A5003063897- Electrolyte and hormonal disorders
- Ion Transport and Channel Regulation
- Hormonal Regulation and Hypertension
- Renin-Angiotensin System Studies
- Apelin-related biomedical research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- Diet and metabolism studies
- Dialysis and Renal Disease Management
- Histone Deacetylase Inhibitors Research
- Acute Kidney Injury Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Adenosine and Purinergic Signaling
- Sodium Intake and Health
- Diabetes Treatment and Management
- Renal function and acid-base balance
- Cardiac Ischemia and Reperfusion
- Pancreatic function and diabetes
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Pediatric Urology and Nephrology Studies
- Metabolism and Genetic Disorders
- Nitric Oxide and Endothelin Effects
- Pharmacological Effects and Toxicity Studies
- Pregnancy and preeclampsia studies
Centre de Recherche des Cordeliers
2013-2023
Université Paris Cité
2012-2023
Sorbonne Université
2012-2023
Inserm
2012-2021
Délégation Paris 5
2011-2021
Université Sorbonne Nouvelle
2021
Metabolism and Renal Physiology
2018-2019
Centre National de la Recherche Scientifique
2001-2019
Sorbonne Paris Cité
2015-2018
Délégation Paris 7
2013-2015
This study tests the possible influence of urinary concentrating process and/or vasopressin (AVP) on progression early chronic renal failure (CRF). Male Sprague-Dawley rats were submitted to 5/6 nephrectomy and offered water ad libitum throughout study. In addition, half (high intake, HWI) received their food mixed with a water-rich agar gel. The other (normal NWI) ate same amount plus in usual dry powder form. resulted doubling daily ingestion HWI. Renal function was studied for 10 wk...
High plasma copeptin (copeptin), the C-terminal fragment of arginine vasopressin pro-hormone, has been associated with metabolic syndrome (MetS), diabetes mellitus (DM) development and nephropathy. Here we tested whether elevated level is later MetS, its individual components microalbuminuria. We analysed at baseline (1991–1994) in population-based Malmö Diet Cancer Study cardiovasular cohort re-examined 2064 subjects 15.8 years (mean age 72.8 years, 59% women) oral glucose tolerance test...
OBJECTIVE Water intake alters vasopressin secretion. Recent findings reveal an independent association between plasma copeptin, a surrogate for vasopressin, and risk of diabetes. RESEARCH DESIGN AND METHODS Participants were 3,615 middle-aged men women, with normal baseline fasting glycemia (FG), who recruited in 9-year follow-up study. Odds ratios (ORs) 95% CIs the incidence hyperglycemia (FG ≥6.1 mmol/L or treatment diabetes) calculated according to daily water classes based on...
Vasopressin plays a central role in water homeostasis but it has also been recognized to be associated with adverse effects several chronic diseases. Recently, copeptin increasingly used as surrogate for vasopressin, they are co-secreted, and is easier measure. However, the relationship between plasma concentrations of (P(cop)) vasopressin (P(vp)) only studied relatively small numbers selected people.This study sought evaluate P(vp) P(cop) community-based population people kidney disease...
Diabetic nephropathy is a major risk factor for end-stage renal disease and cardiovascular diseases has marked genetic component. A common variant (D allele) of the angiotensin Iconverting enzyme ( ACE ) gene, determining higher levels, been associated with diabetic nephropathy. To address causality underlying this association, we induced diabetes in mice having one, two, or three copies normal blood pressure, an level range (65–162% wild type) comparable to that seen humans. Twelve weeks...
Diabetic nephropathy represents a major complication of diabetes mellitus (DM), and the origin this is poorly understood. Vasopressin (VP), which elevated in type I II DM, has been shown to increase glomerular filtration rate normal rats contribute progression chronic renal failure 5/6 nephrectomized rats. The present study was thus designed evaluate whether VP contributes disorders DM. Renal function compared Brattleboro with insipidus (DI) lacking Long-Evans (LE) rats, or without...
In addition to its effect on water permeability, vasopressin, through V2 receptors (AVPR2), stimulates Na reabsorption in the collecting duct by increasing activity of amiloride-sensitive sodium channel ENaC. This study evaluated whether dDAVP (a potent AVPR2 agonist) reduces excretion healthy humans (n = 6) and patients with central (C; n 2) or nephrogenic (N) diabetes insipidus (DI) as a result mutations either aquaporin 2 gene (AQP2; 3) 10). was infused intravenously (0.3 μg/kg body wt 20...
