A5035986364- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Insect behavior and control techniques
- Insect symbiosis and bacterial influences
- FOXO transcription factor regulation
- Optimism, Hope, and Well-being
- PI3K/AKT/mTOR signaling in cancer
- Quinazolinone synthesis and applications
- Biotechnology and Related Fields
- Cancer-related gene regulation
- Dietary Effects on Health
- Biological Research and Disease Studies
- Heat shock proteins research
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Protein Degradation and Inhibitors
- Aquaculture disease management and microbiota
- Lung Cancer Treatments and Mutations
- Diet and metabolism studies
- Circular RNAs in diseases
- Parasites and Host Interactions
- Hippo pathway signaling and YAP/TAZ
- Nutrition, Genetics, and Disease
- Physiological and biochemical adaptations
Kiel University
2015-2024
German Center for Lung Research
2021-2024
Institute of Molecular Biology
2023
Boston University
2023
Research Center Borstel - Leibniz Lung Center
2023
Zoological Institute
2016
Extensive remodeling of the airways is a major characteristic chronic inflammatory lung diseases such as asthma or obstructive pulmonary disease (COPD). To elucidate importance deregulated immune response in for processes, we established matching Drosophila model. Here, triggering Imd (immune deficiency) pathway tracheal cells induced organ-wide remodeling. This structural comprises disorganization epithelial structures and comprehensive thickening. We show that these changes do not depend...
Abstract Lung cancer is the leading cause of cancer-associated mortality. Mutations in EGFR gene are among most important inducers lung tumor development, but success personalized therapies still limited because toxicity or developing resistances. We expressed constitutively active (EGFRCA) exclusively airway system Drosophila melanogaster and performed comprehensive phenotyping. Ectopic expression EGFRCA induced massive hyper- metaplasia, to early death. used lethal phenotype as a readout...
Knowing the molecular makeup of an organ system is required for its in-depth understanding. We analyzed repertoire adult tracheal fruit fly Drosophila melanogaster using transcriptome studies to advance our knowledge insect system. Comparing this larval revealed several major differences that likely influence function. During transition from system, a shift in expression genes responsible formation cuticular structure occurs. This change transcript composition manifests physical properties...
Abstract The remarkable architecture of their respiratory system enables the unique metabolic performance that makes insects so successful. We performed single-cell RNAseq analysis larval tracheal Drosophila melanogaster and identified 17 cell populations are part or closely associated with trachea. In addition to several groups epithelial cells different progenitor types, secretory Inka showed unexpected properties. These express almost all molecular elements presynaptic active zone...
Abstract Sirtuins act as cellular sensors in the gut that control a substantial change properties response to environmental changes. Here we show only mitochondrial sirtuin of Drosophila, dSirt4 , is strongly up-regulated by protein-reduced diet. Flies with defect strong changes protein pattern and physiological their intestine. One most notable effects was induction lysozyme gene expression intestine, which also translates into enhanced activity. This effect cell autonomous, it observed...
Tumors of the intestinal tract are among most common tumor diseases in humans, but, like many other entities, show an unsatisfactory prognosis with a need for effective therapies. To test whether nutritional interventions and combination targeted therapy can effectively cure these cancers, we used fruit fly Drosophila as model. In this system, induced tumors by EGFR overexpression stem cells. Limiting amount protein diet restored life span to that control animals. specific inhibitor, all...
Diets that restrict caloric or protein intake offer a variety of benefits, including decreasing the incidence cancer. However, whether such diets pose substantial therapeutic benefit as auxiliary cancer treatments remains unclear. We determined effects severe depletion on tumorigenesis in <i>Drosophila melanogaster</i> intestinal tumor model, using human RAF gain-of-function allele. Severe and continuous restriction significantly reduced growth but resulted premature death. Therefore, we...
Abstract Airway epithelia have the challenging task of maintaining functional and structural homeostasis, even when exposed to various stress factors. Transcription factors FoxO family can fulfill this complex task, as they act integration hubs that translate extrinsic intrinsic information into a physiologically appropriate response. We could show in Drosophila , mouse, human airway epithelial cells (AECs) respond stressors like hypoxia, temperature, or oxidative by nuclear translocation. A...
