A5067831080- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Developmental Biology and Gene Regulation
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
Haverford College
2023-2024
European Molecular Biology Laboratory
2019
European Molecular Biology Laboratory
2019
University of Wisconsin–Madison
2010-2013
University of Oregon
2004-2007
Abstract Chloroplast genomes in land plants harbor ∼20 group II introns. Genetic approaches have identified proteins involved the splicing of many these introns, but to date cannot account for large size intron ribonucleoprotein complexes and are not sufficient reconstitute vitro. Here, we describe an additional protein that promotes chloroplast vivo. This protein, RNC1, was by mass spectrometry analysis maize (Zea mays) coimmunoprecipitate with two previously factors, CAF1 CAF2. RNC1 is a...
Sufficient amino acid supplies are critical for protein synthesis and, thus, cell growth and proliferation. Specialized transporters mediate exchange across membranes their regulation is homeostasis. Here, we report that the DNA- RNA-binding YBX3 regulates expression of transporters. To investigate functions YBX3, integrated proteomic transcriptomic data from cells depleted with analyses RNA binding sites to identify RNAs directly regulated by YBX3. The implicate as a distinct sets mRNAs...
Abstract Group II introns are ribozymes that catalyze a splicing reaction with the same chemical steps as spliceosome-mediated splicing. Many group have lost capacity to self-splice while acquiring compensatory interactions host-derived protein cofactors. Degenerate particularly abundant in organellar genomes of plants, where their requirement for nuclear-encoded factors provides means integration nuclear and functions. We present biochemical analysis between factor its chloroplast intron...
Regulatory complexes formed on mRNAs control translation, stability, and localization. These possess two activities: one that binds RNA another—the effector—that elicits a biological function. The Pumilio FBF (PUF) protein family of binding proteins provides versatile scaffold to design select with new specificities. Here, the PUF is used target translational activation repression specific mRNAs, induce poly(A) addition removal. To do so, we linked activator, GLD2, or repressor, CAF1....
Abstract Cancer-relevant signalling pathways rely on bidirectional nucleocytoplasmic transport events through the nuclear pore complex (NPC). However, mechanisms by which individual NPC components (Nups) participate in regulation of these remain poorly understood. We discover integrating large scale proteomics, polysome fractionation and a focused RNA i approach that Nup155 controls mRNA translation p21 ( CDKN1A ), key mediator p53 response. The underlying mechanism involves transcriptional...
The Xenopus Cripto-1 protein is confined to the cells of animal hemisphere during early embryogenesis where it regulates formation anterior structures. accumulates only in because cripto-1 mRNA vegetal translationally repressed. Here, we show that RNA binding protein, Bicaudal-C (Bic-C), functioned directly this cell-specific repression. While Bic-C normally cells, ectopic expression repressed a reporter and associated with endogenous mRNA. Repression by required its N-terminal domain,...
<h2>Abstract</h2> In humans, skeletal muscles comprise nearly 40% of total body mass, which is maintained throughout adulthood by a balance muscle protein synthesis and breakdown. Cellular amino acid (AA) levels are critical for these processes, mammalian cells contain transporter proteins that import AAs to maintain homeostasis. Until recently, the control regulation has largely been studied at transcriptional post-translational levels. However, here we report RNA-binding YBX3 sustain...