A5103168609- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Plant and animal studies
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Genetics and Plant Breeding
- Plant-Microbe Interactions and Immunity
- Sperm and Testicular Function
- Plant Ecology and Taxonomy Studies
- Plant Micronutrient Interactions and Effects
- Peanut Plant Research Studies
- Plant Surface Properties and Treatments
Huazhong Agricultural University
2016-2025
Wuhan College
2014-2023
Union Hospital
2005-2023
Huazhong University of Science and Technology
2005-2023
Shanghai Zhangjiang Laboratory
2023
Chinese Academy of Agricultural Sciences
2005-2018
Oil Crops Research Institute
2005-2018
Shaoguan University
2010
Oilseed rape (Brassica napus L.) was formed ~7500 years ago by hybridization between B. rapa and oleracea, followed chromosome doubling, a process known as allopolyploidy. Together with more ancient polyploidizations, this conferred an aggregate 72× genome multiplication since the origin of angiosperms high gene content. We examined consequences its recent duplication. The constituent An Cn subgenomes are engaged in subtle structural, functional, epigenetic cross-talk, abundant homeologous...
ABSTRACT Brassica napus , commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable worldwide. Identification and understanding gene functions in B. genome crucial for genomic breeding. A group genes controlling agronomic traits have been successfully cloned through functional genomics studies . In this review, we present an overview progress made including availability germplasm resources, omics databases genes. Based on current...
Flowering time adaptation is a major breeding goal in the allopolyploid species Brassica napus. To investigate genetic architecture of flowering time, genome-wide association study (GWAS) was conducted with diversity panel comprising 523 B. napus cultivars and inbred lines grown eight different environments. Genotyping performed 60K Illumina Infinium SNP array. A total 41 single-nucleotide polymorphisms (SNPs) distributed on 14 chromosomes were found to be associated 12 SNPs located...
S45A, a double recessive mutant at both the BnMs1 and BnMs2 loci in Brassica napus, produces no pollen mature anthers seeds by self-fertilization. The genes, which have redundant functions control of male fertility, are positioned on linkage groups N7 N16, respectively, located same locus Arabidopsis chromosome 1 based collinearity between Brassica. Complementation tests indicated that one candidate gene, BnCYP704B1, member cytochrome P450 family, can rescue sterility. Scanning electron...
Yellow seed is a desirable quality trait of the Brassica oilseed species. Previously, several coat color genes have been mapped in species, but molecular mechanism still unknown. In present investigation, map-based cloning method was used to identify gene, located on A9 B. rapa. Blast analysis with Arabidopsis genome showed that there were 22 this region including at4g09820 at4g10620. Functional complementation test exhibited phenotype reversion thaliana tt8-1 mutant and yellow-seeded plant....
Drought is a major threat to plant growth and crop productivity. Reduced level of the gibberellin would result in increased drought tolerance, but underlying mechanism still unclear. In Brassica napus, there are four BnaRGA genes that code for DELLA proteins, negative regulators GA signaling. Among them, expression BnaA6.RGA was greatly induced by abscisic acid (ABA). Previously, we created gain-of-function mutant BnaA6.RGA, bnaa6.rga-D, loss-of-function quadruple mutant, bnarga CRISPR/Cas9,...
Abstract The molecular mechanisms underlying anthocyanin-based flower coloration remain unknown in Brassica napus. To identify the key genes and metabolites associated with apricot pink colors, metabolome, BSA-seq, RNA-seq analyses were conducted on apricot-, pink-, yellow-, white-flowered F2B. Yellow carotenoids red anthocyanins abundant petals, while colorless accumulated petals. Most carotenoid not differentially regulated between yellow or white Three regulator genes, BnaMYBL2,...
Abstract Zeaxanthin epoxidase (ZEP) is a key enzyme that catalyzes the conversion of zeaxanthin to violaxanthin in carotenoid and abscisic acid (ABA) biosynthesis pathways. The rapeseed (Brassica napus) genome has 4 ZEP (BnaZEP) copies are suspected have undergone subfunctionalization, yet genes’ underlying regulatory mechanisms remain unknown. Here, we genetically confirmed functional divergence gene pairs BnaA09.ZEP/BnaC09.ZEP BnaA07.ZEP/BnaC07.ZEP, which encode enzymes with...
Cytoplasmic male sterility (CMS) is a widespread phenomenon in higher plants, and several studies have established that this maternally inherited defect often associated with mitochondrial mutant.Approximately 10 chimeric genes been identified as being corresponding CMS systems the family Brassicaceae, but there little direct evidence these cause sterility.In study, novel gene (named orf288) was found to be located downstream of atp6 co-transcribed hau sterile line.Western blotting analysis...
Harvest index (HI), the ratio of grain yield to total biomass, is considered as a measure biological success in partitioning assimilated photosynthate harvestable product. While crop production can be dramatically improved by increasing HI, underlying molecular genetic mechanism HI rapeseed remains shown.In this study, we examined architecture using 35,791 high-throughput single nucleotide polymorphisms (SNPs) genotyped Illumina BrassicaSNP60 Bead Chip an association panel with 155...
Here, we describe the characteristics of a Brassica napus male sterile mutant 7365A with loss BnMs3 gene, which exhibits abnormal enlargement tapetal cells during meiosis. Later in development, absence gene results secretory function tapetum, as suggested by abortive callose dissolution and retarded degradation. The BnaC.Tic40 (equivalent to BnMs3) was isolated map-based cloning approach confirmed genetic complementation. Sequence analyses that originated from BolC.Tic40 on oleracea linkage...
