A5068270045- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Social Sciences and Policies
- Metabolism and Genetic Disorders
- Human Rights and Immigration
- Immigration and Intercultural Education
- RNA modifications and cancer
- Schizophrenia research and treatment
- Electroconvulsive Therapy Studies
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Blood Pressure and Hypertension Studies
- Aging, Health, and Disability
- scientometrics and bibliometrics research
- Cell death mechanisms and regulation
- Nanoparticle-Based Drug Delivery
- Lanthanide and Transition Metal Complexes
- Brazilian Legal Issues
- Digital Economy and Work Transformation
- Cancer, Lipids, and Metabolism
- Proteoglycans and glycosaminoglycans research
- Migration, Aging, and Tourism Studies
- Diagnosis and treatment of tuberculosis
- Palliative and Oncologic Care
Universidad de Zaragoza
2010-2024
Biocom
2020-2024
Instituto de Investigación Sanitaria Aragón
2022-2024
Instituto de Biocomputación y Física de Sistemas Complejos
2020-2023
Universidad de Los Andes
2023
Camilo José Cela University
2013-2014
Universidad Autónoma de Madrid
2013
Hospital Universitario Virgen Macarena
2011
Hospital Universitario Puerta del Mar
2010
The textbook description of mitochondrial respiratory complexes (RCs) views them as free-moving entities linked by the mobile carriers coenzyme Q (CoQ) and cytochrome c (cyt c). This model (known fluid model) is challenged proposal that all RCs except complex II can associate in supercomplexes (SCs). proposed SCs are respirasome (complexes I, III, IV), I III IV. role unclear, their existence debated. By genetic modulation interactions between IV, we show these associations define dedicated...
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity biochemical environment (such as pH and ionic strength) rapid yet not well-understood heat transfer mechanisms throughout cell. Here, we present unique system for intracellular temperature mapping fluorescence microscope (uncertainty 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated breast cancer MDA-MB468 cells. Two-dimensional (2D)...
The generation of temperature gradients on nanoparticles heated externally by a magnetic field is crucially important in hyperthermia therapy. But the intrinsic low heating power nanoparticles, at conditions allowed for human use, limitation that restricts general implementation technique. A promising alternative local intracellular hyperthermia, whereby cell death (by apoptosis, necroptosis, or other mechanisms) attained small amounts heat generated thermosensitive sites. However, few...
Mitochondrial function generates an important fraction of the heat that contributes to cellular and organismal temperature maintenance, but actual values this parameter reached in organelles is a matter debate. The studies addressing issue have reported divergent results: from detecting same as cell average or incubation temperature, increasing differences up 10 degrees above value. Theoretical calculations based on physical laws exclude possibility relevant gradients between mitochondria...
Alterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear ratio) appear associated with several systemic diseases, including primary disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn extracted from whole (WB) instead peripheral mononuclear cells or buffy coat may yield different results due present platelets. The aim this work is quantify contribution platelets [mtDNAcn(WB)] propose a correction formula estimate leukocytes'...
The existence of reliable mtDNA reference sequences for each species is great relevance in a variety fields, from phylogenetic and population genetics studies to pathogenetic determination variants humans or animal models mtDNA-linked diseases. We present compelling evidence the sequencing errors on current mouse sequence. This includes deletion full codon two genes, substitution one amino acid five occasions also involvement tRNA rRNA genes. conclusions are supported by: (i) re-sequencing...
Complex I (CI) is the largest enzyme of mammalian mitochondrial respiratory chain. The biogenesis complex a very process due to its large size and number subunits (45 subunits). situation further complicated fact that have double genomic origin, as seven them are encoded by DNA. Understanding assembly characterization involved factors has advanced much in last years. However, until now, key part process, is, how at which step mitochondrially CI (ND subunits) incorporated was not known....
Biocompatibility restrictions have limited the use of magnetic nanoparticles for hyperthermia therapy to iron oxides, namely magnetite (Fe3O4) and maghemite (γ-Fe2O3). However, there is yet another oxide phase that has not been considered so far, in spite its unique properties: ε-Fe2O3. Indeed, whereas Fe3O4 γ-Fe2O3 a relatively low coercivity, ε-Fe2O3 exhibits giant coercivity. In this report, heating power comparison with similar size (∼20 nm) was measured wide range field frequencies...
