A5073962862- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Plant nutrient uptake and metabolism
- Cancer-related gene regulation
- Melanoma and MAPK Pathways
- Genomics and Phylogenetic Studies
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 detection and testing
- Plant responses to water stress
- Plant Gene Expression Analysis
- Virus-based gene therapy research
- Seed Germination and Physiology
- Bioenergy crop production and management
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Asthma and respiratory diseases
- Immune responses and vaccinations
- Molecular Biology Techniques and Applications
Direction de la Recherche Fondamentale
2017-2024
Centre National de Recherche en Génomique Humaine
2017-2024
Université Paris-Saclay
2021-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2024
CEA Paris-Saclay
2021-2024
Cornell University
2017-2019
Weill Cornell Medicine
2017
Genoscope
2010-2015
CEA Grenoble
2015
CEA Cadarache
2015
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and oleracea, followed chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms high gene content. We examined consequences its recent duplication. The constituent An Cn subgenomes are engaged in subtle structural, functional, epigenetic cross-talk, abundant homeologous...
MicroRNAs (miRNAs) play an important role in the pathogenesis of autoimmune diseases such as primary Sjögren's syndrome (pSS). This study is first to investigate miRNA expression patterns purified T and B lymphocytes from patients with pSS using a high-throughput quantitative PCR (qPCR) approach.Two independent cohorts both controls, one for discovery replication, were included this study. CD4+ cells CD19+ isolated peripheral blood mononuclear by magnetic microbeads miRNAs was profiled...
Abstract Rationale and Objective Plasma extracellular vesicles (EVs) represent a vital source of molecular information about health disease states. Due to their heterogenous cellular sources, EVs cargo may predict specific pathomechanisms behind phenotypes. Here we aimed utilize EV microRNA (miRNA) signatures gain new insights into underlying mechanisms obesity‐associated low type‐2 asthma. Methods Obese asthma (OA) non‐obese (NOA) patients were selected from an cohort conjointly with...
Epicutaneous immunotherapy (EPIT) is a promising method for treating food allergies. In animal models, EPIT induces sustained unresponsiveness and prevents further sensitization mediated by Tregs. Here, we elucidate the mechanisms underlying therapeutic effect of EPIT, characterizing kinetics DNA methylation changes in sorted cells from spleen blood evaluating its persistence bystander compared to oral (OIT).BALB/c mice orally sensitized peanut proteins (PPE) were treated using PPE-patch or...
Trees are long-lived organisms that continuously adapt to their environments, a process in which epigenetic mechanisms likely play key role. Via downregulation of the chromatin remodeler DECREASED IN DNA METHYLATION 1 (DDM1) poplar (Populus tremula × Populus alba) RNAi lines, we examined how methylation coordinates genomic and physiological responses moderate water deficit. We compared growth drought response two RNAi-ddm1 lines wild-type (WT) trees under well-watered deficit/rewatering...
Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level expression chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1) two demethylases DEMETER-LIKE (DML) in Populus tremula × alba lines, we examined how host methylation modulates multiple parameters root colonization with fungus Laccaria bicolor. We compared ectomycorrhizas formed...
Breast cancer is a heterogeneous disease for which alterations in DNA methylation patterns have been shown to be of biological and clinical importance. Here we report on the integrated analysis molecular including status 27 gene promoters analyzed by highly quantitative pyrosequencing, association expression, germline genotype parameters survival. specific deregulation (both hyper- hypomethylation) was found twenty genes ACVR1, OGG1, IL8 TFF1. The level promoter regions significantly...
Catharanthus roseus produces a wide spectrum of monoterpene indole alkaloids (MIAs). MIA biosynthesis requires tightly coordinated pathway involving more than 30 enzymatic steps that are spatio-temporally and environmentally regulated so some MIAs specifically accumulate in restricted plant parts. The first regulatory layer involves complex network transcription factors from the basic Helix Loop (bHLH) or AP2 families. In present manuscript, we investigated whether an additional epigenetic...
Abstract Background Early‐life exposure to certain environmental bacteria including Acinetobacter lwoffii ( AL ) has been implicated in protection from chronic inflammatory diseases asthma later life. However, the underlying mechanisms at immune‐microbe interface remain largely unknown. Methods The effects of repeated intranasal on local and systemic innate immune responses were investigated wild‐type Il6 −/− , Il10 Il17 mice exposed ovalbumin‐induced allergic airway inflammation. Those...
Abstract The COVID-19 pandemic caused by the new Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) continues to threaten public health and burden healthcare systems worldwide. Whole SARS-CoV-2 genome sequencing has become essential for epidemiological monitoring identification of variants, which could represent a risk increased transmissibility, virulence, or resistance vaccines treatment. Different next-generation approaches are used in sequencing, although with different ability...
We report noggin mutations in three Spanish families with fibrodysplasia ossificans progressiva (FOP). The propositi have typical FOP findings; the first and third parents are unaffected, while second family father is partially affected. DNA of their was screened by sequencing for gene (NOG). Sequencing indicated a G to C mutation at nucleotide 274 NOG propositus, encoding G92R substitution peptide level; this de novo, corresponding change not being observed parents. In T 271 encodes G91C...
SUMMARY How perennial organisms adapt to environments is a key question in biology. To address this question, we investigated ten natural black poplar ( Populus nigra ) populations from Western Europe, keystone forest tree of riparian ecosystems. We assessed the role (epi)genetic regulation driving species evolution and adaptation over several millions years (macro-evolution) up few generations (micro-evolution). At macro-evolution scale, polar experienced differential structural (gene loss)...
Wastewater-based epidemiology is experiencing exponential development. Despite undeniable advantages compared to patient-centered approaches (cost, anonymity, survey of large populations without bias, detection asymptomatic infected peoples…), major technical limitations persist. Among them the low sensitivity current methods used for quantifying and sequencing viral genomes from wastewater. In situations circulation, during initial stages emergences, or in areas heavy rains, extremely...
Summary In a context of climate change, it is necessary to decipher the strategies established by plants cope with limited water supply. Transcriptome, methylome and small RNA data were generated for two oak species contrasting levels drought tolerance ( Quercus robur petraea ), under control stress conditions All are in line species-specific response consistent their ecological preferences. The biological processes associated genomic regions identified all datasets mainly parietal Q....
Abstract As fixed and long living organisms subjected to repeated environmental stresses, trees have developed mechanisms such as phenotypic plasticity that help them cope with fluctuating conditions. Here, we tested the role DNA methylation a hub of integration, linking physiological response water deficit in shoot apical meristem model tree poplar ( Populus ). Using reverse genetic approach, compared hypomethylated RNAi- ddm1 lines wild-type for drought tolerance. An integrative analysis...
Abstract Embryogenesis is a brief but potentially critical phase in the tree life cycle for adaptive phenotypic plasticity. Using somatic embryogenesis maritime pine, we found that temperature during maturation affects embryo development and post-embryonic growth up to three years. We examined whether this stress memory could stem from temperature- and/or development-induced changes DNA methylation. To do this, developed 200 Mb custom sequence capture bisulfite analysis of genes promoters...
Abstract Embryogenesis is a brief but potentially critical phase in the life cycle of tree for adaptive phenotypic plasticity. Using somatic embryogenesis maritime pine (Pinus pinaster Ait.), we found that temperature during maturation affects embryo development and postembryonic growth up to 3 yr. We examined whether this stress memory could stem from temperature- and/or development-induced changes DNA methylation. For this, developed 200 mb custom sequence capture bisulfite analysis genes...