A5101503234- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Healthcare and Venom Research
- Biochemical and Structural Characterization
- Blood Coagulation and Thrombosis Mechanisms
- Insect Resistance and Genetics
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Bacillus and Francisella bacterial research
- Phytochemicals and Antioxidant Activities
- Erythrocyte Function and Pathophysiology
- Insect Pest Control Strategies
- Natural product bioactivities and synthesis
- Antimicrobial Peptides and Activities
- Nicotinic Acetylcholine Receptors Study
- Cell death mechanisms and regulation
- Cytomegalovirus and herpesvirus research
- Study of Mite Species
- Respiratory viral infections research
- Salmonella and Campylobacter epidemiology
- Bioactive Natural Diterpenoids Research
- Bone Metabolism and Diseases
Institut Pasteur de Tunis
2004-2019
Tunis University
2016-2018
Tunis El Manar University
2013-2018
Institut Pasteur
1996-1997
Resistance to 5-Fluorouracil chemotherapy is a major cause of therapeutic failure in colon cancer cure. Development combined therapies constitutes an effective strategy inhibit cells and prevent the emergence drug resistance. For this purpose, we investigated anti-tumoral effect thirteen phenolic compounds, from Tunisian quince Cydonia oblonga Miller, alone or 5-FU, on human 5-FU-resistant LS174-R comparison parental cells. Our results showed that only Kaempferol was able chemo-sensitize...
Lebein, is an heterodimeric disintegrin isolated from Macrovipera lebetina snake venom that was previously characterized as inhibitor of ADP-induced platelet aggregation. In this study, we investigated the effect Lebein on p53-dependent growth human colon adenocarcinoma cell lines. We found significantly inhibited LS174 (p53wt), HCT116 and HT29 (p53mut) cancer viability by inducing cycle arrest through modulation expression levels tumor suppression factor p53, regulating proteins cyclin D1,...
Development of alternative cancer-specific drugs would be paramount importance to overcome toxicity toward normal tissues and tumor resistance. Here, we investigated the potential anti-tumoral effect peel (Peph) pulp polyphenolic extracts from Tunisian quince Cydonia oblonga Miller on both no-tumorigenic cells NIH 3T3 Fibroblasts HEK 293 human colon adenocarcinoma LS174 cells.Cell proliferation cytotoxicity were measured with MTT LDH assays respectively. Cell cycle distribution apoptosis...
We report the chemical composition and anti- Leishmania antioxidant activity of Artemisia campestris L. herba - alba Asso. essential oils (EOs). Our results showed that these extracts exhibit different activities according to used assay. The radical scavenging effects determined by DPPH assay were IC 50 = 3.3 mg/mL 9.1 for oils, respectively. However, both ferric-reducing power (FRAP) assay, in same range (2.3 2.97 mg eq EDTA/g EO, resp.), while oil highest chelating Fe 2+ ions (27.48 mM )....
Polyphenols, flavonoids and condensed tannins contents, as well the antioxidant activity of ethanolic aqueous extracts obtained from aerial parts 10 wild Tunisian plants, have been determined. Extracts showed appreciable levels polyphenols flavonoids, which reached 215.16 mg GAE g-1 DW in Lavandula stoechas extract, 49.12 RE Thapsia garganica extract. The majority tested exhibited low total content, except for Rhus tripartitum Periploca laevigata. tests great activity, especially R....
Melanoma, the most threatening form of skin cancer, has a very poor prognosis and is characterized by its invasive chemoresistant properties. Despite recent promising news from field immunotherapy, there an urgent need for new therapeutic approaches that are free resistance mechanisms side effects. Anti-neoplasic properties have been highlighted different disintegrins snake venom including Lebein; however, exact effect Lebein on melanoma not yet defined. In this study, we showed blocks cell...
We have constructed a cDNA library from venom glands of the scorpion Buthus occitanus tunetanus and cloned DNA sequence that encodes an α‐toxin. This clone was efficiently expressed in Escherichia coli as fusion protein with two Ig‐binding (Z) domains A Staphylococcus aureus. After CNBr treatment HPLC purification, we obtained approximately 1 mg recombinant α‐toxin/I bacterial culture. The toxin, called Bot XIV, displays no toxicity towards mammals but is active insects shown by its...
Coronary endothelial dysfunction is involved in cardiac ischemia-reperfusion (IR) injury. Vascular growth factor (VEGF) activates cells and exerts cardioprotective effects isolated hearts. The recently discovered viper venom protein called increasing capillary permeability (ICPP) VEGF-like cells. We examined whether VEGF or ICPP can influence IR outcome vivo mice. Dosages of were determined by preliminary blood pressure study. In IR, both the proteins administered intravenously at...
Cerastotin, a thrombin‐like enzyme from the venom of desert viper Cerastes cerastes , has been purified by gel filtration on Sephadex G‐75 and two ion‐exchange chromatographies Mono S columns. It is neutral glycoprotein (pI = 6.6), present as single polypeptide chain 40 kDa. Its N‐terminal sequence shows strong similarity with those other enzymes snake venoms. Cerastotin possesses esterase amidolytic activities measured N α ‐tosyl‐ l ‐arginine methyl ester thrombin chromogenic substrate d...
Use of plant extracts, alone or combined to the current chemotherapy as chemosensitizers, has emerged a promising strategy overcome tumor drug resistance. Here, we investigated anticancer activity Allium roseum L. wild edible species in North Africa, on human Chronic Myeloid Leukemia (CML) K562 cells. The dehydrated aqueous extract (DAE) disturbed cell cycle progression and induced apoptosis Chemical analysis DAE showed diversity organosulfur compounds S-alk(en)yl-cysteine sulfoxides (RCSO)...
The venoms of Viperidae snakes contain numerous serine proteinases that have been recognized to possess one or more the essential activities thrombin on fibrinogen and platelets. Among them, a platelet proaggregant protein, cerastocytin, has isolated from venom Tunisian viper Cerastes cerastes. Using RACE-PCR technique, we identified complete nucleotide sequence cDNA proteinase precursor. recombinant protein was designated rCC-PPP (for C. cerastes protein), since its deduced amino acid is...
The failure of chemotherapy especially in triple negative breast cancer (TNBC) patients has been correlated with the overexpression mesenchymal-epithelial transition factor (c-Met) receptor. Thus, hepatocyte growth (HGF)/c-Met signaling axis gained considerable attention as a valid molecular target for therapy. This study reports first time discovery 131 -oxophorbines pheophorbide A and protopheophorbide along chlorophyllide from Ziziphus lotus, an edible typical Tunisian plant, potent...
A new toxin, BotIT2, with a unique mode of action on the isolated giant axon cockroach Periplaneta americana and DUM (dorsal unpaired median) neurons, has been purified from venom scorpion Buthus occitanus tunetunus. Its structural, antigenic pharmacological properties are compared to those three other groups neurotoxins found in Buthidae venoms. Like excitatory, depressant α‐type insect‐selective neurotoxins, BotIT2 is toxic insects, but shows following common distinctive characteristics....
Two toxin‐like proteins (AahTL1 and AahTL3) were purified from the venom of scorpion Androctonus australis Hector (Aah). AahTL1 AahTL3 are first non toxic cross‐reacting with AahI toxins group which indicates that these can be used as a model vaccins. In order to study structure‐function relationships, their complete amino‐acid sequences (66 residues) determined, by automated Edman degradation. They show more than 50% similarity both AahIII antimammal toxins. Three‐dimensional structural...