Plasma copeptin, a surrogate for vasopressin, was associated with albuminuria in population-based studies. These associations are consistent the effect of vasopressin on observed humans and rodents. The objective this study to determine whether plasma copeptin is an independent marker risk renal events people type 2 diabetes albuminuria.We studied 3,101 participants DIABHYCAR trial (6-year follow-up) albuminuria. A event defined as doubling serum creatinine or development end-stage...
Experimental data support a role for vasopressin in metabolic disorders.We investigated associations of plasma copeptin, surrogate vasopressin, and allelic variations the arginine vasopressin-neurophysin II gene with insulin secretion, sensitivity, risk impaired fasting glucose (IFG) type 2 diabetes mellitus (T2DM).We studied 5110 unrelated French men women from prospective cohort general population (Data Epidemiological Study on Insulin Resistance Syndrome cohort, 9-y follow-up). Six single...
Vasopressin is known to acutely stimulate sodium transport in the renal collecting duct. We investigated long-term regulation by vasopressin of epithelial channel (ENaC) rat kidney. Five-day infusion dDAVP (a V 2 receptor agonist) Brattleboro rats lacking induced a marked increase β- and γ-subunit ENaC mRNA levels cortex (β, 85%; γ, 100%), with no change α-ENaC mRNA. Expression γ-ENaC mRNAs was also enhanced lung 49%; 33%) but not distal colon (an organ devoid receptors). Similar results...
To evaluate the possible influence of chronic alterations in urine concentrating activity (CA) on renal hemodynamics, adult male Sprague-Dawley rats were submitted for 7 days to one three different levels CA. CA was either reduced by increasing water intake (mixing food with a gel bringing 1.6 mL per g food) (Low-CA), or increased intraperitoneal infusion 1-desamino 8-D-arginine vasopressin (200 ng/day) (High-CA). Low-CA, High-CA, and control housed metabolic cages, ate same quantity dry...
The localization of two type 1 angiotensin II receptor subtype mRNA, AT1A and AT1B, was determined by reverse transcription-PCR on microdissected glomeruli nephron segments. coupling sensitivity these subtypes evaluated measuring variations in intracellular calcium ([Ca2+]i) elicited (Ang II) structures expressing either or AT1B using Fura-2 fluorescence. highest expression AT1 mRNA found glomerulus, proximal tubule, thick ascending limb. In were similarly expressed, whereas all segments...
The mechanisms by which arginine vasopressin (AVP) exerts its antidiuretic and pressor effects, via activation of V2 V1a receptors, respectively, are relatively well understood, but the possible associated effects on sodium handling a matter controversy. In this study, normal conscious Wistar rats were acutely administered various doses AVP, dDAVP (V2 agonist), furosemide, or following selective non-peptide receptor antagonists SR121463A antagonist) SR49059 (V1a antagonist). Urine flow...
Apelin receptors (ApelinRs) are expressed along an increasing cortico-medullary gradient in collecting ducts (CDs). We showed here that iv injection of apelin 17 (K17F) lactating rats characterized by increases both synthesis and release arginine vasopressin (AVP) increased diuresis concomitantly with a significant decrease urine osmolality no change Na(+) K(+) excretion. Under these conditions, we also observed apical aquaporin-2 immunolabeling CD, gradient, suggesting K17F-induced could be...
Plasma copeptin, a surrogate for vasopressin, has been associated with decline in renal function and albuminuria population-based studies as well progression of diabetic nephropathy people type 2 diabetes. We assessed the risk kidney coronary events all-cause mortality plasma copeptin 1 diabetes.Plasma was measured baseline samples GENEDIAB (n = 398; 56% male; mean ± SD age 45 12 years diabetes duration 28 10 years) GENESIS 588; 52% 42 11 years; 27 9 cohorts. Follow-up data were available...
High plasma copeptin, a marker of vasopressin, predicts diabetes mellitus. We tested if copeptin could be suppressed by increased water intake in healthy individuals, and water-induced change was accompanied altered concentrations glucose, insulin or glucagon.Thirty-nine individuals underwent, random order, 1 week high (3 L/day on top habitual intake) normal (habitual) fluid (control). Fasting glucagon were compared between the ends both periods. Furthermore, acute kinetics mapped for 4 h...