- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Wheat and Barley Genetics and Pathology
- Plant Reproductive Biology
- Genomics and Chromatin Dynamics
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Agriculture and Rural Development Research
- Light effects on plants
- Genetic Mapping and Diversity in Plants and Animals
- Celiac Disease Research and Management
- CRISPR and Genetic Engineering
- Nitrogen and Sulfur Effects on Brassica
- Lipid metabolism and biosynthesis
Institut de Biologie de l'École Normale Supérieure
2015-2019
Université Paris Sciences et Lettres
2018-2019
Centre National de la Recherche Scientifique
2010-2019
Inserm
2013-2019
Université d'Évry Val-d'Essonne
2014
Université Clermont Auvergne
2013
Genetique Reproduction and Developpement
2013
Clermont Université
2013
Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères
2012
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and oleracea, followed chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms high gene content. We examined consequences its recent duplication. The constituent An Cn subgenomes are engaged in subtle structural, functional, epigenetic cross-talk, abundant homeologous...
The spatial organization of chromatin can be subject to extensive remodeling in plant somatic cells response developmental and environmental signals. However, the mechanisms controlling these dynamic changes their functional impact on nuclear activity are poorly understood. Here, we determined that light perception triggers a switch between two different architectural schemes during Arabidopsis postembryonic development. Whereas progressive nucleus expansion heterochromatin rearrangements...
Chromatin provides a tunable platform for gene expression control. Besides the well-studied core nucleosome, H1 linker histones are abundant chromatin components with intrinsic potential to influence function. Well studied in animals, little is known about evolution of function other eukaryotic lineages instance plants. Notably, model plant Arabidopsis, while heterochromatin and DNA methylation, its contribution transcription, molecular, cytological organization remains elusive. We provide...
• To understand key mechanisms leading to stabilized allopolyploid species, we characterized the meiotic behaviour of wheat allohexaploids in relation structural and genetic changes. For that purpose, analysed first generations synthetic obtained through interspecific hybridization, followed by spontaneous chromosome doubling, between several genotypes Triticum turgidum Aegilops tauschii donors AB D genomes, respectively. As expected for these Ph1 (Pairing homoeologous 1) gene-carrying...
• The present study aims to understand regulation of gene expression in synthetic and natural wheat (Triticum aestivum) allohexaploids, that combines the AB genome Triticum turgidum D Aegilops tauschii; which we have recently characterized as genetically stable. We conducted a comprehensive genome-wide analysis allowed characterization effect variability progenitor, intergenerational stability well comparison with allohexaploid. used Affymetrix GeneChip Wheat Genome Array, on 55 049...
Summary The reprogramming of gene expression appears as the major trend in synthetic and natural allopolyploids where an important proportion genes was shown to deviate from that parents or average parents. In this study, we analyzed changes previously reported, highly stable wheat allohexaploids combine D genome Aegilops tauschii AB extracted hexaploid Triticum aestivum . A comprehensive genome‐wide analysis transcriptional using Affymetrix GeneChip Wheat Genome Array conducted. Prevalence...
Summary Using floral‐dip, tumorigenesis and root callus transformation assays of both germline somatic cells, we present here results implicating the four major non‐homologous homologous recombination pathways in Agrobacterium ‐mediated A rabidopsis thaliana . All single mutant lines showed similar mild reductions transformability, but knocking out three severely compromised grobacterium transformation. Although integration T– DNA into plant genome is absence known double‐strand break repair...
Abstract Chromatin in eukaryotes provides a tunable platform to control gene expression and convey an epigenetic memory throughout cell divisions. H1 linker histones are abundant components with intrinsic potential influencing chromatin structure function. We detail the impact of depletion Arabidopsis on fine-scale organization, transcription development. While required for chromocenter assembly, H1s dispensable transposable element (TE) silencing peripheral positioning heterochromatin. In...