A5090908099- Nitrogen and Sulfur Effects on Brassica
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Cell death mechanisms and regulation
- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Nuclear Structure and Function
- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Food Quality and Safety Studies
- Plant pathogens and resistance mechanisms
- Genomics, phytochemicals, and oxidative stress
- Peanut Plant Research Studies
- Heat shock proteins research
- Genetic Mapping and Diversity in Plants and Animals
- Soybean genetics and cultivation
- Phosphodiesterase function and regulation
- Environmental and Agricultural Sciences
- Phytase and its Applications
- Plant Physiology and Cultivation Studies
- Spectroscopy and Chemometric Analyses
- Plant Pathogens and Resistance
- Advanced Frequency and Time Standards
- Rice Cultivation and Yield Improvement
Xinjiang Medical University
2023
Hybrid Rapeseed Research Center of Shaanxi Province
2010-2022
National Taiwan Normal University
2015-2022
Zhejiang University of Technology
2022
National Institute of Biological Sciences, Beijing
2015-2021
Tsinghua University
2019-2021
Beijing Normal University
2015-2016
Northwest A&F University
2013
ZheJiang Academy of Agricultural Sciences
2013
Tianma Microelectronics (China)
2012
Receptor-interacting protein kinase 3, RIP3, and a pseudokinase mixed lineage kinase-domain like protein, MLKL, constitute the core components of necroptosis pathway, which causes programmed necrotic death in mammalian cells. Latent RIP3 cytosol is activated by several upstream signals including related RIP1, transduces from tumor necrosis factor (TNF) family cytokines. We report here that activation following induction requires activity an HSP90 CDC37 cochaperone complex. This complex...
A pair of kinases, RIPK1 and RIPK3, as well the RIPK3 substrate MLKL cause a form programmed necrotic cell death in mammals termed necroptosis. We report here that male reproductive organs both Ripk3- Mlkl-knockout mice retain ‘youthful’ morphology function into advanced age, while those age-matched wild-type deteriorate. The phosphorylation MLKL, activation marker necroptosis, is detected spermatogonial stem cells testes old but not young mice. When are given local necroptotic stimulus,...
Increasing seed oil content is one of the most important goals in breeding rapeseed (B. napus L.). To dissect genetic basis B. napus, a large and new double haploid (DH) population containing 348 lines was obtained from cross between 'KenC-8' 'N53-2', two varieties with >10% difference content, this named KN DH population. A linkage map consisting 403 markers constructed, which covered total length 1783.9 cM an average marker interval 4.4 cM. The phenotyped eight natural environments...
Background: Ulcerative colitis (UC) and irritable bowel syndrome (IBS) share various similarities in clinical symptoms, pathogenesis, treatment. UC concurrent IBS tends toward more severe symptoms worse prognosis, promising feasible therapies for the overlapping remains a challenge. Rhubarb peony decoction (RPD) is well-known traditional Chinese medicine that has been widely applied treating UC. RPD may exert extensive therapeutic effects on both However, common mechanism of its treatment...
Realistic and accurate simulations are crucial for developing quantum sensors such as cold atom gyroscopes, gravimeters, gradiometers in the field of interferometers (CAIs). However, current lack necessary specifications CAIs. In this study, we constructed a physical model CAIs designed detailed simulation program, including construction, input output parameter selection, program design, computational acceleration. The energy levels were rationally simplified approximated based on optical...
Abstract Molecular glues are a class of small molecular drugs that mediate protein-protein interactions, induce either the degradation or stabilization target protein. A structurally diverse group chemicals, including 17-β-estradiol (E2), anagrelide, nauclefine, and DNMDP, induces apoptosis by forming complexes with phosphodiesterase 3A (PDE3A) Schlafen 12 protein (SLFN12). They do so binding to PDE3A enzymatic pocket allows compound-bound recruit stabilize SLFN12, which in turn blocks...
