A5084102552- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Wheat and Barley Genetics and Pathology
- Plant Reproductive Biology
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Plant Taxonomy and Phylogenetics
- Plant Pathogens and Resistance
- Banana Cultivation and Research
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Botanical Research and Chemistry
- Animal Genetics and Reproduction
- Plant nutrient uptake and metabolism
- Nitrogen and Sulfur Effects on Brassica
- Plant Physiology and Cultivation Studies
- Plant and fungal interactions
- Algal biology and biofuel production
- Plant chemical constituents analysis
- Horticultural and Viticultural Research
- Aquaculture Nutrition and Growth
Institut de Génétique, Environnement et Protection des Plantes
2015-2024
Université de Rennes
2009-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
L'Institut Agro
2020-2024
Institut Agro Rennes-Angers
2006-2020
Département Biologie et Amélioration des Plantes
2004-2019
Institut National de la Recherche Agronomique du Niger
2017
Institut National de la Recherche Agronomique
2006-2014
Département Santé des Plantes et Environnement
2010-2011
Centre National de la Recherche Scientifique
1998-2000
• Polyploidy promotes the restructuring of merged genomes within initial generations resynthesized Brassica napus, possibly caused by homoeologous recombination at meiosis. However, little is known about impact first confrontation two meiosis which could lead to genome exchanges in progeny. Here, we assessed role instability synthetic B. napus. We used three different newly napus plants and established meiotic pairing frequencies for A C genomes. genotyped corresponding progenies a cross...
Endopolyploidy is a widespread process that corresponds to the amplification of genome in absence mitosis. In tomato, very high ploidy levels (up 256C) are reached during fruit development, concomitant with large cell sizes. Using cellular approaches (fluorescence and electron microscopy) we provide structural analysis endoreduplicated nuclei at level chromatin nucleolar organisation, nuclear shape relationship other organelles such as mitochondria. We demonstrate endopolyploidy pericarp...
Genomic rearrangements arising during polyploidization are an important source of genetic and phenotypic variation in the recent allopolyploid crop Brassica napus. Exchanges among homoeologous chromosomes, due to interhomoeologue pairing, deletions without compensating duplications observed both natural B. napus synthetic Rearrangements large or small chromosome segments induce gene copy number (CNV) can potentially cause changes. Unfortunately, complex genome restructuring is difficult deal...
Chromosomal rearrangements can be triggered by recombination between distinct but related regions. Brassica napus (AACC; 2n = 38) is a recent allopolyploid species whose progenitor genomes are widely replicated. In this article, we analyze the extent to which chromosomal originate from homeologous during meiosis of haploid B. (n 19) genotyping progenies x euploid with molecular markers. Our study focuses on three pairs regions selected for their differing levels divergence (N1/N11, N3/N13,...
Abstract Transposable elements (TEs) constitute >80% of the wheat genome but their dynamics and contribution to size variation evolution genomes (Triticum Aegilops species) remain unexplored. In this study, 10 genomic regions have been sequenced from chromosome 3B used constitute, along with all publicly available sequences wheat, 1.98 Mb sequence (from 13 BAC clones) B 3.63 19 A genome. Analysis TE proportions (as percentages), ratios complete truncated copies, estimation insertion...
• The present study aims to understand regulation of gene expression in synthetic and natural wheat (Triticum aestivum) allohexaploids, that combines the AB genome Triticum turgidum D Aegilops tauschii; which we have recently characterized as genetically stable. We conducted a comprehensive genome-wide analysis allowed characterization effect variability progenitor, intergenerational stability well comparison with allohexaploid. used Affymetrix GeneChip Wheat Genome Array, on 55 049...
Meiotic recombination is the fundamental process that produces balanced gametes and generates diversity within species. For successful meiosis, crossovers must form between homologous chromosomes. This condition more difficult to fulfill in allopolyploid species, which have than two sets of related chromosomes (homoeologs). Here, we investigated formation, progression, completion several key hallmarks meiosis Brassica napus (AACC), a young polyphyletic allotetraploid crop species with...
Abstract Meiotic crossovers are necessary to generate balanced gametes and increase genetic diversity. Even if crossover number is usually constrained, recent results suggest that manipulating karyotype composition could be a new way frequency in plants. In this study, we explored hypothesis by analyzing the extent of variation set related diploid AA, allotriploid AAC, allotetraploid AACC Brassica hybrids. We first used cytogenetic methods describe meiotic behavior different then combined...
Summary Meiotic recombination is a major evolutionary process generating genetic diversity at each generation in sexual organisms. However, this highly regulated, with the majority of crossovers lying distal chromosomal regions that harbor low DNA methylation levels. Even these regions, some islands without remain, for which we investigated underlying causes. Genetic maps were established two Brassica napus hybrids to detect presence such large nonrecombinant islands. The role played by and...
