A5009537805- Asthma and respiratory diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Bacteriophages and microbial interactions
- IL-33, ST2, and ILC Pathways
- Respiratory and Cough-Related Research
- Electrowetting and Microfluidic Technologies
- Sarcoma Diagnosis and Treatment
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pediatric health and respiratory diseases
- SARS-CoV-2 detection and testing
- Inhalation and Respiratory Drug Delivery
- Biosensors and Analytical Detection
- Immune cells in cancer
- Vitamin C and Antioxidants Research
Philipps University of Marburg
2015-2024
Loewe Center for Synthetic Microbiology
2022-2024
Abstract Rationale and Objective Plasma extracellular vesicles (EVs) represent a vital source of molecular information about health disease states. Due to their heterogenous cellular sources, EVs cargo may predict specific pathomechanisms behind phenotypes. Here we aimed utilize EV microRNA (miRNA) signatures gain new insights into underlying mechanisms obesity‐associated low type‐2 asthma. Methods Obese asthma (OA) non‐obese (NOA) patients were selected from an cohort conjointly with...
Extracellular vesicles (EVs) are released by virtually all cells and may serve as intercellular communication structures transmitting molecules such proteins, lipids, nucleic acids between cells. MicroRNAs (miRNAs) an abundant class of vesicular RNA playing a pivotal role in regulating intracellular processes. In this work, we aimed to characterize miRNA profiles side-directed manner bronchial epithelial from healthy asthmatic subjects using air-liquid interface cell culture model. EVs were...
Abstract Introduction Eosinophil‐derived neurotoxin (EDN) is a biomarker for eosinophilic activation. Urinary (u) EDN may allow non‐invasive monitoring of asthma, but clinical recommendations are lacking. We assessed the potential uEDN as marker disease activity in pediatric asthma. Method s urine samples 371 children from German ALLIANCE study cohort, which we had: 169 preschool wheezers (<6 years), 80 asthmatics (≥6 and 122 healthy controls using ImmunoCAP™ Assay. Creatinine...
The honeybee (Apis mellifera) represents a model organism for social insects displaying behavioral plasticity. This is reflected by an age-dependent task allocation. most protruding tasks are performed young nurse bees and older forager that take care of the brood inside hive collect food from outside hive, respectively. molecular mechanism leading to transition foragers currently under intense research. Circular RNAs, however, were not considered in this context so far. As today, group...
In the era of personalized medicine, insights into molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); non-obese, normal BMI (23.88 2.73); and healthy controls (23.62 3.74). All patients were considered represent low type-2 phenotype according selective clinical...
Background: Increasing knowledge of cancer biology and an expanding spectrum molecularly targeted therapies provide the basis for precision oncology. Despite extensive gene diagnostics, previous reports indicate that less than 10% patients benefit from this concept. Methods: We retrospectively analyzed all referred to our center’s Molecular Tumor Board (MTB) 2018 2021. testing by next-generation sequencing (NGS) included a 67-gene panel detection short-sequence variants copy-number...
The ATP-dependent nucleosome remodeler Mi-2/CHD4 broadly modulates chromatin landscapes to repress transcription and maintain genome integrity. Here we use individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) show that Drosophila Mi-2 associates with thousands of mRNA molecules in vivo. Biochemical data reveal recombinant dMi-2 preferentially binds G-rich RNA using two intrinsically disordered regions unclear function. Pharmacological inhibition RNase digestion...
Noncoding 6S RNAs regulate transcription by binding to the active site of bacterial RNA polymerase holoenzymes. Processing and decay 6S-1 6S-2 were investigated in Bacillus subtilis northern blot RNA-seq analyses using different RNase knockout strains, as well vitro processing assays. For both paralogs, we identified a key—but mechanistically different—role J1. J1 catalyzes 5′-end maturation RNA, yet relatively inefficient possibly via enzyme's “sliding endonuclease” activity. has no...
Optimal pre-analytical conditions for blood sample processing and isolation of selected cell populations subsequent transcriptomic epigenomic studies are required to obtain robust reproducible results. This pilot study was conducted investigate the potential effects timing CD4+ T-cell from peripheral atopic non-atopic adults on their epigenetic profiles. Two heparinized samples were drawn each three healthy individuals. For individual, T-cells isolated first within 2 h (immediate) or second...
Plasma extracellular vesicles (EVs) and their cargo represent a vital source of molecular information about health disease states may predict specific pathomechanisms behind phenotypes. Here we aimed to utilize EV microRNA (miRNA) signatures gain new insights into underlying mechanisms obesity-associated low type-2 asthma. Ten obese (OA) ten non-obese asthma (NOA) patients were selected from an cohort alongside with healthy controls. EVs isolated characterised by nanoparticle tracking...
<b>Background:</b> Eosinophil-derived neurotoxin (EDN) is a biomarker for eosinophilic inflammation. Serum EDN and urinary (uEDN) concentrations are higher among asthmatics. uEDN non-invasive marker inflammation that could support early asthma diagnosis (wheezing <6 years). Clinical guidelines this lacking. <b>Aim:</b> Examine uEDN, potential wheeze/asthma diagnosis, disease severity, phenotype endotypes. <b>Methods:</b> We statistically analyzed of 219 children: n=49 wheeze; n=62 asthma;...
The ATP-dependent nucleosome remodeller Mi-2/CHD4 broadly modulates epigenetic landscapes to repress transcription and maintain genome integrity. Here we use individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) show that Drosophila Mi-2 associates with thousands of mRNA molecules in vivo. Biochemical data reveal recombinant dMi-2 preferentially binds G-rich RNA using two intrinsically disordered regions previously undefined function. Pharmacological inhibition RNase...