A5058540019- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Caveolin-1 and cellular processes
- Axon Guidance and Neuronal Signaling
- Cellular Mechanics and Interactions
- interferon and immune responses
- Glycosylation and Glycoproteins Research
- Optical Polarization and Ellipsometry
- Protease and Inhibitor Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- Immune Response and Inflammation
- Spectroscopy and Chemometric Analyses
- RNA Interference and Gene Delivery
- Genetic factors in colorectal cancer
- Inflammatory Bowel Disease
- Proteoglycans and glycosaminoglycans research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Platelet Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Wnt/β-catenin signaling in development and cancer
- Zebrafish Biomedical Research Applications
Université de Strasbourg
2014-2024
Inserm
2013-2024
Institut Polytechnique de Bordeaux
2022-2024
Biotechnologie et Signalisation Cellulaire
2022-2024
École Supérieure de Biotechnologie de Strasbourg
2024
Centre National de la Recherche Scientifique
2019-2022
Biopathologie de la myéline, neuroprotection et stratégies thérapeutiques
2019-2020
Institut de Biologie Moléculaire des Plantes
2019
Immuno-Rhumathologie moléculaire
2016
Levi Strauss (United States)
2015
Muscle stem cells (MuSCs) exhibit distinct behavior during successive phases of developmental myogenesis. However, how their transition to adulthood is regulated poorly understood. Here, we show that fetal MuSCs resist progenitor specification and altered division dynamics, intrinsic features are progressively lost postnatally. After transplantation, expand more efficiently contribute muscle repair. Conversely, niche colonization efficiency increases in adulthood, indicating a balance...
The extracellular matrix molecule tenascin-C (TNC) is a major component of the cancer-specific matrix, and high TNC expression linked to poor prognosis in several cancers.To provide comprehensive understanding TNC's functions cancer, we established an immune-competent transgenic mouse model pancreatic b-cell carcinogenesis with varying levels compared stochastic neuroendocrine tumor formation abundance or absence TNC.We show that promotes cell survival, angiogenic switch, more leaky vessels,...
Metastasis is a major cause of death in cancer patients. The extracellular matrix molecule tenascin-C known promoter metastasis, however the underlying mechanisms are not well understood. To further analyze impact on progression we generated MMTV-NeuNT mice that develop spontaneous mammary tumors, knockout background. We also developed syngeneic orthotopic model which tumor cells derived from tumor. Tumor were transfected with control shRNA or to knockdown expression and, grafted into gland...
Abstract Inherent immune suppression represents a major challenge in the treatment of human cancer. The extracellular matrix molecule tenascin-C promotes cancer by multiple mechanisms, yet roles tumor immunity are incompletely understood. Using 4NQO-induced oral squamous cell carcinoma (OSCC) model with abundant and absent tenascin-C, we demonstrated that enforced an immune-suppressive lymphoid stroma via CCL21/CCR7 signaling, leading to increased metastatic tumors. Through TLR4, expression...
Breast cancer is still a deadly disease despite major achievements in targeted therapies designed to block ligands or ligand-binding subunits of tyrosine kinase receptors. Relapse significant and metastases deleterious, which demands novel strategies for fighting this disease. Here, we report proof-of-concept experiment demonstrating that small peptides interfering with the transmembrane domain epidermal growth factor receptor ErbB2 exhibit anticancer properties when used at micromolar...
Laminins (LM), basement membrane molecules and mediators of epithelial-stromal communication, are crucial in tissue homeostasis. Inflammatory Bowel Diseases (IBD) multifactorial pathologies where the microenvironment particular LM play an important yet poorly understood role maintenance, cancer progression which represents inherent risk IBD. Here we showed first that human IBD colonic samples murine colitis LMα1 LMα5 chains specifically ectopically overexpressed with a concomitant nuclear...
The neuropilin-plexin receptor complex regulates tumor cell migration and proliferation thus is an interesting therapeutic target. High expression of neuropilin-1 indeed associated with a bad prognosis in glioma patients. Q-RTPCR tissue-array analyses showed here that Plexin-A1 highly expressed glioblastoma the highest level correlates worse survival We next identified developmental tumor-associated pro-angiogenic role Plexin-A1. Hence, by using molecular simulations two-hybrid like assay...
