A5015709922- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Research in Cotton Cultivation
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Cancer, Lipids, and Metabolism
- Plant Pathogens and Resistance
- Genomics, phytochemicals, and oxidative stress
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Phytochemistry and Biological Activities
- Oil Palm Production and Sustainability
- Plant Stress Responses and Tolerance
- Powdery Mildew Fungal Diseases
- Plant responses to water stress
- Advances in Cucurbitaceae Research
- Bioinformatics and Genomic Networks
Huazhong University of Science and Technology
2015-2024
Ministry of Education of the People's Republic of China
2023
Huanggang Normal University
2018-2020
Center of Hubei Cooperative Innovation for Emissions Trading System
2015
Abstract Late embryogenesis abundant (LEA) proteins are a diverse and large group of polypeptides that play important roles in desiccation freezing tolerance plants. The LEA family has been systematically characterized some plants but not Brassica napus . In this study, 108 BnLEA genes were identified the B. genome classified into eight families based on their conserved domains. Protein sequence alignments revealed an abundance alanine, lysine glutamic acid residues proteins. gene structure...
Summary Drought is an abiotic stress that affects plant growth, and lipids are the main economic factor in agricultural production of oil crops. However, molecular mechanisms drought response function lipid metabolism remain little known. In this study, overexpression ( OE ) different copies genes LEA 3 VOC enhanced both tolerance content Brassica napus Arabidopsis . Meanwhile, seed size, membrane stability weight were also improved lines. contrast, decreased At mutant atlea3 ‐ RNA i Bn B....
Increasing seed oil content is one of the most important goals in breeding rapeseed (B. napus L.). To dissect genetic basis B. napus, a large and new double haploid (DH) population containing 348 lines was obtained from cross between 'KenC-8' 'N53-2', two varieties with >10% difference content, this named KN DH population. A linkage map consisting 403 markers constructed, which covered total length 1783.9 cM an average marker interval 4.4 cM. The phenotyped eight natural environments...
Brassica napus is one of the most important oilseed crops, and can supply considerable amounts edible oil as well provide raw materials for production biodiesel in biotechnology industry. Lysophosphatidic acid acyltransferase (LPAT), a key enzyme Kennedy pathway, catalyses fatty chains into 3-phosphoglycerate promotes further form triacylglycerol. However, because B. an allotetraploid with two subgenomes, precise genes which involved remain unclear due to intractability efficiently knocking...
Rapeseed (B. napus, AACC, 2n = 38) is one of the most important oil seed crops in world, it also common for production biodiesel. Its a mixture various fatty acids and dissection genetic network biosynthesis great importance improving quality.The basis acid B. napus was investigated via quantitative trail locus (QTL) analysis using doubled haploid (DH) population with 202 lines. A total 72 individual QTLs large number pairs epistatic interactions associated content 10 different were...
Rapeseed is the third largest oil seed crop in world. The seeds of this plant store lipids bodies, and oleosin most important structural protein bodies. However, function crops has received little attention.In present study, 48 sequences from Brassica napus genome were identified divided into four lineages (T, U, SH, SL). Synteny analysis revealed that genes conserved, all these experienced purifying selection during evolution. Three Arabidopsis B. napus, respectively, cloned analyzed for...
The recently constructed mutant libraries of diploid crops by the CRISPR-Cas9 system have provided abundant resources for functional genomics and crop breeding. However, because genome complexity, it is a big challenge to accomplish large-scale targeted mutagenesis in polyploid plants. Here, we demonstrate feasibility using pooled CRISPR library achieve genome-scale editing an allotetraploid Brassica napus . A total 18,414 sgRNAs were designed target 10,480 genes interest, afterward, 1104...
Abstract Brassica rapa and Raphanus sativus are two important edible vegetables that contain numerous nutritional ingredients. However, the agronomic traits components of intergeneric hybrid B . R remain poorly understood. In this study, we used a stably inherited as model to study its metabolome transcriptome profiles. Morphological cytological analysis showed had expected chromosome number normal meiosis behavior. Moreover, multiple secondary metabolites, including flavonoids...
Ultrastructural observations, combined with proteomic and comparative genomic analyses, were applied to interpret the differences in protein composition oil-body characteristics of mature seed two Brassica napus lines high low oil contents 55.19% 36.49%, respectively. The results showed that bodies arranged much closer than content line, cell size thickness walls also observed. There 119 32 differentially expressed proteins (DEPs) total identified. DEPs mainly involved oil-related,...
Trichoderma harzianum TH12 is a microbial pesticide for certain rapeseed diseases. The mechanism of systemic resistance induced by or its cell-free culture filtrate (CF) in Brassica napus (AACC) and Raphanus alboglabra (RRCC) to powdery mildew disease caused ascomycete Erysiphe cruciferarum was investigated. In this study, we conducted the first large-scale global study on cellular molecular aspects B. R. infected with E. cruciferarum. histological showed disease. growth fungal colonies not...
