A5034032941- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related gene regulation
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Chromatin Remodeling and Cancer
- Insect Resistance and Genetics
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Genetics, Bioinformatics, and Biomedical Research
- Plant Molecular Biology Research
- Microtubule and mitosis dynamics
- Heat shock proteins research
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Estrogen and related hormone effects
- Molecular Biology Techniques and Applications
Ludwig-Maximilians-Universität München
2016-2025
Urologische Klinik München
2019-2023
Center for Integrated Protein Science Munich
2010-2019
Novo Nordisk (Denmark)
2007-2016
University of Birmingham
2007
University of Cambridge
2004
European Molecular Biology Laboratory
1993-2003
European Bioinformatics Institute
1997-2001
Universitäts Frauenklinik
2000
Biologie Labor
2000
Dosage compensation in Drosophila involves a 2-fold increase transcription from the single male X relative to two female chromosomes. Regulation at level of chromosome alterations chromatin organization: chromosomes appear decondensed and are marked by acetylation histone H4 lysine 16. We demonstrate that MOF, protein required for dosage with significant sequence similarity MYST family acetyltransferases, is acetyltransferase acetylates specifically This relieves chromatin-mediated...
Using in vivo dimethylsulfate footprinting, we have analyzed protein-DNA interactions within two regions upstream of the tyrosine aminotransferase (TAT) gene that are characterized by an altered chromatin structure TAT-expressing as compared to nonexpressing cells. All identified protein contacts DNA found exclusively hepatoma In vitro analyses specific DNA-binding factors crude nuclear extracts yield DNAase I footprints correlate well with binding sites vivo. Surprisingly, all activities...
Abstract The basic helix–loop–helix (bHLH) family of transcription factors recognizes DNA motifs known as E-boxes (CANNTG) and includes 108 members 1 . Here we investigate how chromatinized are engaged by two structurally diverse bHLH proteins: the proto-oncogene MYC-MAX circadian factor CLOCK-BMAL1 (refs. 2,3 ). Both bind to preferentially near nucleosomal entry–exit sites. Structural studies with engineered or native nucleosome sequences show that triggers release from histones gain...
The chromatin accessibility complex (CHRAC) belongs to the class of nucleosome remodeling factors that increase nucleosomal DNA in an ATP-dependent manner. We found CHRAC induces movements intact histone octamers neighboring segments without facilitating their displacement competing or chaperones trans. CHRAC-induced energy-dependent sliding may, principle, explain remodeling, positioning, and spacing reactions known be catalyzed by CHRAC. catalytic core CHRAC, ATPase ISWI, also mobilized...
Sensing DNA damage is crucial for the maintenance of genomic integrity and cell cycle progression. The participation chromatin in these events becoming increasing interest. We show that presence single-strand breaks gaps, formed either directly or during processing, can trigger propagation nucleosomal arrays. This nucleosome assembly pathway involves histone chaperone factor 1 (CAF-1). largest subunit (p150) this interacts with proliferating nuclear antigen (PCNA), critical regions...
The ATPase ISWI is a subunit of several distinct nucleosome remodeling complexes that increase the accessibility DNA in chromatin. We found isolated protein itself was able to carry out remodeling, rearrangement, and chromatin assembly reactions. activity stimulated by nucleosomes but not free or histones, indicating recognizes specific structural feature nucleosomes. Nucleosome therefore, does require functional interaction between other subunits complexes. role proteins associated with may...
The ATPase ISWI can be considered the catalytic core of several multiprotein nucleosome remodeling machines.Alone or in context factor, chromatin accessibility complex (CHRAC), ACF, catalyzes a number ATP-dependent transitions structure that are currently best explained by its ability to induce sliding.In addition, function as spacing factor during assembly, where it will trigger ordering newly assembled nucleosomes into regular arrays.Both and reactions mechanistically unexplained.As step...
We describe a cell-free system, derived from preblastoderm Drosophila embryos, for the efficient assembly of cloned DNA into chromatin. The chromatin system utilizes endogenous core histones and factors yields long arrays regularly spaced nucleosomes with repeat length 180 bp. is also capable complementary-strand synthesis accompanied by rapid nucleosome formation when starting template single-stranded circular DNA. Chromatin assembled embryo extract naturally deficient in histone H1, but...