// Alexia Arpel 1, 2 , Coralie Gamper 1 Caroline Spenlé Aurore Fernandez Laurent Jacob Nadège Baumlin Patrice Laquerriere Gertraud Orend Gérard Crémel Dominique Bagnard INSERM U 1109, MN3T Laboratory, Labex Medalis, Strasbourg University, Strasbourg, France CNRS UMR 7178, Institut Pluridisciplinaire Hubert Curien, Correspondence to: Bagnard, email: bagnard@unistra.fr Keywords: neuropilin-1, breast cancer, metastasis, treatment, peptide Received: September 28, 2015 Accepted: May 2016...
Current treatments in multiple sclerosis (MS) are modulating the inflammatory component of disease, but no drugs currently available to repair lesions. Our study identifies MS patients overexpression Plexin-A1, signalling receptor oligodendrocyte inhibitor Semaphorin 3A. Using a novel type peptidic antagonist, we showed possibility counteract Sema3A inhibitory effect on migration and differentiation vitro when antagonizing Plexin-A1. The use this compound vivo demonstrated myelin protective...
The extracellular matrix (ECM) molecule tenascin-C (TNC) promotes tumor progression. This has recently been demonstrated in the stochastic murine RIP1-Tag2 insulinoma model, engineered to either express TNC abundantly or be devoid of TNC. However, our knowledge about organization microenvironment is scant. Here we determined spatial distribution together with other ECM molecules and human cancer tissue (insulinoma colorectal carcinoma). We found that organized tracks AngioMatrix signature, a...
Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease leading to demyelination axonal loss. Current treatments are immunomodulatory or immunosuppressive drugs acting on the inflammatory component. However, these do not adequately address crucial aspect of neuroprotection. Recently, association between altered balance adipokines MS has been proposed as both a risk factor for developing chronic aggravating factor. Specifically, decrease apelin plasma levels in patients...
Tumor-targeted therapies have often been inefficient due to the lack of concomitant control over tumor microenvironment. Using an immunocompetent autologous breast cancer model, we investigated a MAtrix REgulating MOtif (MAREMO)-mimicking peptide, which inhibits protumorigenic extracellular matrix (ECM) molecule tenascin-C that activates several hallmarks. In cultured cells, targeting MAREMO blocks signaling involved in cell adhesion and immune-suppression by inhibiting interactions with...
Laminins are major constituents of basement membranes and essential for tissue homeostasis. Laminin-511 is highly expressed in the intestine its absence causes severe malformation embryonic lethality. To understand mechanistic role laminin-511 homeostasis, we used RNA profiling intestinal lama5 knockout mice identified a specific gene expression signature. By combining cell culture experiments with mediated knockdown approaches, provide link between laminin α5 deficiency physiological...
Components with self-assembly properties derived from plant viruses provide the opportunity to design biological nanoscaffolds for ordered display of agents diverse nature and complementing functions. With aim designing a functionalized nanoscaffold target cancer, coat protein (CP) Tobacco mosaic virus (TMV) was tested as nanocarrier an insoluble, highly hydrophobic peptide that targets transmembrane domain Neuropilin-1 (NRP1) receptor in cancer cells. The resulting construct CPL-K...
Tumor stroma remodeling is a key feature of malignant tumors and can promote cancer progression. Laminins are major constituents basement membranes that physically separate the epithelium from underlying stroma.
Neuraminidases (NEUs) also called sialidases are glycosidases which catalyze the removal of terminal sialic acid residues from glycoproteins, glycolipids, and oligosaccharides. Mammalian NEU-1 participates in regulation cell surface receptors such as insulin receptor (IR), epithelial growth factor receptor, low-density lipoprotein toll-like 4. At plasma membrane, can be associated with elastin-binding protein carboxypeptidase protective protein/cathepsin A to constitute elastin complex. In...
Radiotherapy, the most frequent treatment of oral squamous cell carcinomas (OSCC) besides surgery is employed to kill tumor cells but, radiotherapy may also promote relapse where immune-suppressive microenvironment (TME) could be instrumental. We established a novel syngeneic grafting model from carcinogen-induced tongue tumor, OSCC13, address impact on OSCC. This revealed similarities with human OSCC, recapitulating mutations found in smoking associated tumors, abundant infiltrating...