A5100683451- Plant Disease Resistance and Genetics
- Lipid metabolism and biosynthesis
- Nitrogen and Sulfur Effects on Brassica
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Antioxidant Activity and Oxidative Stress
- Plant Pathogens and Resistance
- Soybean genetics and cultivation
- Horticultural and Viticultural Research
- Plant Genetic and Mutation Studies
- Nematode management and characterization studies
- Studies on Chitinases and Chitosanases
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Berry genetics and cultivation research
- GABA and Rice Research
- CRISPR and Genetic Engineering
Huazhong Agricultural University
2016-2025
Shanghai Zhangjiang Laboratory
2024-2025
Southwest University
2024
Zhongshan Hospital
2024
Fudan University
2024
Fujian Agriculture and Forestry University
2022-2024
Qingdao University of Science and Technology
2021-2024
University of Benin
2024
Jilin Academy of Agricultural Sciences
2017-2024
Heilongjiang Bayi Agricultural University
2019-2024
ABSTRACT Brassica napus , commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable worldwide. Identification and understanding gene functions in B. genome crucial for genomic breeding. A group genes controlling agronomic traits have been successfully cloned through functional genomics studies . In this review, we present an overview progress made including availability germplasm resources, omics databases genes. Based on current...
Yellow seed coat color (SCC) is a valuable trait in Brassica napus , which significantly correlated to high oil content (SOC) and low lignocellulose (SLC). However, no dominant yellow SCC genes were identified B. . In this study, N53-2 was verified, then 58,981 eQTLs 25 trans-eQTL hotspots double haploid population derived from black material Ken-C8. A rare allele DYSOC1 ( gene of improved 1 ) subsequently cloned hotspot that colocated with SCC, SOC, SLC QTL on ChrA09 through fine mapping...
• Two ethylmethanesulfonate (EMS) mutant populations of the semi-winter rapeseed cv. Ningyou7 were constructed with high load, to provide a TILLING platform for functional genomics in Brassica napus, and introduction novel allelic variation breeding. Forward genetic screening mutants from M2 resulted identification large number phenotypes. Reverse focused on potentially multi-paralogous gene FAE1 (fatty acid elongase1), which controls seed erucic synthesis rapeseed. A B. napus BAC library...
Although Plasmodiophora brassicae is one of the most common pathogens worldwide, causal agent clubroot disease in Brassica crops, resistance mechanisms to it are still only poorly understood. To study early defense response induced by P. infection, a global transcriptome profiling roots two near-isogenic lines (NILs) clubroot-resistant (CR BJN3-2) and clubroot-susceptible (BJN3-2) Chinese cabbage (Brassica rapa) was performed RNA-seq. Among 42,730 unique genes mapped reference genome B....
Sclerotinia stem rot (SSR) caused by the necrotrophic fungus sclerotiorum is a major disease in rapeseed (Brassica napus) worldwide. Breeding for SSR resistance B. napus, as other crops, relies only on germplasms with quantitative genes. A better understanding of genetic basis napus thus holds promise improvement resistance. In present study, genome-wide association study (GWAS) were performed using an panel 448 accessions genotyped Brassica 60K Infinium® single-nucleotide polymorphism (SNP)...
Abstract Crossovers create genetic diversity and are required for equal chromosome segregation during meiosis. Crossover number distribution highly regulated by different mechanisms that not yet fully understood, including crossover interference. The axis is crucial formation. Here, we explore the function of protein ASYNAPSIS3. To this end, use allotetraploid species Brassica napus; due to its polyploid nature, system allows a fine-grained dissection dosage meiotic regulators. simultaneous...
Summary Grain size is a crucial agronomic trait that affects stable yield, appearance, milling quality, and domestication in rice. However, the molecular genetic relationships among QTL genes (QTGs) underlying natural variation for grain remain elusive. Here, we identified novel QTG SGW5 ( suppressor of gw5 ) by map‐based cloning using an F 2 segregation population fixing same genotype master GW5 . positively regulates width influencing cell division spikelet hulls. Two nearly isogenic lines...
The biosynthesis of the tocotrienol and tocopherol forms vitamin E is initiated by prenylation homogentisate. Geranylgeranyl diphosphate (GGDP) prenyl donor for synthesis, whereas phytyl (PDP) synthesis. We have previously shown that synthesis in monocot seeds homogentisate geranylgeranyl transferase (HGGT). This enzyme related to phytyltransferase (HPT), which catalyzes step Here we show HGGT localized plastid expressed primarily seed endosperm. Despite close structural relationship HPT,...
