A5087623059- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cardiovascular Disease and Adiposity
- Adipose Tissue and Metabolism
- Chemokine receptors and signaling
- Adipokines, Inflammation, and Metabolic Diseases
- SARS-CoV-2 detection and testing
- Phagocytosis and Immune Regulation
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- RNA modifications and cancer
- Reproductive System and Pregnancy
- interferon and immune responses
- RNA regulation and disease
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Pneumonia and Respiratory Infections
- Thermoregulation and physiological responses
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Digestive system and related health
- HIV Research and Treatment
Université de Strasbourg
2020-2024
Hôpital Civil, Strasbourg
2021-2024
Inserm
2020-2024
Centre National de la Recherche Scientifique
2024
Instituts Universitaires de Technologie
2024
Karlsruhe Institute of Technology
2022
Université d'Avignon et des Pays de Vaucluse
2015-2020
Immuno-Rhumathologie moléculaire
2019
Université Claude Bernard Lyon 1
2013
ABSTRACT The roles of the extracellular matrix molecule tenascin-C (TNC) in health and disease have been extensively reviewed since its discovery over 40 years ago. Here, we will describe recent insights into TNC tumorigenesis, angiogenesis, immunity metastasis. In addition to high levels expression tumors, during chronic inflammation, bacterial viral infection, is also expressed lymphoid organs. This supports potential for control. Advances using murine models with engineered were...
Metastasis is a major cause of death in cancer patients. The extracellular matrix molecule tenascin-C known promoter metastasis, however the underlying mechanisms are not well understood. To further analyze impact on progression we generated MMTV-NeuNT mice that develop spontaneous mammary tumors, knockout background. We also developed syngeneic orthotopic model which tumor cells derived from tumor. Tumor were transfected with control shRNA or to knockdown expression and, grafted into gland...
Abstract Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet roles tumor immunity are incompletely understood. Using 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, expression...
The interplay between cancer cells and immune is a key determinant of tumor survival. Here, we uncovered how tumors exploit the immunomodulatory properties extracellular matrix to create microenvironment that enables their escape from surveillance. Using orthotopic grafting mammary in immunocompetent mice autochthonous models breast cancer, discovered tenascin-C, molecule absent most healthy adult tissues but expressed at high levels associated with poor patient prognosis many solid cancers,...
Tracks rich in matrix and cells, as described several cancer types, have immunosuppressive functions separate tumor nests stroma, yet their origin is unknown. Immunostainings of cryosections from mouse breast tumors show that these tracks are bordered by an endothelial-like basement membrane, filled with fibers collagen adjacent to tenascin-C (TNC) low-tension fibronectin (Fn) fibers. While present early-stage maturing time, still form under TNC KO conditions, however, host (not...
Abstract Enhancement of Wnt signaling is fundamental for stem cell function during intestinal regeneration. Molecular modules control activity by regulating signal transduction. CD44 such a positive regulator and target gene. While highly expressed in crypts used as marker, its role homeostasis regeneration remains unknown. Here we propose positive-feedback loop that boosts transduction, thus impacting Excision Cd44 fl/fl ;VillinCreER T2 mice reduced gene expression affected functionality...
The extracellular matrix (ECM) molecule Tenascin-C (TNC) is well-known to promote tumor progression by multiple mechanisms. However, reliable TNC detection in tissues of banks remains limited. Therefore, we generated dromedary single-domain nanobodies Nb3 and Nb4 highly specific for human (hTNC) characterized the interaction with several approaches including ELISA, western blot, isothermal fluorescence titration negative electron microscopic imaging. Our results revealed binding both...
Tumor-targeted therapies have often been inefficient due to the lack of concomitant control over tumor microenvironment. Using an immunocompetent autologous breast cancer model, we investigated a MAtrix REgulating MOtif (MAREMO)-mimicking peptide, which inhibits protumorigenic extracellular matrix (ECM) molecule tenascin-C that activates several hallmarks. In cultured cells, targeting MAREMO blocks signaling involved in cell adhesion and immune-suppression by inhibiting interactions with...
Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) are severe inflammatory disorders that often involve focal necrotizing glomerulonephritis (FNGN) and consequent glomerular scarring, interstitial fibrosis, chronic kidney disease. Robust murine models of scarring in FNGN may help to further our understanding deleterious processes still lacking. Here, we present a model based on combined administration antibodies against the basement membrane (GBM) myeloperoxidase...
Bidirectional dialogue between cellular and non-cellular components of the tumor microenvironment (TME) drives cancer survival. In extracellular space, combinations matrix molecules soluble mediators provide external cues that dictate behavior TME resident cells. Often studied in isolation, integrated from complex tissue microenvironments likely function more cohesively. Here, we study interplay molecule tenascin-C (TNC) chemokine CCL2, both elevated associated with progression breast...
Wastewater-based epidemiology is experiencing exponential development. Despite undeniable advantages compared to patient-centered approaches (cost, anonymity, survey of large populations without bias, detection asymptomatic infected peoples…), major technical limitations persist. Among them the low sensitivity current methods used for quantifying and sequencing viral genomes from wastewater. In situations circulation, during initial stages emergences, or in areas heavy rains, extremely...
Radiotherapy, the most frequent treatment of oral squamous cell carcinomas (OSCC) besides surgery is employed to kill tumor cells but, radiotherapy may also promote relapse where immune-suppressive microenvironment (TME) could be instrumental. We established a novel syngeneic grafting model from carcinogen-induced tongue tumor, OSCC13, address impact on OSCC. This revealed similarities with human OSCC, recapitulating mutations found in smoking associated tumors, abundant infiltrating...
BCL11b is a transcription regulator and tumor suppressor involved in lymphomagenesis, central nervous system (CNS) immune developments. favors persistence of HIV latency contributes to control cell cycle, differentiation apoptosis multiple organisms models. Although recruits the non-coding RNA 7SK epigenetic enzymes regulate gene expression, BCL11b-associated ribonucleoprotein complexes are unknown. Thanks CLIP-seq quantitative LC-MS/MS mass spectrometry approaches complemented with systems...
Age-related diseases are major concern in developed countries. To avoid disabilities that accompany increased lifespan, pharmaceutical approaches considered. Therefore, appropriate animal models required for a better understanding of aging processes and potential vivo assays to evaluate the impact molecules may delay occurrence age-related diseases. Few mouse exhibiting pathological exist, but currently, none them reproducibly mimics human like osteoporosis, cognitive dysfunctions or...
Adipose tissue (AT) homeostasis and growth are dependent on microvasculature. This capillary network has a large remodeling capacity, process called angio-adaptation. In response to metabolic alterations linked obesity, signaling pathways involved in the maintenance of vascular AT appear be affected. this context, we studied C57/Bl6 subject an high fat diet (HFD) voluntary exercise protocol 7weeks, capillarisation epididymal (eWAT) subcutaneous adipose (sWAT) by histological marking CD31,...
<div>Abstract<p>The interplay between cancer cells and immune is a key determinant of tumor survival. Here, we uncovered how tumors exploit the immunomodulatory properties extracellular matrix to create microenvironment that enables their escape from surveillance. Using orthotopic grafting mammary in immunocompetent mice autochthonous models breast cancer, discovered tenascin-C, molecule absent most healthy adult tissues but expressed at high levels associated with poor patient...