A5088925749- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Chromatin Remodeling and Cancer
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- Virus-based gene therapy research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- Neurobiology and Insect Physiology Research
- FOXO transcription factor regulation
- Protein Tyrosine Phosphatases
- NF-κB Signaling Pathways
- Animal Genetics and Reproduction
- interferon and immune responses
- Acute Ischemic Stroke Management
Philipps University of Marburg
2008-2023
Institute of Molecular Biology
2017-2022
Osnabrück University
2022
Helmholtz Centre for Infection Research
2012
German Cancer Research Center
2010
Heidelberg University
2010
Ludwig-Maximilians-Universität München
2000-2005
Harvard University
2004
Massachusetts General Hospital
2004
Center for Cancer Research
2004
ABSTRACT The totipotential stem cells of the pregastrulation mouse embryo which give rise to all embryonic somatic tissues and germ express Oct-4. expression is downregulated during gastrulation thereafter only maintained in germline lineage. Oct-4/lacZ transgenes were used determine how this pattern was achieved, resulted identification two separate regulatory elements. distal element drives Oct-4 preimplantation embryos, migratory postmigratory primordial but inactive epiblast. In cell...
Hydroxymethylcytosine, well described in DNA, occurs also RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing transcripts of many genes, notably coding sequences, identify consensus sites for hydroxymethylation. found RNA can favor mRNA translation. hydroxymethylated are to be most abundant Drosophila brain, Tet-deficient fruitflies suffer impaired...
International guidelines recommend avoiding intravenous thrombolysis (IVT) in patients with ischemic stroke who have a recent intake of direct oral anticoagulant (DOAC). To determine the risk symptomatic intracranial hemorrhage (sICH) associated use IVT DOAC ingestion. This international, multicenter, retrospective cohort study included 64 primary and comprehensive centers across Europe, Asia, Australia, New Zealand. Consecutive adult received (both without thrombectomy) were included....
Here we report the identification of LIN complex (LINC), a human multiprotein that is required for transcriptional activation G2/sub>/M genes. LINC related to recently identified dREAM and DRM complexes Drosophila C.elegans contain homologues mammalian retinoblastoma tumor suppressor protein. The core consists at least five subunits including chromatin-associated LIN-9 RbAp48 proteins. dynamically associates with pocket proteins, E2F B-MYB during cell cycle. In quiescent cells, binds p130...
AbstractThe transcriptional status of eukaryotic genes is determined by a balance between activation and repression mechanisms. The nuclear hormone receptors represent classical examples transcription factors that can regulate this recruiting corepressor coactivator complexes in ligand-dependent manner. Here, we demonstrate the equilibrium via single factor, Elk-1, altered following Erk mitogen-activated protein kinase cascade. In addition to its C-terminal domain, Elk-1 contains an...
Eukaryotic cells respond to genomic and environmental stresses, such as DNA damage heat shock (HS), with the synthesis of poly-[ADP-ribose] (PAR) at specific chromatin regions, breaks or HS genes, by PAR polymerases (PARP). Little is known about role this modification during cellular stress responses. We show here that nucleosome remodeler dMi-2 recruited active genes in a PARP–dependent manner. binds suggesting physical interaction important for recruitment. Indeed, mutant unable bind does...
The POU transcription factor Oct-4 is expressed in totipotent and pluripotent cells of the early mouse embryo germ cell lineage. Transactivation capacities regions flanking DNA binding domain were analyzed undifferentiated differentiated lines. amino- carboxy-terminal (N C domain) fused to Gal4 both functioned as transactivation domains all lines tested. However, failed activate some context native protein. underlying regulatory mechanism appears involve can discriminate between different...
Chromosome structure, DNA metabolic processes and cell type identity can all be affected by changing the positions of nucleosomes along chromosomal DNA, a reaction that is catalysed SNF2-type ATP-driven chromatin remodelers. Recently it was suggested in vivo, more than 50% nucleosome predicted simply sequence, especially within promoter regions. This seemingly contrasts with remodeler induced mobility. The ability remodeling enzymes to mobilise over short distances well documented. However,...