Plasmodiophora brassicae, the causal agent of clubroot disease Brassica crops, is widespread in world. Quantitative trait loci (QTLs) for partial resistance to 4 different isolates P. brassicae (Pb2, Pb4, Pb7, and Pb10) were investigated using a BC1F1 population from cross between two subspecies rapa, i.e. Chinese cabbage inbred line C59-1 as susceptible recurrent parent turnip ECD04 resistant donor parent. The BC1F2 families assessed under controlled conditions. A linkage map constructed...
A comprehensive linkage atlas for seed yield in rapeseed. Most agronomic traits of interest crop improvement (including yield) are highly complex quantitative controlled by numerous genetic loci, which brings challenges comprehensively capturing associated markers/genes. We propose that multiple trait interactions underlie such as yield, and considering these component their can dissect individual loci (QTL) effects more effectively improve predictions. Using a segregating rapeseed (Brassica...
Abstract Phytyl diphosphate (PDP) is the prenyl precursor for tocopherol biosynthesis. Based on recent genetic evidence, PDP supplied to biosynthetic pathway primarily by chlorophyll degradation and sequential phytol phosphorylation. Three enzymes of Arabidopsis (Arabidopsis thaliana) are known be capable removing chain from in vitro: chlorophyllase1 (CLH1), CLH2, pheophytin pheophorbide hydrolase (PPH), which specifically hydrolyzes pheophytin. While PPH, but not chlorophyllases, required...
High-density single nucleotide polymorphisms (SNPs) are used as highly favored makers to analyze genetic diversity and population structure, construct high-density maps provide genotypes for genome-wide association analysis. In order develop SNP markers in oil palm (Elaeis guineensis), SLAF-seq (Single Locus Amplified Fragment sequencing) technology was performed a panel of 200 individuals 1 261 501 SNPs were identified with minor allele frequency > 0.05 integrity 1. Among them, only 17.81%...
Summary Glucosinolates (GSLs), whose degradation products have been shown to be increasingly important for human health and plant defence, compose secondary metabolites found in the order Brassicales. It is highly desired enhance pest disease resistance by increasing leaf GSL content while keeping low seeds of Brassica napus , one most oil crops worldwide. Little known about regulation accumulation leaves. We quantified levels 9 different GSLs 15 related traits leaves 366 accessions that...
Summary Vitamin E tocotrienol synthesis in monocots requires homogentisate geranylgeranyl transferase ( HGGT ), which catalyzes the condensation of and unsaturated C20 isoprenoid diphosphate GGDP ). By contrast, vitamin tocopherol is mediated by phytyltransferase HPT condenses saturated phytyl PDP An ‐independent pathway for has also been shown to occur de‐regulation synthesis. In this paper, basis its impact on production when combined with are explored. Arabidopsis line was initially...
Chlorophyll synthase catalyzes the final step in chlorophyll biosynthesis: esterification of chlorophyllide with either geranylgeranyl diphosphate or phytyl (PDP). Recent studies have pointed to involvement chlorophyll-linked reduction by reductase as a major pathway for synthesis PDP precursor tocopherols. This indirect suggests key role tocopherol production generate geranylgeranyl-chlorophyll substrate reductase. In this study, contributions formation Arabidopsis (Arabidopsis thaliana)...
Very long chain fatty acids (VLCFAs) with lengths of 20 carbons and longer provide feedstocks for various applications; therefore, improvement VLCFA contents in seeds has become an important goal oilseed enhancement. biosynthesis is controlled by a multi-enzyme protein complex referred to as acid elongase, which composed β-ketoacyl-CoA synthase (KCS), reductase (KCR), β-hydroxyacyl-CoA dehydratase (HCD) enoyl (ECR). KCS been identified the rate-limiting enzyme, but little known about...
Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO activity vivo an experimental model with potassium oxonate-induced hyperuricemia mice which used evaluate anti-hyperuricemic activity. crude showed expressive urate-lowering results. Solvent partitioning followed by macroporous resin column chromatography n-butanol yielded four...
Rapeseed (B. napus, AACC, 2n = 38) is one of the most important oil seed crops in world, it also common for production biodiesel. Its a mixture various fatty acids and dissection genetic network biosynthesis great importance improving quality.The basis acid B. napus was investigated via quantitative trail locus (QTL) analysis using doubled haploid (DH) population with 202 lines. A total 72 individual QTLs large number pairs epistatic interactions associated content 10 different were...