Abstract The Apoptosis-Inducing Factor (AIF) is a moonlighting flavoenzyme involved in the assembly of mitochondrial respiratory complexes healthy cells, but also able to trigger DNA cleavage and parthanatos. Upon apoptotic-stimuli, AIF redistributes from mitochondria nucleus, where upon association with other proteins such as endonuclease CypA histone H2AX, it proposed organize DNA–degradosome complex. In this work, we provide evidence for molecular complex well cooperative effects among...
About half of the mitochondrial DNA (mtDNA) mutations causing diseases in humans occur tRNA genes. Particularly intriguing are those pathogenic than can reach homoplasmy and yet show very different penetrance among patients. These scarce and, addition to their obvious interest for understanding human pathology, they be excellent experimental examples model evolution fixation mutations. To date, only source this type is We report here generation characterization first pathological mutation...
Complete sequencing of the mitochondrial genome 13 cell lines derived from a variety human cancers revealed nine novel DNA (mtDNA) variations. One them, m.6267G>A, is recurrent mutation that introduces Ala122Thr substitution in mitochondrially encoded cytochrome c oxidase I (MT-CO1): p.MT-CO1: (GenBank: NP_536845.1). Biochemical analysis original and transmitochondrial cybrids generated by transferring DNAs to common nuclear background, indicate (COX) activity, respiration, growth galactose...
Apoptosis-Inducing Factor (AIF) is a protein involved in mitochondrial electron transport chain assembly/stability and programmed cell death. The relevant role of this underlined because mutations altering AIF properties result acute pediatric mitochondriopathies tumor metastasis. By generating an original AIF-deficient mouse strain, study attempted to analyze, single paradigm, the cellular developmental metabolic consequences loss subsequent oxidative phosphorylation (OXPHOS) dysfunction....
The influence of culture substrate stiffness (in the kPa range) on chondrocyte behavior has been described. Here we describe response to variations in a soft range (2-20 Pa), as it may play role understanding cartilage physiopathology.We developed system for cell substrates with different elastic moduli using collagen hydrogels and evaluated chondrocytes after 2, 4, 7 days monolayer three-dimensional (3D) cultures. Experiments were performed normoxia hypoxia order effect low oxygen...
Multiprotein complexes of the mitochondrial electron transport chain form associations to generate supercomplexes. The relationship between tumor cell ability assemble supercomplexes, tumorigenesis and metastasis has not been studied thoroughly. metabolic differences L929dt cells, which lost matrix attachment MHC-I expression, their parental line L929, were analyzed. cells have lower capacity energy through OXPHOS respiratory than L929 cells. Most importantly, show defects in supercomplex...
As the last step of OXPHOS system, mitochondrial ATP synthase (or complex V) is responsible for production by using generated proton gradient, but also has an impact on other important functions linked to this system. Mutations either in V structural subunits, especially mtDNA-encoded ATP6 gene, or its assembly factors, are molecular cause a wide variety human diseases, most them classified as neurodegenerative disorders. The role alterations cancer development metastasis been postulated. In...
Objective We have carried out a bibliometric study on the scientific publications in relation to atypical or second-generation antipsychotic drugs (SGAs) South Korea. Methods With EMBASE and MEDLINE databases, we selected those made Korea whose title included descriptors atypic* (atypical*) antipsychotic*, clozapine, risperidone, olanzapine, ziprasidone, quetiapine, sertindole, aripiprazole, paliperidone, amisulpride, zotepine, asenapine, iloperidone, lurasidone, perospirone blonanserin....
Objective: We performed a bibliometric study on scientific publications atypical antipsychotic drugs (AADs) from Australia. Methods: Using the EMBASE and MEDLINE databases, we chose those documents produced in Australia between 1993 2011, whose title included descriptors atypic* (atypical*), antipsychotic*, second-generation clozapine, risperidone, olanzapine, ziprasidone, quetiapine, sertindole, aripiprazole, paliperidone, amisulpride, zotepine, asenapine, iloperidone, lurasidone,...
Background: This is a bibliometric study on the scientific papers of second generation (atypical) antipsychotic drugs (SGAs) from Taiwan. Methods: With EMBASE and MEDLINE databases, we chose published documents Taiwan with descriptors atypical*, antipsychotic*, second-generation clozapine, risperidone, olanzapine, ziprasidone, quetiapine, sertindole, aripiprazole, paliperidone, amisulpride, zotepine, asenapine, iloperidone lurasidone. We used indicators production dispersion, i.e., Price's...