-19 pandemic, respiratory tract infections (RTIs) rose significantly worldwide. This led an increased burden on children's hospitals compromising medical care of acutely chronically ill children. We characterized changes epidemiological pattern circulating viral infections. Methods assessed number patients with RTIs annual distribution virus detections between 2019 2022 based 4809...
Lactic acid bacteria are Gram-positive used throughout the world in many industrial applications for their acidification, flavor and texture formation attributes. One of species, Lactococcus lactis, is employed production fermented milk products like cheese, buttermilk quark. It ferments lactose to lactic and, thus, helps improve shelf life products. Many physiological transcriptome studies have been performed L. lactis order comprehend its biotechnological assets. Using large amounts data...
Transcription of genes coding for xylanolytic and cellulolytic enzymes in Aspergillus niger is controlled by the transactivator XlnR. In this work we analyse model transcription dynamics XlnR regulon from time-course data messenger RNA levels some target genes, obtained reverse quantitative PCR (RT-qPCR). Induction was achieved using low (1 mM) high (50 concentrations D-xylose (Xyl). We investigated wild type strain (Wt) a mutant with partial loss-of-function carbon catabolite repressor CreA...
Abstract Wheat has been domesticated into a large number of agricultural environments and remarkable ability to adapt diverse environments. To understand this process, we survey genotype, repeat content DNA methylation across bread wheat landrace collection representing global genetic diversity. We identify independent variation in methylation, genotype transposon copy number. show that these, so far unexploited, sources have had massive impact on the genome ancestral states become...
Parameter estimation is an important part of computational systems biology – especially in studies on biological networks. Numerous stochastic search methods have been applied parameter In this paper, a constrained space (CSSS) method for proposed and evaluated estimating the parameters genetic network described by differential equations. Both linear nonlinear model formalisms were used data evaluation. The performance CSSS was compared to Integrated Controlled Random Search Dynamic Systems...
Keeping track of the human population is essential for proper planning facilities such as healthcare, infrastructure, education, and other needs. There are various ways by which government can ensure that service provision improved maintained its citizens very often this starts knowing changes in demography a function time.In work mathematical modeling simulations used to study dynamics Uganda. The models prediction county’s how time. Parameter sensitivity analysis was performed using census...