A5045229567- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Criminal Law and Evidence
- Polysaccharides and Plant Cell Walls
- Forest Biomass Utilization and Management
- Wheat and Barley Genetics and Pathology
- International Law and Human Rights
- Plant biochemistry and biosynthesis
- Multiculturalism, Politics, Migration, Gender
- Enzyme Catalysis and Immobilization
- European and International Law Studies
- French Urban and Social Studies
- Studies on Chitinases and Chitosanases
- Plant Parasitism and Resistance
- Health, Medicine and Society
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Agriculture and Rural Development Research
- Global Peace and Security Dynamics
- Plant responses to water stress
- Comparative and International Law Studies
Central European Institute of Technology – Masaryk University
2021-2024
University of Exeter
2018-2023
Central European Institute of Technology
2021-2023
Université d'Évry Val-d'Essonne
2012-2017
Pohang University of Science and Technology
2014-2017
Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères
2012
Centre National de la Recherche Scientifique
2012
Institut National de la Recherche Agronomique
2012
National Taiwan University
2012
Centre National de la Propriété Forestière
2004-2007
Insights from the annotated wheat genome Wheat is one of major sources food for much world. However, because bread wheat's a large hybrid mix three separate subgenomes, it has been difficult to produce high-quality reference sequence. Using recent advances in sequencing, International Genome Sequencing Consortium presents an with detailed analysis gene content among subgenomes and structural organization all chromosomes. Examples quantitative trait mapping CRISPR-based modification show...
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and oleracea, followed chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms high gene content. We examined consequences its recent duplication. The constituent An Cn subgenomes are engaged in subtle structural, functional, epigenetic cross-talk, abundant homeologous...
Stomata play an important role in plant innate immunity by limiting pathogen entry into leaves but molecular mechanisms regulating stomatal closure upon perception are not well understood. Here we show that the Arabidopsis thaliana L-type lectin receptor kinase-V.5 (LecRK-V.5) negatively regulates immunity. Loss of LecRK-V.5 function increased resistance to surface inoculation with virulent bacteria Pseudomonas syringae pv tomato DC3000. Levels were affected after infiltration-inoculation,...
Stomata play an important role in preinvasive defense responses by limiting pathogen entry into leaves. Although the stress hormones salicylic acid (SA) and abscisic (ABA) are known to regulate stomatal immunity, of growth promoting is far from understood. Here, we show that Arabidopsis thaliana, cytokinins (CKs) function responses. The cytokinin receptor HISTIDINE KINASE3 (AHK3) RESPONSE REGULATOR2 (ARR2) promote closure triggered pathogen-associated molecular pattern (PAMP) resistance...
Abstract The enzymes involved in l-ascorbate biosynthesis photosynthetic organisms (the Smirnoff–Wheeler [SW] pathway) are well established. Here, we analyzed their subcellular localizations and potential physical interactions assessed role the control of ascorbate synthesis. Transient expression C terminal-tagged fusions SW genes Nicotiana benthamiana Arabidopsis thaliana mutants complemented with genomic constructs showed that while GDP-d-mannose epimerase is cytosolic, all from...
SUMMARY Stomatal defences are important for plants to prevent pathogen entry and further colonisation of leaves. Apoplastic reactive oxygen species (ROS) generated by NADPH oxidases apoplastic peroxidases play an role in activating stomatal closure upon perception bacteria. However, downstream events, particularly the factors influencing cytosolic hydrogen peroxide (H 2 O ) signatures guard cells poorly understood. We used H sensor roGFP2‐Orp1 a ROS‐specific fluorescein probe study...
In Eukaryotes, LIM proteins act as developmental regulators in basic cellular processes such regulating the transcription or organizing cytoskeleton. The domain protein family plants has mainly been studied sunflower and tobacco plants, where several of its members exhibit a specific pattern expression pollen. this paper, we finely characterized poplar six transcripts encoding these proteins. Populus trichocarpa genome, 12 gene models identified all appear to be duplicated genes. addition,...
Abstract Reactive oxygen species are produced in response to pathogens and pathogen-associated molecular patterns, as exemplified by the rapid extracellular oxidative burst dependent on NADPH oxidase isoform RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) Arabidopsis (Arabidopsis thaliana). We used H2O2 biosensor roGFP2-Orp1 glutathione redox state GRX1-roGFP2 targeted various organelles reveal unsuspected events during pattern-triggered immune flagellin (flg22) after inoculation with...
