A5100762317- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Fungal and yeast genetics research
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Nitrogen and Sulfur Effects on Brassica
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Cassava research and cyanide
- Sulfur Compounds in Biology
- Pluripotent Stem Cells Research
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Pancreatic function and diabetes
- Genetic Mapping and Diversity in Plants and Animals
- Insect Resistance and Genetics
- Animal Genetics and Reproduction
- Redox biology and oxidative stress
- Nanoplatforms for cancer theranostics
- Transplantation: Methods and Outcomes
- IL-33, ST2, and ILC Pathways
Shanghai Ninth People's Hospital
2024
Shanghai Jiao Tong University
2014-2024
University of Illinois Urbana-Champaign
2023-2024
University of Electronic Science and Technology of China
2023-2024
Huazhong Agricultural University
2018-2023
Southwest University
2022
Peking University Cancer Hospital
2022
China Agricultural University
2022
Sweet Potato Research Institute
2022
Chinese Academy of Agricultural Sciences
2022
Abstract Epigenomic modifications are instrumental for transcriptional regulation, but comprehensive reference epigenomes remain unexplored in rice. Here, we develop an enhanced chromatin immunoprecipitation (eChIP) approach plants, and generate genome-wide profiling of five histone RNA polymerase II occupancy with it. By integrating accessibility, DNA methylation, transcriptome datasets, construct epigenome landscapes across various tissues 20 representative rice varieties. Approximately...
Abstract Insight into high-resolution three-dimensional genome organization and its effect on transcription remains largely elusive in plants. Here, using a long-read ChIA-PET approach, we map H3K4me3- RNA polymerase II (RNAPII)-associated promoter–promoter interactions H3K9me2-marked heterochromatin at nucleotide/gene resolution rice. The chromatin architecture is separated different independent spatial interacting modules with distinct transcriptional potential covers approximately 82% of...
Mucosal melanoma (MM) is the second most common subtype in Asian populations. Deregulation of microRNAs (miRNAs) has been extensively investigated various cancers, including cutaneous melanoma. However, roles miRNAs MM are unclear. In this study, we carried out miRNA profiling MM, and clinical biological miR-23a-3p MM. Methods: expression was profiled by microarray analysis. The quantitated qRT-PCR a cohort 117 patients with its prognostic significance evaluated. effect demonstrated both...
The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization, which drives genome evolution contributes to adaptability diverse environments. However, a comprehensive map Brassica napus still unavailable. In this study, we performed integrative analysis five histone modifications, RNA polymerase II occupancy, DNA methylation, transcriptomes in two B. lines (2063A B409), established global maps regulatory elements, chromatin...
Significance Notch is a critical regulator of skeletal development, but its role in remodeling the adult skeleton unclear. Using genetically modified mice, we show that stimulates mineralization by bone-building osteoblasts. Thus, overexpression intracellular domain mice results an increase bone mass, prevents loss following ovariectomy and during aging, promotes fracture healing. therefore potential therapeutic target for conditions loss, including osteoporosis.
Abstract Background The daily cycling of plant physiological processes is speculated to arise from the coordinated rhythms gene expression. However, dynamics diurnal 3D genome architecture and their potential functions underlying rhythmic expression remain unclear. Results Here, we reveal genome-wide occupancy RNA polymerase II (RNAPII), which precedes mRNA accumulation by approximately 2 h. Rhythmic RNAPII binding dynamically correlates with RNAPII-mediated chromatin remodeling at genomic...
Sulfonylurea (SU) herbicides inhibit branched-chain amino acid (BCAA) biosynthesis by targeting acetolactate synthase. Plants have evolved target-site resistance and metabolic tolerance to SU herbicides; the GCN2 (general control non-repressible 2) pathway is also involved in tolerance. Here, we report a novel mechanism, autophagy, which call 'homeostatic tolerance,' signaling Arabidopsis. The activation reversion of autophagy herbicide tribenuron-methyl (TM) exogenous BCAA, respectively,...