Airway epithelia have the challenging task of maintaining functional and structural homeostasis, even when exposed to various stress factors. Transcription factors FoxO family can fulfill this complex task, as they act integration hubs that translate extrinsic intrinsic information into a physiologically appropriate response. We could show in Drosophila , mouse, human airway epithelial cells (AECs) respond stressors like hypoxia, temperature, or oxidative by nuclear translocation. A...
Airway epithelia have the challenging task of maintaining functional and structural homeostasis, even when exposed to various stress factors. Transcription factors FoxO family can fulfill this complex task, as they act integration hubs that translate extrinsic intrinsic information into a physiologically appropriate response. We could show in Drosophila , mouse, human airway epithelial cells (AECs) respond stressors like hypoxia, temperature, or oxidative by nuclear translocation. A...
Abstract Respiratory organs fulfill several tasks, of which gas exchange is the most important. This function also true for Drosophila respiratory organ, tracheal system, in terminal cells (TTCs), functional equivalents mammalian lung’s alveoli, are structures where occurs. To cope with plethora inhaled bacteria, fungi, and viruses, trachea, like all airway organs, developed a sophisticated innate immune system to protect its large surface area. Bacterial infection larval induced robust...
Respiratory organs fulfill several tasks, of which gas exchange is the most important. This function also true for Drosophila respiratory organ, tracheal system, in terminal cells (TTCs), functional equivalents mammalian lung’s alveoli, are structures where occurs. To cope with plethora inhaled bacteria, fungi, and viruses, trachea, like all airway organs, developed a sophisticated innate immune system to protect its large surface area. Bacterial infection larval induced robust response...
Respiratory organs fulfill several tasks, of which gas exchange is the most important. This function also true for Drosophila respiratory organ, tracheal system, in terminal cells (TTCs), functional equivalents mammalian lung’s alveoli, are structures where occurs. To cope with plethora inhaled bacteria, fungi, and viruses, trachea, like all airway organs, developed a sophisticated innate immune system to protect its large surface area. Bacterial infection larval induced robust response...
Drosophila nephrocytes are specialised cells that share critical functional, morphological, and molecular features with mammalian podocytes. Accordingly, represent a preferred invertebrate model for human glomerular disease. Here, we established method cell-specific isolation of the two types nephrocytes, garland pericardial cells, from animals different developmental stages ages. Mass spectrometry-based proteomics RNA-Seq-based transcriptomics were applied to characterise proteome...
Lung cancer remains the leading cause of cancer-associated mortality. Despite recent promising achievements, overall prognosis very poor. In order to integrate advantages adapted, transgenic animal models with a high-throughput procedure on one hand and compliance 3R principles other hand, we have established evaluated appropriate Drosophila models. To achieve this goal, ectopically expressed oncogenes representing most important driver mutations exclusively in airway system. These were...
Die Taufliege Drosophila melanogaster dient als eines der wichtigsten Modellsysteme in biomedizinischen Forschung. Sie zeichnet sich insbesondere durch einmalige Möglichkeiten genetischen Manipulation aus, wozu auch die Herstellung humanisierter Fliegen gehört. Wesentliche Erkenntnisse molekularen Grundlagen unterschiedlicher kanzerogener Erkrankungen konnten mithilfe des Modells gewonnen werden. Derzeit bestehen noch keine etablierten Lungenkarzinom-Modelle, eine Lücke, das hier...
Lung cancer is one of the most aggressive types with an extremely high overall fatality rate due to late state detection and lack treatment options. Drosophila a model for studying chronic inflammatory diseases lung, it's tracheal system shares comprehensive structural functional similarities human thus making it ideal molecular framework underlying lung development.
Abstract Diets that restrict caloric or protein intake offer a variety of benefits, including decreasing the incidence cancer. However, whether such diets pose substantial therapeutic benefit as auxiliary cancer treatments remains unclear. We determined effects severe depletion on tumorigenesis in Drosophila melanogaster intestinal tumor model, using human RAF gain-of-function allele. Severe and continuous restriction significantly reduced growth but resulted premature death. Therefore, we...
<div>Abstract<p>Lung cancer is the leading cause of cancer-associated mortality. Mutations in <i>EGFR</i> gene are among most important inducers lung tumor development, but success personalized therapies still limited because toxicity or developing resistances. We expressed constitutively active EGFR (EGFR<sup>CA</sup>) exclusively airway system <i>Drosophila melanogaster</i> and performed comprehensive phenotyping. Ectopic expression...