A5063016502- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Nitrogen and Sulfur Effects on Brassica
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant Molecular Biology Research
- RNA modifications and cancer
- Plant Ecology and Taxonomy Studies
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Nuts composition and effects
- Plant and Fungal Interactions Research
- Genetics and Plant Breeding
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant pathogens and resistance mechanisms
- Fetal and Pediatric Neurological Disorders
- Plant Stress Responses and Tolerance
- Neurogenetic and Muscular Disorders Research
- MicroRNA in disease regulation
- Plant Surface Properties and Treatments
- Genetic diversity and population structure
- Genetic and phenotypic traits in livestock
University of Chicago
2021-2024
Huazhong Agricultural University
2014-2023
Wuhan College
2022
Shandong University of Science and Technology
2019
Flowering time adaptation is a major breeding goal in the allopolyploid species Brassica napus. To investigate genetic architecture of flowering time, genome-wide association study (GWAS) was conducted with diversity panel comprising 523 B. napus cultivars and inbred lines grown eight different environments. Genotyping performed 60K Illumina Infinium SNP array. A total 41 single-nucleotide polymorphisms (SNPs) distributed on 14 chromosomes were found to be associated 12 SNPs located...
Harvest index (HI), the ratio of grain yield to total biomass, is considered as a measure biological success in partitioning assimilated photosynthate harvestable product. While crop production can be dramatically improved by increasing HI, underlying molecular genetic mechanism HI rapeseed remains shown.In this study, we examined architecture using 35,791 high-throughput single nucleotide polymorphisms (SNPs) genotyped Illumina BrassicaSNP60 Bead Chip an association panel with 155...
Abstract Sclerotinia sclerotiorum causes substantial damage and loss of yield in oilseed rape (Brassica napus). The molecular mechanisms defense against remain elusive. In this study, we found that the early stages B. napus infection a conserved mitogen-activated protein kinase (MAPK) cascade mediated by BnaA03.MKK5-BnaA06.MPK3/BnaC03.MPK3 module phosphorylates substrate BnWRKY33, enhancing its transcriptional activity. activated BnWRKY33 binds to own promoter triggers burst thus helping...
Abstract The nuclear N 6 ‐methyladenosine (m A) reader YT521‐B homology‐domain‐containing protein 1 (YTHDC1) is required to maintain embryonic stem cell identity. However, little known about its biological functions in intestinal‐resident macrophages and inflammatory bowel disease (IBD). Herein, it demonstrated that macrophage‐specific depletion or insufficiency of YTHDC1 accelerates IBD development animal models. On the molecular basis, reduction IBD‐derived attributed Zinc finger 36...
Yellow seeds are a favorable trait for Brassica crops breeding due to better quality than their black-seeded counterparts. Here, we compared the napus seed coat transcriptomes between yellow- and brown-seeded near-isogenic lines (Y-NIL B-NIL) that were developed from resynthesized yellow-seeded line No. 2127-17. A total of 4,974 differentially expressed genes identified during development, involving 3,128 up-regulated 1,835 down-regulated in yellow coats. Phenylpropanoid flavonoid...
Drought stress is one of the major abiotic factors affecting Brassica napus (B. napus) productivity. In order to identify genes potential importance drought and obtain a deeper understanding molecular mechanisms regarding responses B. dehydration stress, we performed large-scale transcriptome sequencing plants under using Illumina technology. this work, relatively tolerant line, Q2, identified in our previous study, was used. Four cDNA libraries constructed from mRNAs control...
Plant height is a key morphological trait of rapeseed. In this study, we measured plant rapeseed population across six environments. This contains 476 inbred lines representing the major Chinese genepool and 44 from other countries. The 60K Brassica Infinium® SNP array was utilized to genotype association panel. A genome-wide study (GWAS) performed via three methods, including robust, novel, nonparametric Anderson-Darling (A-D) test. Consequently, 68 loci were identified as significantly...
Abstract The rapeseed branch angle is an important morphological trait because adequate enables more efficient light capture under high planting densities. Here, we report that the average of five top branches provides a reliable representation all branches. Statistical analyses revealed significantly positive correlation between and multiple plant-type yield-related traits. 60 K Brassica Infinium ® single nucleotide polymorphism (SNP) array was utilized to genotype association panel with...
Helitron is a rolling-circle DNA transposon; it plays an important role in plant evolution. However, distribution and contribution to evolution at the family level have not been previously investigated.We developed software easy-to-annotate (EAHelitron), Unix-like command line, used identify Helitrons wide range of 53 genomes (including 13 Brassicaceae species). We determined density (abundance/Mb) visualized examined patterns. identified more than 104,653 Helitrons, including many new...