Raphanus sativus is an important Brassicaceae plant and also edible vegetable with great economic value. However, currently there not enough transcriptome information of R. tissues, which impedes further functional genomics research on sativus. In this study, RNA-seq technology was employed to characterize the leaf tissues. Approximately 70 million clean pair-end reads were obtained used for de novo assembly by Trinity program, generated 68,086 unigenes average length 576 bp. All annotated...
Raphanus sativus is not only a popular edible vegetable but also an important source of medicinal compounds. However, the paucity knowledge about transcriptome R. greatly impedes better understanding functional genomics and potential sativus. In this study, sequencing leaf tissues in was performed for first time. Approximately 22 million clean reads were generated used assembly. The unigenes subsequently annotated against gene ontology (GO) database. KEGG analysis further revealed two...
Brassica napus is an important oilseed crop that offers a considerable amount of biomass for global vegetable oil production. The establishment efficient genetic transformation system with convenient transgenic-positive screening method great importance gene functional analysis and molecular breeding. However, to our knowledge, there are few the aforementioned systems available application in B. napus.Based on well-established napus, five vectors carrying red fluorescence protein encoding...
Vicinal oxygen chelate proteins (VOC) are members of the metalloenzyme superfamily, which plays roles in many biological reactions. Some VOC superfamily have been systematically characterized but not Brassica napus. In this study, 38 genes were identified based on their conserved domains. The present results revealed that most BnaVOC few introns, and all contained typical structure βαβββ modules. distributed unevenly across 15 chromosomes B. napus occur as gene clusters C5 A6. synteny...
Abstract Background Increasing seed oil content (SOC) of Brassica napus has become one the main plant breeding goals over past decades. Lysophosphatidic acid acyltransferase (LPAT) performs an important molecular function by regulating production phosphatidic (PA), a key intermediate in synthesis membrane and storage lipids. However, mechanism underlying effect LPAT on SOC B. remains unclear. Results In present study, significant elevation was achieved overexpressing BnLPAT2 BnLPAT5 . RNAi...
Acyl-coA binding proteins (ACBPs) bind long chain acyl-CoA esters with very high affinity. Their possible involvement in fatty acid transportation from the plastid to endoplasmic reticulum, prior formation of triacylglycerol has been suggested. Four classes ACBPs were identified Arabidopsis thaliana: small ACBPs, large ankyrin repeats containing and kelch motif ACBPs. They differed structure size, showed multiple important functions. In present study, Brassica napus characterized. Eight...
Brassica napus is the most important oilseed crop in world, and lipid was stored oil body (OB) form of triacylglycerol. At present, studies on relationship between morphology seed content B. focused mature seeds. In present study, OBs different developing seeds with relatively high (HOC) about 50% low (LOC) 39% were analyzed. It revealed that size first increased then decreased both materials. And late developmental stages, average OB rapeseed HOC higher than LOC, while it reversed early...
Fatty acid desaturases add a second bond into single of carbon atoms in fatty chains, resulting an unsaturated between the two carbons. They are classified soluble and membrane-bound desaturases, according to their structure, subcellular location, function. The orthologous genes Camelina sativa were identified analyzed, total 62 desaturase identified. It was revealed that they had common domain, which has evolved separately, proteins same family also originated from ancestry. A mix...
Rapeseed is the second most important oil crop in world. Improving seed yield and content are two main highlights of research. Unfortunately, rapeseed development frequently affected by different diseases. Extensive research has been made through many years to develop elite cultivars with high oil, yield, and/or disease resistance. Quantitative trait locus (QTL) analysis one strategies genetic deciphering agronomic characteristics. To comprehend distribution these QTLs uncover key regions...
For the past decade, research on constructing large-scale plant protein–protein interaction (PPI) networks has mainly focused model plants, and capability of experimental-based PPI in these plants was thought to be significantly inferior actual number protein interactions (Altmann et al., 2020; Jones 2014; Smakowska-Luzan 2018; Wang 2023). Currently, there is still a lack experimentally-derived, data especially with respect specific seed organs (Rhee Mutwil, 2014). In this study, we selected...
Abstract Background Increasing seed oil content is the most important breeding goal in Brassica napus , and phenotyping crucial to dissect its genetic basis crops. To date, QTL mapping for has been based on whole seeds, lipid distribution far from uniform different tissues of seeds B. . In this case, phenotype was unable sufficiently reveal complex characteristics content. Results Here, three-dimensional (3D) determined by magnetic resonance imaging (MRI) 3D quantitative analysis, ten novel...