A5066813304- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Protein Structure and Dynamics
- MicroRNA in disease regulation
- Chemical Synthesis and Analysis
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Coronary Interventions and Diagnostics
- Genomics and Chromatin Dynamics
- Electrolyte and hormonal disorders
- Ion channel regulation and function
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Genomics and Phylogenetic Studies
- Erythrocyte Function and Pathophysiology
- Antiplatelet Therapy and Cardiovascular Diseases
- RNA modifications and cancer
- Atrial Fibrillation Management and Outcomes
- Cancer-related gene regulation
- Machine Learning in Bioinformatics
- Vitamin D Research Studies
- Tuberculosis Research and Epidemiology
Fondation Jean Dausset-CEPH
2019-2025
Second Affiliated Hospital of Zhejiang University
2018-2024
Zhejiang University
2021-2024
Zhejiang Lab
2024
Tongji University
2023
Lanzhou University
2023
Lanzhou University Second Hospital
2023
Nanjing Medical University
2022
Centre National de Recherche en Génomique Humaine
2018-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2021
Peptides and mini proteins have many biological biomedical implications, which motivates the development of accurate methods, suitable for large-scale experiments, to predict their experimental or native conformations solely from sequences. In this study, we report PEP-FOLD2, an improved coarse grained approach peptide de novo structure prediction compare it with PEP-FOLD1 state-of-the-art Rosetta program. Using a benchmark 56 structurally diverse peptides 25–52 amino acids total 600...
In the context of renewed interest peptides as therapeutics, it is important to have an on-line resource for 3D structure prediction with well-defined structures in aqueous solution. We present updated version PEP-FOLD allowing treatment both linear and disulphide bonded cyclic 9-36 amino acids. The server makes possible define bonds any residue-residue proximity under guidance biologists. Using a benchmark 34 one, two three bonds, best models deviate by average RMS 2.75 Å from full NMR...
Abstract Despite intense investigation, human replication origins and termini remain elusive. Existing data have shown strong discrepancies. Here we sequenced highly purified Okazaki fragments from two cell types and, for the first time, quantitated fork directionality delineated initiation termination zones genome-wide. Replication initiates stochastically, primarily within non-transcribed, broad (up to 150 kb) that often abut transcribed genes, terminates dispersively between them....
Hemodynamic overload induces pathological cardiac hypertrophy, which is an independent risk factor for intractable heart failure in long run. Beyond neurohumoral regulation, mechanotransduction has been recently recognized as a major regulator of hypertrophy under myriad conditions. However, the identification and molecular features mechanotransducer on cardiomyocytes are largely sparse. For first time, we identified Piezo1 (Piezo type mechanosensitive ion channel component 1), novel with...
Ventricular arrhythmogenesis is a key cause of sudden cardiac death following myocardial infarction (MI). Accumulating data show that ischemia, sympathetic activation, and inflammation contribute to arrhythmogenesis. However, the role mechanisms abnormal mechanical stress in ventricular arrhythmia MI remain undefined. We aimed examine impact increased identify sensor Piezo1 MI. Concomitant with pressure, Piezo1, as newly recognized mechano-sensitive cation channel, was most up-regulated...
Microsatellites are short tandem repeats (STRs) of a motif 1-6 nucleotides that ubiquitous in almost all genomes and widely used many biomedical applications. However, despite the development next-generation sequencing (NGS) over past two decades with new technologies coming to market, accurately genotyping STRs, particularly homopolymers, remain very challenging today due several technical limitations. This leads cases erroneous allele calls difficulty correctly identifying genuine...
Metastatic melanoma can be treated with anti-PD-1 monotherapy or in combination anti-CTLA-4 anti-Lag3. However, therapy is associated a high risk of toxicity. Recently, we reported that plasma soluble CD27 (sCD27) levels reflect the intratumoral interaction CD70-CD27 and dysfunctional T cells tumor microenvironment renal cell carcinoma. In this study, first characterized expression CD70 tumors their vivo. We then significant association between baseline sCD27 resistance as assessed by...
Significance Taking advantage of two markers critically associated with ATP metabolism, CD39 ectonucleotidase and the adenosine deaminase binding factor CD26, we found that natural regulatory T cells (nTregs) are heterogenous, composed five major structurally genetically distinct cell subsets, each representing a stage nTreg maturation. Three outcomes foreseeable from these studies: ( i ) immunologically, microenvironmental factors dictate developmental evolution govern their functional...
Quiescent caner stem cells are identified as a subpopulation of colon cancer in dormant state and possess strong stem-cell like characteristics. Previously, we have this colorectal (quiescent cells, QCCSCs), find QCCSCs sensitive to the apoptotic effect IFN-γ, which is attributed their high IFN-γR expression levels. Microarray bioinformatic analysis indicate miR-4666-3p low expressed target IFN-γR1/2, proved by luciferase assay western-blot. Furthermore, could also TGF-βR1 block activation...
Abstract Background In this study, we aimed to identify bone mineral density (BMD) trajectories of hospitalised patients with coronavirus disease 2019 (COVID-19) and determine the prognostic role trajectory groups. Methods This is a retrospective study COVID-19 treated in our hospital from November 2022 February 2023. BMD was manually measured thoracic 12 (T12) lumbar one (L1) vertebra using chest computed tomography images. We constructed group models group-based modelling. performed...
Predicting accurate fragments from sequence has recently become a critical step for protein structure modeling, as fragment assembly techniques are presently among the most efficient approaches de novo prediction. A key in these is, given of to model, identification relevant - candidate collection available 3D structures. These can then be assembled produce model complete interest. The search is classically achieved by considering local similarity using profile comparison, or threading...
Abstract Background Insulin autoimmune syndrome (IAS) is a rare cause of hypoglycemia and characterized by the presence insulin autoantibodies. Patients with IAS usually complain without any previous received. Glucocorticoids immunosuppressants are used to treat IAS. Case presentation We report four patients diabetes who were diagnosed non-classical describe treatment these patients. Moreover, differential diagnosis hyperinsulinism discussed. Conclusion High levels autoantibodies, as well...
Aim: Nonhuman primates are essential for research on many human diseases. The Infinium Human Methylation450/EPIC BeadChips popular tools the study of methylation state across genome at affordable cost. Methods: We performed a precise evaluation and re-annotation BeadChip probes analysis genome-wide DNA patterns in rhesus macaques African green monkeys through silico analyses combined with functional validation by pyrosequencing. Results: Up to 165,847 450K 261,545 EPIC can be reliably used....
Growing evidence suggests that epigenetic mechanisms like microRNA-mediated transcriptional regulation contribute to the pathogenesis of parkinsonism. In order study influence microRNAs (miRNAs), we analyzed miRNome 2 days prior major cell death in α-synuclein-overexpressing Lund human mesencephalic neurons, a well-established model Parkinson’s disease (PD), by next-generation sequencing. The expression levels 23 miRNAs were significantly altered cells, 11 down- and 12 upregulated ( P...
Abstract Although unequivocal evidence has shown the prognostic relevance of circulating tumor cells (CTCs) in patients with metastatic breast cancer (MBC), less is available for its significance neoadjuvant chemotherapy (NCT) early (BC). Here we conducted an analysis individual data from 86 confirmed as invasive BC by core‐needle biopsy Zhejiang Provincial People's Hospital between June 2013 and January 2017. The CTCs were assessed at time after diagnosis before surgery CanPatrol technique....