Soil salinity is a serious threat to agriculture sustainability worldwide. Salt tolerance at the seedling stage crucial for plant establishment and high yield in saline soils; however, little information available on rapeseed (Brassica napus L.) salt tolerance. We evaluated different accessions conducted genome-wide association study (GWAS) identify tolerance-related quantitative trait loci (QTL). A natural population comprising 368 B. cultivars inbred lines was genotyped with Brassica 60K...
Summary WRKY transcription factors are known to participate in the defence responses of higher plants. However, little is about roles such proteins, especially regarding their functions resistance oilseed rape ( Brassica napus ) Sclerotinia sclerotiorum , a necrotrophic fungal pathogen that causes stem rot. In this study, we identified Bn 33 as S. ‐responsive gene positively regulates by enhancing expression genes involved camalexin synthesis and regulated salicylic acid SA jasmonic JA ). We...
The Polima (pol) system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rfp have been used in hybrid breeding Brassica napus, which has greatly improved the yield rapeseed. However, mechanism transition pol CMS remains to be determined. To investigate transcriptome during CMS, a near-isogenic line (NIL) was constructed. phenotypic features stage were confirmed by anatomical analysis. Subsequently, we compared genomic expression profiles fertile sterile young flower...
Cytoplasmic male sterility (CMS) is not only important for exploiting heterosis in crop plants, but also as a model investigating nuclear-cytoplasmic interaction. CMS may be caused by mutations, rearrangement or recombination the mitochondrial genome. Understanding genome often first and key step unraveling molecular genetic basis of plants. Comparative analysis hau line its maintainer B. juneca (Brassica juncea) help show origin CMS-associated gene orf288. Through next-generation...
Brassica napus is currently cultivated as an important ornamental crop in China. Flower color has attracted much attention rapeseed genetics and breeding. Here, we characterize orange-flowered mutant of B. that exhibits altered carotenoid profile its petals. As revealed by map-based cloning, the change from yellow to orange attributed loss BnaC09.ZEP (zeaxanthin epoxidase) a 1695-bp deletion BnaA09.ZEP. HPLC analysis, genetic complementation CRISPR/Cas9 experiments demonstrated BnaA09.ZEP...
The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization, which drives genome evolution contributes to adaptability diverse environments. However, a comprehensive map Brassica napus still unavailable. In this study, we performed integrative analysis five histone modifications, RNA polymerase II occupancy, DNA methylation, transcriptomes in two B. lines (2063A B409), established global maps regulatory elements, chromatin...
Abstract Sclerotinia sclerotiorum causes substantial damage and loss of yield in oilseed rape (Brassica napus). The molecular mechanisms defense against remain elusive. In this study, we found that the early stages B. napus infection a conserved mitogen-activated protein kinase (MAPK) cascade mediated by BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module phosphorylates substrate BnWRKY33, enhancing its transcriptional activity. activated BnWRKY33 binds to own promoter triggers burst thus helping...
Doubled haploid technology is widely used to accelerate plant breeding, but its use in the important oilseed crop Brassica napus L. limited because B. haploids could only be obtained through vitro anther or microspore cultures. Recently, maize (Zea mays) lines containing mutations Domain of unknown function 679 membrane protein (DMP) were as inducer lines. This new induction mechanism has been extended several other plants, including dicots Arabidopsis thaliana, tomato (Solanum...
Plant architecture involves important agronomic traits affecting crop yield, resistance to lodging, and fitness for mechanical harvesting in Brassica napus. Breeding high-yield varieties with plant suitable is the main goal of rapeseed breeders. Here, we report an accession B. napus (4942C-5), which has a dwarf compact contrast cultivated varieties. A BC8 population was constructed by crossing normal line, 8008, recurrent parent 4942C-5. To investigate molecular mechanisms underlying...
Biosynthesis, stabilization, and storage of carotenoids are vital processes in plants that collectively contribute to the vibrant colors observed flowers fruits. Despite its importance, carotenoid pathway remains poorly understood lacks thorough characterization. We identified two homologous genes, BjA02.PC1 BjB04.PC2, belonging esterase/lipase/thioesterase (ELT) family acyltransferases. showed BjPCs association with fibrillin gene BjFBN1b control stable yellow Brassica juncea. Through...
Yellow seeds are a favorable trait for Brassica crops breeding due to better quality than their black-seeded counterparts. Here, we compared the napus seed coat transcriptomes between yellow- and brown-seeded near-isogenic lines (Y-NIL B-NIL) that were developed from resynthesized yellow-seeded line No. 2127-17. A total of 4,974 differentially expressed genes identified during development, involving 3,128 up-regulated 1,835 down-regulated in yellow coats. Phenylpropanoid flavonoid...
Drought stress is one of the major abiotic factors affecting Brassica napus (B. napus) productivity. In order to identify genes potential importance drought and obtain a deeper understanding molecular mechanisms regarding responses B. dehydration stress, we performed large-scale transcriptome sequencing plants under using Illumina technology. this work, relatively tolerant line, Q2, identified in our previous study, was used. Four cDNA libraries constructed from mRNAs control...
Plant height is a key morphological trait of rapeseed. In this study, we measured plant rapeseed population across six environments. This contains 476 inbred lines representing the major Chinese genepool and 44 from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype association panel. A genome-wide study (GWAS) performed via three methods, including robust, novel, nonparametric Anderson-Darling (A-D) test. Consequently, 68 loci were identified as significantly...