Plant C2-domain abscisic acid-related (CAR) protein family plays an important role in plant growth, abiotic stress responses, and defense regulation. In this study, we cloned the IbCAR1 by homologous cloning method from transcriptomic data of Xuzishu8, which is a sweet potato cultivar with dark-purple flesh. This gene was expressed all tissues potato, highest expression level leaf tissue, it could be induced NaCl ABA. Subcellular localization analyses indicated that localized nucleus plasma...
Des-gamma-carboxy prothrombin (DCP) is a serum protein produced by hepatocellular carcinoma (HCC) cells in the absence of vitamin K. Serum and tissue DCP expressions are thought to reflect biological malignant potential HCC. Hence, we aimed examine efficacy K(2) on production as well tumor cell growth invasion.Cell viability were evaluated 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The vivo was examined nude mice bearing HCC cells. A 24-well transwell chamber used...
<h3>Purpose</h3> To detect glutathione (GSH) in oral squamous carcinoma cells (OSCCs) with a GSH selective fluorescent probe during the course of oxidative stress and apoptosis. <h3>Materials Methods</h3> A novel was applied to assess human tongue cell (cal-27). The cellular reactive oxygen species (ROS) levels were assessed DCF-DA (2,7-dichlorofluorescin diacetate) probe. mitochondrial ROS probe, Mitotracker Red CM-H<sub>2</sub>XRos (Invitrogen, Carlsbad, CA). further study whether would...
Although utilization of heterosis has largely improved the yield many crops worldwide, underlying molecular mechanism heterosis, particularly for allopolyploids, remains unclear. Here, we compared epigenome and transcriptome data an elite hybrid its parental lines in three assessed tissues (seedling, flower bud, silique) to explore their contribution allopolyploid B. napus. Transcriptome analysis illustrated that a small proportion non-additive genes with parents, as well expression level...
While RNAs are soluble in vitro, their solubility may be altered when incorporated into some protein complexes inside the cell. The phase transition of is thus indicative changes function and activity RNAs. Here, we present a protocol for assessment RNA during Xenopus oocyte maturation. We describe steps sample preparation, cell fractionation, extraction, real-time PCR, analysis obtained results. For complete details on use execution this protocol, please refer to Hwang et al. (2023).1
In brief The impact of HVJ-E employed in mitochondrial replacement techniques (MRTs) on embryonic development remains uncertain. This study has exhibited the influence utilized MRTs and devised a novel HVJ-E-induced fusion approach to curtail amount MRTs. Abstract Mitochondrial provide viable option for women carrying pathogenic DNA (mtDNA) variants conceive disease-free offspring with genetic connection. comparison electrofusion, been identified as most promising clinical translation due...
The regulation of cellular metabolism and growth in response to nutrient availability is essential for cell survival can significantly impact lifespan. Central the a class transporters that sense transport specific nutrients transduce signal downstream control genes responsible survival. In this work, we identified SUL1, plasma membrane transporter regulating entry extracellular sulfate S. cerevisiae , as key gene We performed systematic analysis delineate mechanism underlying lifespan...
The regulation of cellular metabolism and growth in response to nutrient availability is essential for cell survival can significantly impact lifespan. Central the a class transporters that sense transport specific nutrients transduce signal downstream control genes responsible survival. In this work, we identified SUL1, plasma membrane transporter regulating entry extracellular sulfate S. cerevisiae , as key gene We performed systematic analysis delineate mechanism underlying lifespan...
Abstract Primordial germ cells (PGCs) are the precursors of sperms and oocytes. Proper development PGCs is crucial for survival species. In many organisms, factors responsible PGC synthesized during early oogenesis assembled into plasm. During embryonic development, plasm inherited by a few cells, leading to formation PGCs. While germline has been extensively studied, how components regulate not fully understood. Here, we report that Dzip1 dynamically expressed in vertebrate novel component...