A5101516425- Advanced oxidation water treatment
- Genetic factors in colorectal cancer
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced Photocatalysis Techniques
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- RNA modifications and cancer
- Adsorption and biosorption for pollutant removal
- Ultrasound and Cavitation Phenomena
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Fibroblast Growth Factor Research
- Molecular Biology Techniques and Applications
- Digestive system and related health
- Nutrition, Genetics, and Disease
- RNA Interference and Gene Delivery
Universidad de Antioquia
2014-2024
Hospital Universitario Puerta de Hierro Majadahonda
2007-2017
Fundación Ciencias Exactas y Naturales
2014-2015
Hospital Virgen de la Salud
2000-2011
Universidad Autónoma de Madrid
2010-2011
Universidad de León
2011
University of Concepción
2010
Centro de Investigación del Cáncer
2009
Brigham and Women's Hospital
2008
Protein Express (United States)
2008
By virtue of their accumulated genetic alterations, tumor cells may acquire vulnerabilities that create opportunities for therapeutic intervention. We have devised a massively parallel strategy screening short hairpin RNA (shRNA) collections stable loss-of-function phenotypes. assayed from 6000 to 20,000 shRNAs simultaneously identify genes important the proliferation and survival five cell lines derived human mammary tissue. Lethal common these targeted many known cell-cycle regulatory...
Tumor epithelial cells within a tumor coexist with complex microenvironment in which variety of interactions between its various components determine the behavior primary tumors. Cancer‐associated fibroblasts ( CAF ) and M 2 macrophages, characterized by high expression different markers, including α‐ SMA , FSP 1 FAP or CD 163 DCSIGN respectively, are involved malignancy In present study, above markers macrophages was analyzed using RT ‐ PCR immunohistochemistry normal mucosa tissue from...
Abstract MicroRNAs (miRNAs) are noncoding RNAs that regulate expression of target mRNAs and controlled by tumor suppressors oncogenes. Altered specific miRNAs in several types its association with poor prognosis parameters have been reported. Fewer data available on impact patients' survival. We studied the miR‐17‐5p , miR‐106a miR‐126 survival correlation levels their host gene TP53 alterations. assessed 110 colon cancer patients E2F1 EGFL7 quantitative real‐time RT‐PCR loss heterozygosity...
Abstract About 25–50% of women with Cowden disease, a syndrome associated germ‐line mutations the PTEN gene (at 10q23), develop breast cancer (BC), but have been found in only 5% sporadic BCs. However, 29–48% BCs display loss heterozygosity 10q23, and about 40% show decrease or absence protein levels at time diagnosis. Promoter hypermethylation has identified as an alternative mechanism tumor‐suppressor inactivation, its importance silencing BC is unknown. We investigated promoter 90...
E-cadherin (CDH1) gene expression is strictly regulated. The transcriptional factors SNAIL and ZEB1 are involved in its repression, whereas activation of vitamin D receptor (VDR) by induces transcription. We study the functional correlation SNAIL, CDH1, VDR genes examine their possible involvement colon cancer. these four was measured real time-PCR 114 patients with colorectal cancer, tumor characteristics were analyzed each patient. associated downregulation CDH1 (P<0.001) products. also...
KDM6B/JMJD3 is a histone H3 lysine demethylase with an important gene regulatory role in development and physiology. Here, we show that human JMJD3 expression induced by the active vitamin D metabolite 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) modulates action of this hormone. 1,25(OH)2D3 activates promoter increases level RNA cancer cells. upregulation was strictly dependent on receptor (VDR) abolished cycloheximide. In SW480-ADH colon cells, knockdown or inactive mutant fragment decreased...
Tumor‐derived exosomes mediate tumorigenesis by facilitating tumor growth, metastasis, development of drug resistance, and immunosuppression. However, little is known about the isolated from bronchoalveolar lavage (BAL) in patients with lung neoplasm. Exosomes plasma BAL 30 75 nontumor pathology were quantified acetylcholinesterase activity characterized Western Blot, Electron Microscopy, Nanoparticle Tracking Analysis. Differences exosome cargo analyzed miRNA quantitative PCR pooled samples...
This work studies the sonochemical degradation of a penicillinic antibiotic (oxacillin) in simulated pharmaceutical wastewater. High frequency ultrasound was applied to water containing combined with mannitol or calcium carbonate. In presence additives, oxacillin efficiently removed through action. For comparative purposes, photo-Fenton, TiO2 photocatalysis and electrochemical oxidation processes were also tested. Therefore, evolution its associated antimicrobial activity (AA) monitored. A...
Abstract ZEB1 and SNAIL repress CDH1 induce epithelial–mesenchymal transition (EMT). However, also activate or regulate other target genes in different ways. For instance, vitamin D receptor (VDR), which activates expression upon ligand binding, is repressed by but induced ZEB1. We examined whether the biological activity of colon cancer regulated interacting cofactors. The mRNA levels , transcriptional regulators p300 CtBP, were measured RT‐PCR tumor normal tissue from 101 carcinoma...
Tumor-derived exosomes are emerging as local and systemic cell-to-cell mediators of oncogenic information through the horizontal transfer mRNAs, microRNAs proteins during tumorigenesis. The exosomal content has been described biologically active when taken up by recipient cell. Identifying specific molecular cargo will help to determine their function in steps tumorigenic process. Here we evaluate whether ΔNp73 is selectively packaged tumor-derived exosomes, its acceptor cells vitro vivo...