- Plant pathogens and resistance mechanisms
- Genetic and Environmental Crop Studies
- Seed and Plant Biochemistry
- Genetics and Plant Breeding
- Advances in Cucurbitaceae Research
- Nitrogen and Sulfur Effects on Brassica
- Agricultural pest management studies
- Genetically Modified Organisms Research
- Phytase and its Applications
- Plant Pathogenic Bacteria Studies
- Botanical Research and Chemistry
- Microbial Metabolites in Food Biotechnology
- Chromosomal and Genetic Variations
- Food composition and properties
- Agronomic Practices and Intercropping Systems
- Genetic diversity and population structure
- Potato Plant Research
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Plant Micronutrient Interactions and Effects
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Physiology and Cultivation Studies
- Garlic and Onion Studies
Agricultural institute of Slovenia
2016-2025
Pulses are edible seeds of plants belonging to the legume family, which great importance for human and animal nutrition. In this study, several nutrients, antinutrients bioactive compounds were quantified in ten pulses, i.e., common runner beans, field peas, lupins (white, blue yellow), faba lentils (brown red) chickpeas. Homogenised, air-dried seed samples analysed various parameters: protein (18.0–43.1%), fat (0.6–18.5%) phytic acid content (507–2566 mg/100 g dry weight (DW)), phenolic...
Introduction The color of the seed coat common bean ( Phaseolus vulgaris L.) is an important trait influencing marketability and consumer preferences. An understanding genetic mechanisms underlying variation can aid in breeding programs aimed at improving esthetic agronomic traits. This study investigates diversity molecular associated with change composite populations through phenotypic analysis whole genome sequencing (WGS). Methods Four two standard varieties were cultivated over a...
Grain legumes are important for sustainable agriculture due to their agronomic and nutritional properties. In this study, a rapid, high-throughput, non-destructive seed screening was performed on 835 lines of Phaseolus vulgaris , Lupinus albus L. mutabilis grown under sub-alpine climate. The were analysed size characteristics (thousand weight – TSW, width length) using MARVIN analyser protein moisture content near-infrared transmittance spectroscopy. results showed strong correlation between...
Genetic resources comprised of 953 accessions common (Phaseolus vulgaris L.) and 47 runner coccineus bean from the national Slovene gene bank were characterized using fourteen morphological seed descriptors. Seeds each accession evaluated for six quantitative characteristics: length, thickness, width, length/width ratio, width/thickness 100 or 10 weight. Furthermore, seeds eight qualitative colour; number colours; primary/main predominant secondary distribution veining; shape; colour...
Barley, oats, or spelt consumed as minimally processed whole grains provide several health benefits, especially when grown under organic field management conditions. Therefore, the effects of and conventional farming on compositional traits (protein, fibre, fat, ash) barley, oat, groats were compared using three winter barley varieties ('Anemone', 'BC Favorit', 'Sandra'), two spring oat ('Max' 'Noni'), ('Ebners Rotkorn', 'Murska bela', 'Ostro'). Groats produced from harvested by a...
Common buckwheat (Fagopyrum esculentum) is an ancient crop with a world-wide distribution. Due to its excellent nutritional quality and high economic ecological value, common becoming increasingly important throughout the world. The availability of high-quality reference genome sequence population genomic data will accelerate breeding buckwheat, but heterozygosity due outcrossing nature has greatly hindered assembly. Here we report assembly chromosome-scale F. esculentum var. homotropicum,...
Agriculture faces great challenges to overcome global warming and improve system sustainability, requiring access novel genetic diversity. So far, wild populations local landraces remain poorly explored. This is notably the case for two diploid species, Brassica oleracea L. (CC, 2n=2x=18) B. rapa (AA, 2n=2x=20). In order explore diversity in both we have collected their centre of origin, Mediterranean basin, on a large contrasting climatic soil gradient from northern Europe southern...
In this study, genetic diversity of 119 accessions common bean ( Phaseolus vulgaris ) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set markers has proven before to efficiently distinguish between genotypes and assign them either Andean or Mesoamerican gene pool origin. 118 alleles were detected 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, Serbian) showed similarly high levels as estimated...
Abstract Background Common bean ( Phaseolus vulgaris L.) is the most important food legume for direct human consumption around world, as it represents a valuable source of components with nutritional and health benefits. Results We conducted study to define explain genetic relatedness diversification level common germplasm from Portugal Ukraine, along western-to-eastern line southern European countries, including Poland. This was based on P. structure, designed better describe its...