Abstract Prostaglandin F 2α (PGF ), an endogenous arachidonic acid metabolite, regulates diverse physiological functions in many tissues and cell types through binding activation of a G-protein-coupled receptor (GPCR), the PGF (FP), which also is primary therapeutic target for glaucoma several other diseases. Here, we report cryo-electron microscopy (cryo-EM) structures human FP bound to ligand anti-glaucoma drugs LTPA TFPA at global resolutions 2.67 Å, 2.78 3.14 Å. These reveal distinct...
Casein kinases are a large family of intracellular serine/threonine that control variety cellular signaling functions. Here we report member casein kinase 1 family, 1G2, CSNK1G2, binds and inhibits the activation receptor-interacting 3, RIPK3, thereby attenuating RIPK3-mediated necroptosis. The binding CSNK1G2 to RIPK3 is triggered by auto-phosphorylation at serine 211/threonine 215 sites in its C-terminal domain. CSNK1G2-knockout mice showed significantly enhanced necroptosis response...
Ultrastructural observations, combined with proteomic and comparative genomic analyses, were applied to interpret the differences in protein composition oil-body characteristics of mature seed two Brassica napus lines high low oil contents 55.19% 36.49%, respectively. The results showed that bodies arranged much closer than content line, cell size thickness walls also observed. There 119 32 differentially expressed proteins (DEPs) total identified. DEPs mainly involved oil-related,...
Abstract The promotion of apoptosis in tumor cells is a popular strategy for developing anti-cancer drugs. Here, we demonstrate that the plant indole alkaloid natural product nauclefine induces diverse cancer via PDE3A-SLFN12 dependent death pathway. Nauclefine binds PDE3A but does not inhibit PDE3A’s phosphodiesterase activity, thus representing previously unknown type modulator can initiate without affecting canonical function. We H840, Q975, Q1001, and F1004 residues—as well as I105...
Oil bodies (OBs) are relatively simple but very important organelles comprising a matrix of triacylglycerol (TAG) surrounded by phospholipid monolayer embedded and covered with unique proteins. The OB structure in Brassica napus different oil content the relationship between needs to be better understood. In this paper, characteristics OBs embryo series B. materials ranging from 34% over 60% were studied. results indicated that size was significantly positively correlated negatively...
Cytoplasmic male sterility (CMS) lines are widely used for hybrid production in Brassica napus. The Shaan2A CMS system is one of the most important China and has been decades; however, mechanism underlying remains unknown. Here, we performed transcriptomic proteomic analysis, combined with additional morphological observation, CMS. Sporogenous cells, endothecium, middle layer, tapetum could not be clearly distinguished anthers. Furthermore, anther chloroplasts contained fewer starch grains...
Receptor-interacting serine/threonine-protein kinase 3 (RIPK3) normally signals to necroptosis by phosphorylating MLKL. We report here that when the cellular RIPK3 chaperone Hsp90/CDC37 level is low, also apoptosis. The apoptotic function of requires phosphorylation serine 165/threonine 166 sites on its activation loop, resulting in inactivation activity while gaining ability recruit RIPK1, FADD, and caspase-8 form a cytosolic caspase-activating complex, thereby triggering found PGF 2α...
The Arabidopsis homeotic genes APETALA3 (AP3) and PISTILLATA (PI) are B which encode MADS-box transcription factors specify petal stamen identities. In the current study, carpelloid (SC) mutants, HGMS AMS, of B. rapa napus were investigated two types AP3 genes, B.AP3.a B.AP3.b, functional characterized. B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at C-terminus. Loss this led to change PI-derived motifs. Meanwhile, specified development,...
Abstract Background Cytoplasmic male sterility (CMS) is very important in hybrid breeding. The restorer-of-fertility ( Rf ) nuclear genes rescue the sterile phenotype. Most of encode pentatricopeptide repeat (PPR) proteins. Results We investigated restorer-of-fertility-like RFL gene family Brassica napus . A total 53 BnRFL were identified. While most distributed on 10 19 chromosomes, clusters identified chromosomes A9 and C8. number PPR motifs proteins varied from 2 to 19, majority harbored...