Abstract The tight regulation of meiotic recombination between homologs is disrupted in Brassica AAC allotriploids, a genomic configuration that may have facilitated the formation rapeseed (Brassica napus L.) ∼7,500 years ago. Indeed, presence haploid C genome induces supernumerary crossovers homologous A chromosomes with dramatically reshaped distribution. However, genetic mechanisms driving this phenomenon and their divergence nascent established lineages remain unclear. To address these...
Meiotic recombination by crossovers (COs) is tightly regulated, limiting its key role in producing genetic diversity. However, while COs are usually restricted number and not homogenously distributed along chromosomes, we show here how to disrupt these rules Brassica species using allotriploid hybrids (AAC, 2n = 3x 29), resulting from the cross between allotetraploid rapeseed (B. napus, AACC, 4x 38) one of diploid progenitors rapa, AA, 2x 20). We produced mapping populations different...
Endopolyploidy, i.e. amplification of the genome in absence mitosis, occurs many plant species and happens along with organ cell differentiation. Deciphering functional roles endopolyploidy is hampered by fact that polyploid tissues generally comprise cells various ploidy levels. In some fleshy fruits (amongst them tomato fruit) levels present at end development range from 2C to 256C same tissue. To investigate temporal spatial distribution it necessary address DNA content individual nuclei...
Summary Recombination is a major mechanism generating genetic diversity, but the control of crossover rate remains key question. In Brassica napus ( AACC , 2 n = 38), we can increase homologous recombination between A genomes in AAC hybrids. Hypotheses for this effect include number C univalent chromosomes, ratio univalents and bivalents and, finally, which chromosomes are univalents. To test these hypotheses, produced AA hybrids with zero, one, three, six or nine additional four different...
Summary The reprogramming of gene expression appears as the major trend in synthetic and natural allopolyploids where an important proportion genes was shown to deviate from that parents or average parents. In this study, we analyzed changes previously reported, highly stable wheat allohexaploids combine D genome Aegilops tauschii AB extracted hexaploid Triticum aestivum . A comprehensive genome‐wide analysis transcriptional using Affymetrix GeneChip Wheat Genome Array conducted. Prevalence...
Abstract Production of allohexaploid Brassica (2n = AABBCC) is a promising goal for plant breeders due to the potential hybrid heterosis and useful allelic contributions from all three genomes present in cultivated diploid AA, 2n BB, CC) allotetraploid AABB, AACC, BBCC) crop species (canola, cabbages, mustards). We used high-throughput SNP molecular marker assays, flow cytometry, fluorescent situ hybridization (FISH) characterize population putative allohexaploids derived self-pollination...
Background and aimsBrassica napus (AACC, 2n = 38, oilseed rape) is a relatively recent allotetraploid species derived from the putative progenitor diploid Brassica rapa (AA, 20) oleracea (CC, 18). To determine influence of intensive breeding conditions on evolution its genome, we analysed structure copy number rDNA in 21 cultivars B. napus, representative genetic diversity. Methods We used next-generation sequencing genomic approaches, Southern blot hybridization, expression analysis...
Traditionally, reference genomes in crop species rely on the assembly of one accession, thus occulting most intraspecific diversity. However, rearrangements, gene duplications and transposable element content may have a large impact genomic structure, which could generate new phenotypic traits. Using two Brassica rapa recently sequenced assembled using long-read technology optical mapping, we investigated structural variants repetitive between accessions genome size variation among core...
The genus Ludwigia L. section Jussiaea is composed of a polyploid species complex with 2x, 4x, 6x and 10x ploidy levels, suggesting possible hybrid origins. aim the present study to understand genomic relationships among diploid in Jussiaea. Morphological cytogenetic observations, controlled crosses, situ hybridization (GISH), flow cytometry were used characterize species, patterns, composition across taxa. Genome sizes obtained agreement diploid, tetraploid, hexaploid, decaploid levels....
Agriculture faces great challenges to overcome global warming and improve system sustainability, requiring access novel genetic diversity. So far, wild populations local landraces remain poorly explored. This is notably the case for two diploid species, Brassica oleracea L. (CC, 2n=2x=18) B. rapa (AA, 2n=2x=20). In order explore diversity in both we have collected their centre of origin, Mediterranean basin, on a large contrasting climatic soil gradient from northern Europe southern...
Most banana cultivars are triploid seedless parthenocarpic clones derived from hybridization between Musa acuminata subspecies and sometimes M. balbisiana. were suggested to differ by a few large chromosomal rearrangements based on chromosome pairing configurations in intersubspecies hybrids. We searched for seedy ssp. malaccensis accession through mate-pair sequencing, BAC-FISH, targeted PCR marker (DArTseq) segregation its progeny. identified heterozygous reciprocal translocation involving...
Furanocoumarins are specialized metabolites that involved in the defense of plants against phytophagous insects. The molecular and functional characterization genes their biosynthetic pathway is only partially complete. Many recent reports have described gene clusters responsible for biosynthesis plants. To investigate possible co-localization furanocoumarin pathway, we sequenced parsnip BAC clones spanning two different loci. We found previously identified this CYP71AJ3 CYP71AJ4, were...