Chimeric genes contribute to the evolution of diverse functions in plants and animals. However, new chimeric also increase risk developmental defects. Here, we show that gene Brassica napus male sterile 4 (Bnams4b) is responsible for genic sterility widely used canola line 7365A (Bnams3 ms3ms4bms4b). Bnams4b originated via exon shuffling ∼4.6 million years ago. It causes defects normal plastids induces aborted anther formation and/or albino leaves buds. Evidence age mutation, its tissue...
Alloplasmic lines, in which the nuclear genome is combined with wild cytoplasm, are often characterized by cytoplasmic male sterility (CMS), regardless of whether it was derived from sexual or somatic hybridization relatives. In this study, we sequenced and analyzed mitochondrial genomes five such alloplasmic lines Brassica juncea.The assembled annotated were found to have virtually identical gene contents. They preserved most ancestral segments, same candidate (orf108) harbored...
Abstract As a desirable agricultural trait, multilocular trait of rapeseed ( Brassica rapa ; napus juncea ), always represents higher yield per plant compared with bilocular plants. We previously isolated trilocular gene locus, Bjmc1 , and identified set molecular markers linked to the gene. With map-based cloning, we that BjMc1 was located in B genome it CLAVATA1 CLV1 ) homologue. The insertion copia-LTR retrotransposable element 1 (RTE1) into coding region interrupted its transcription...
Timely flowering is important for seed formation and maximization of rapeseed (Brassica napus) yield. Here, we performed flowering-time quantitative trait loci (QTL) mapping using a double haploid (DH) population grown in three environments to study the genetic architecture. Brassica 60 K Illumina Infinium™ single nucleotide polymorphism (SNP) array simple sequence repeat (SSR) markers were used genotyping DH population, high-density linkage map was constructed. QTL analysis time from...
Differential polyadenylation sites (PAs) critically regulate gene expression, but their cell type-specific usage and spatial distribution in the brain have not been systematically characterized. Here, we present Infernape, which infers quantifies PA from single-cell transcriptomic data show its application mouse brain. Infernape uncovers alternative intronic PAs 3'-UTR lengthening during cortical neurogenesis. Progenitor-neuron comparisons excitatory inhibitory neuron lineages overlapping...
Abstract Combining ability is crucial for parent selection in crop hybrid breeding. The present investigation and results had revealed the underlying genetic factors which might contribute adequate combining ability, further assisting enhancing heterosis stability. Here, we conducted a large-scale analysis of genomic variation order to define regions affecting recurrent population rapeseed. A 175 individuals was genotyped with Brassica60K SNP chip. 525 hybrids were assembled three different...
Abstract Gene duplication followed by functional divergence in the event of polyploidization is a major contributor to evolutionary novelties. The Brassica genus evolved from common ancestor after whole-genome triplication. Here, we studied and features spp. homologs Tic40 (for translocon at inner membrane chloroplasts with 40 kDa). Four loci were identified allotetraploid napus two each three basic diploid Although these share high sequence identities similar expression patterns, they...
Abstract Hypocotyl elongation is considered an important typical seedling trait contributing directly to increase in and stabilization of the yield Brassica napus , but its molecular genetic mechanism poorly understood. In present study, hypocotyl lengths 210 lines were measured illuminated culture room. A genome-wide association study (GWAS) was performed with 23,435 single nucleotide polymorphisms (SNPs) for length. Three long length three short from one doubled haploid line (DH)...
Heterosis is an important biological phenomenon in which the hybrids exceed parents many traits. However, molecular mechanism underlying seedling heterosis remains unclear.
The cuticle is a layer that covers the surfaces of land plants composed cutin and cuticular wax, which forms hydrophobic barrier against environmental damage. At5g02890 encodes novel protein with unknown function in Arabidopsis thaliana. In current study, sequence analysis showed highly conserved Brassicaceae. lines overexpressing (OE-At5g02890 lines) an orthologous gene from Brassica napus (OE-Bn1 exhibited glossy stems. Chemical revealed overexpression caused significant reductions levels...
Despite heterosis contributing to genetic improvements in crops, root growth rapeseed plants is poorly understood at the molecular level. The current study was performed discover key differentially expressed genes (DEGs) related two hybrids with contrasting performance (FO; high hybrid and FV; low hybrid) based on analysis of effect. Based comparative transcriptomic analysis, we believe that overdominance gene expression level plays a critical role roots’ early biomass heterosis. Our...
Abstract RNA-binding proteins (RBPs) are essential for gene regulation, but it remains a challenge to identify their RNA targets across cell types. Here we present PIE-Seq investigate Protein-RNA Interaction with dual-deaminase Editing and Sequencing by conjugating C-to-U A-to-I base editors RBPs. We benchmark demonstrate its sensitivity in single cells, application the developing brain, scalability 25 human Bulk identifies canonical binding features RBPs such as PUM2 NOVA1, nominates...