Understanding the genetic background of drought tolerance in common bean (Phaseolus vulgaris L.) can aid its resilience improvement. However, response studies large seeded genotypes Andean origin are insufficient. Here, a novel intra-gene pool linkage map was created for quantitative trait locus (QTL) mapping drought-responsive traits recombinant inbred line population from cross two cultivars differing their to drought. Single environment and QTL × analysis revealed 49 QTLs physiology,...
Characterisation of genetic diversity is critical to adequately exploit the potential germplasm collections and identify important traits for breeding programs sustainable crop improvement. Here, we characterised phenotypic a global collection two cultivated buckwheat species Fagopyrum esculentum tataricum (190 51 accessions, respectively) using 37 agro-morphological 24 SSR markers. A wide range variation was observed in both most analysed. The differed significantly traits, with related...
Analysis of gene flow between Brassica napus L. and its sexually compatible relatives that could be found in the wild Slovenia was performed by microsatellite analysis using fifteen selected primer pairs. Genotypes included study were obtained from field survey B. natural habitats around reference collections. Two different species all presented Slovenia, rapa Sinapis arvensis. The genotypes varieties forms internal collections as marketable seeds or banks. Reference represented following...
Extraction of high quality DNA is crucial for any molecular genetic analysis. However, it difficult to be obtained from problematic plant tissue, in phenolic compounds, such as apple leaves. Despite the variety commercially available kits isolation, no study has been done so far evaluating their potential tree. We have tested six different and compared performance on five ten samples tree (Malus X domestica) Genomic was extracted following manufacturers' protocols amplified by touchdown PCR...
French beans are tender, immature, edible pods that harvested early in the plant's growth cycle and usually eaten cooked. The habits of were studied for first time a Citizen Science experiment, 19 pod samples collected further nutritional analysis. Various macronutrients (e.g., protein, ash, fat, carbohydrates, amino acids) multi-element profiles determined. A survey their growing revealed planted once or twice year May June at length 5-10 m, with predominance dwarf cultivation over climbing...
Different methods for the extraction of trypsin inhibitors in beans (Phaseolus spp.) were investigated. Two randomised complete laboratory experiments performed, one on seeds and pods. In first, common bean variety KIS Marcelijan, breeding line Ref_316 × 498 runner Bonela examined. second, fresh pods five (three lines, two varieties) analysed. Four used, including ultrasonic-assisted (UAE) 15 30 min shaking-assisted 60 180 min. The results showed a significant increase inhibitor...
Buckwheat is a highly nutritional pseudocereal with antioxidant potential. The aim of this study was to analyze the genetic variability 21 varieties common buckwheat (Fagopyrum esculentum Moench.) and 14 Tartary tataricum Gaertn.) using microsatellite markers. By analyzing SSR markers, an average 11.6 alleles per locus were amplified PIC value 0.711 determined. We determined heterozygous status individuals in set analysis on basis expected heterozygosity (He, 0.477), observed (Ho, 0.675),...
Lupinus albus is a food grain legume recognized for its high levels of seed protein (30[ndash]40%) and oil (6[ndash]13%), adaptability to different climatic soil conditions. To develop the next generation L. cultivars, we need access well-characterized, genetically phenotypically diverse germplasm. Here evaluated more than 2000 accessions with passport data based on 35 agro-morphological traits Intelligent CORE Collections. The reference (R-CORE), representing global diversity, exemplified...
The genetic composition of sweet potato [Ipomoea batatas (L.) Lam.] (2n = 6x 90) is reflected in different levels variation. main objective the present study was a comparison diversity parameters ploidy (2n, 4n, and 6n codominant allele determination), analyzed on two high-resolution capillary platforms. Fragment analysis performed by applying SSR markers 3130XL Genetic Analyzer (ABI 3130) QIAxcel Advanced System (QX). A high level expected heterozygosity (He) as measure observed for both...
In South-Eastern Europe, the majority of runner-bean (Phaseolus coccineus L.) production is based on local populations grown mainly in home gardens. The plants are well adapted to their specific growing conditions and microclimate agro-environments, show great morpho-agronomic diversity. Here, 142 accessions from five European countries Slovenia, Bosnia Herzegovina, Serbia, North Macedonia Romania were sown cultivated respective characterised using 28 quantitative qualitative descriptors for...
Abstract Kale ( Brassica oleracea. var. acephala ) is a nutrient-rich green leafy vegetable consumed as food and used in traditional medicine worldwide. An essential step describing the available genetic resources ensuring their effective use breeding programs to characterize diversity of germplasm. In this study, structure 26 kale accessions from South-East Europe were examined using agro-morphological traits collected field 12 simple sequence repeat (SSR) markers. Considerable variability...
Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, adaptability to extreme conditions, disease resistance, low cultivation inputs, make it particularly suitable by resource-poor farmers. However, the potential use of grass limited due presence anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed develop...