Summary The reprogramming of gene expression appears as the major trend in synthetic and natural allopolyploids where an important proportion genes was shown to deviate from that parents or average parents. In this study, we analyzed changes previously reported, highly stable wheat allohexaploids combine D genome Aegilops tauschii AB extracted hexaploid Triticum aestivum . A comprehensive genome‐wide analysis transcriptional using Affymetrix GeneChip Wheat Genome Array conducted. Prevalence...
Plant stomata function in disease resistance by restricting bacteria entry inside leaves. During plant-bacteria interactions, stomatal closure is initiated the recognition of Microbe-Associated Molecular Patterns (MAMPs). Recently, we have shown that Lectin Receptor Kinase V.5 (LecRK-V.5) negatively regulates bacterium- and MAMP-induced upstream Reactive Oxygen Species (ROS) production mediated abscisic acid signaling. Closed lecrk-V.5 mutants are correlated with constitutive high level ROS...
Abstract Background Plant LIM domain proteins may act as transcriptional activators of lignin biosynthesis and/or actin binding and bundling proteins. genes have evolved in phylogenetic subgroups differing their expression profiles: the whole plant or specifically pollen. However, several poplar PtLIM belong to uncharacterized monophyletic patterns gene family a woody not been studied. Findings In this work, pattern twelve duplicated has investigated by semi quantitative RT-PCR different...
Abstract In the allopolyploid Brassica napus, we obtained a petal-closed flower mutation by ethyl methanesulfonate mutagenesis. Here, report cloning and characterization of Bn-CLG1A (CLG for cleistogamy) gene Bn-clg1A-1D mutant allele responsible cleistogamy phenotype. encodes RINGv E3 ubiquitin ligase that is highly conserved across eukaryotes. allele, C-to-T transition converts Pro at position 325 to Leu (P325L), causing dominant leading cleistogamy. B. napus Arabidopsis thaliana plants...
Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon 10) and stacei 20) that differ in chromosome evolution number. We created synthetic allotetraploids by hybridizing various lines of stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when was used as the maternal parent (0.15% or 0.245% depending on line used) sterile. No reciprocal crosses autotetraploids...
Polyploidization has played a prominent role in angiosperm evolution and speciation. Alterations of gene expression resulting from the merging two or more genomes are common features plants that may facilitate adaptative success. The bread allohexaploid wheat (Triticum aestivum, genome AABBDD) provides good example successful "man-made" allopolyploid evolved through relatively recent polyploidization events become most important crop cultivated worldwide. In present analysis, we review...
La panoplie des alliages cuivreux est extremement riche par la variete et combinaison additions employees et, aussi, leurs proportions souvent elevees (50 % au maximum toutefois). Avant guerre 1939-1945, moins 300 differents etaient couramment fabriques, sans compter bien d’autres compositions qui n’ont guere depasse un stade experimental. Depuis, on assiste a effort continu de rationalisation, technique economique, avec une polarisation vers les presentant proprietes rendant...
Abstract Dirigent (DIR) proteins mediate regio- and stereoselectivity of phenoxy radical coupling monolignols for (neo)lignan or lignin synthesis. However, the role DIR lignans in plant development response to environmental stresses remains elusive. Here, we provide a functional characterization cytokinin-responsive DIRIGENT13 ( DIR13 ) Arabidopsis thaliana . was localized root endodermis at margins lateral primordia roots. While not necessary Casparian strip formation, promoted both main...
Seedling de-etiolation is one of the key stages plant life cycle, characterized by a strong rearrangement development and metabolism. The conversion dark accumulated protochlorophyllide to chlorophyll in etioplasts de-etiolating plants taking place order ns µs after seedlings illumination, leading detectable increase levels minutes initiation. highly complex biosynthesis integrates number regulatory events including light hormonal signaling, thus making an ideal model study underlying...
ABSTRACT The enzymatic steps involved in l -ascorbate biosynthesis photosynthetic organisms (the Smirnoff-Wheeler, SW pathway) has been well established and here we comprehensively analyze the subcellular localization, potential physical interactions of pathway enzymes assess their role control ascorbate synthesis. Transient expression GFP-fusions Nicotiana benthamiana Arabidopsis ( thaliana ) mutants complemented with genomic constructs showed that while GME is cytosolic, VTC1, VTC2, VTC4,...
Abstract Despite an ever-increasing interest in reactive oxygen species (ROS) signalling during plant-microbe interactions, very little information exists, mainly for technical reasons, on the molecular mechanisms regulating intracellular hydrogen peroxide (H 2 O ) PAMP-triggered immunity. Here, we used a sensitive fluorimetry method and H sensor roGFP2-Orp1, which revealed unsuspected features regulation of cytoplasmic thiol redox dynamics upon pathogen-associated patterns (PAMPs)...