A5073088630- Venomous Animal Envenomation and Studies
- Monoclonal and Polyclonal Antibodies Research
- Toxin Mechanisms and Immunotoxins
- Ion channel regulation and function
- Biosimilars and Bioanalytical Methods
- Cell Adhesion Molecules Research
- Biochemical and Structural Characterization
- Research on Leishmaniasis Studies
- Escherichia coli research studies
- Iron Metabolism and Disorders
- Antibiotic Resistance in Bacteria
- Neuroscience and Neural Engineering
- Antimicrobial Peptides and Activities
- Animal health and immunology
- Erythrocyte Function and Pathophysiology
- Viral Infections and Vectors
- Vibrio bacteria research studies
- Cardiac electrophysiology and arrhythmias
- Erythropoietin and Anemia Treatment
- Glycosylation and Glycoproteins Research
- Yersinia bacterium, plague, ectoparasites research
- Papaya Research and Applications
- Blood properties and coagulation
- Venous Thromboembolism Diagnosis and Management
- Phytoplasmas and Hemiptera pathogens
Institut Pasteur de Tunis
1995-2022
Tunis El Manar University
2016-2022
Tunis University
2016-2022
Institut Pasteur
1985
Envenoming following scorpion sting is a common emergency in many parts of the world. Our aim was to ameliorate current 100-kDa horse plasma antivenom serum (PAS)-derived Fab'(2) more quickly reach highly diffusible toxins (7 kDa). We immunized dromedaries with from Androctonus australis hector (Aah) scorpions and cloned single-domain antibody fragments or nanobodies (15 kDa) their B cells. Nanobodies against AahI' toxin (with AahII most toxic compound venom) were retrieved libraries,...
Summary: We have cloned and expressed the gp63 gene of Leishmania major in BCG to develop a recombinant vaccine against zoonotic cutaneous leishmaniasis. Two different expression systems were investigated. The first system consists pAN, Mycobacterium paratuberculosis promoter, which drives ORF2, an open reading frame IS900. This allows production heterologous polypeptides as hybrids with ORF2 product. second relies on antigenic fragments fusion proteins N-terminal region fortuitum...
Scorpion venom, containing highly toxic, small polypeptides that diffuse rapidly within the patient, causes serious medical problems. Nanobodies, single-domain antigen-binding fragments derived from dromedary heavy-chain antibodies, have a size closely matches of scorpion toxins. Therefore these nanobodies might be developed into potent immunotherapeutics to treat envenoming. Multiple sub-nanomolar affinity AahII, most toxic polypeptide Androctonus australis hector were isolated immunized...
The extracellular matrix (ECM) molecule Tenascin-C (TNC) is well-known to promote tumor progression by multiple mechanisms. However, reliable TNC detection in tissues of banks remains limited. Therefore, we generated dromedary single-domain nanobodies Nb3 and Nb4 highly specific for human (hTNC) characterized the interaction with several approaches including ELISA, western blot, isothermal fluorescence titration negative electron microscopic imaging. Our results revealed binding both...
Scorpion envenoming is a severe health problem in many regions causing significant clinical toxic effects and fatalities. In the Middle East/North Africa (MENA) region, Buthidae scorpion stings are responsible for devastating outcomes human. The only available specific immunotherapeutic treatment based on IgG fragments of animal origin. To overcome limitations classical immunotherapy, we have demonstrated vivo efficacy NbF12-10 bispecific nanobody at preclinical level. Nanobodies were...
Leishmaniasis is the 3rd most challenging vector-borne disease after malaria and lymphatic filariasis. Currently, no vaccine candidate approved or marketed against leishmaniasis due to difficulties in eliciting broad immune responses when using sub-unit vaccines. The aim of this work was design a particulate for vaccination leishmaniasis. poly (D,L-lactide) nanoparticles (PLA-NPs) were developed order efficiently adsorb recombinant L. major histone H2B (L. H2B) boost its immunogenicity....
New Delhi Metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase, able to hydrolyze almost all antibiotics β-lactam group, leading multidrug-resistant bacteria. To date, there are no clinically relevant inhibitors fight NDM-1. The use dromedary polyclonal antibody against NDM-1 represents a promising new class molecules with inhibitory activity. In current study, immunoreactivities Immunoglobulin G (IgG) isotypes containing heavy-chain and conventional antibodies...
The potency of a recombinant human erythropoietin (rhEPO) pharmaceutical preparation was evaluated by the normocythaemic mice bioassay using standardized and randomized protocol European Pharmacopeia 5.0. Eight-week-old B6D2F1/c received single daily injections test solutions (EPOTIN erythropoietin, batch preparation) at different concentrations. Erythropoietin biological reference product (batch 3.3) used as standard. suitability recorded according to gender age. blood sampling performed 4...