A5001432358- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- ATP Synthase and ATPases Research
- Metabolism, Diabetes, and Cancer
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- RNA modifications and cancer
- Iron oxide chemistry and applications
- Respiratory Support and Mechanisms
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Immune cells in cancer
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Biochemical and Molecular Research
- Lung Cancer Treatments and Mutations
- Pancreatic function and diabetes
- Toxoplasma gondii Research Studies
- Cancer Mechanisms and Therapy
- Long-Term Effects of COVID-19
- Ferroptosis and cancer prognosis
- Radiation Therapy and Dosimetry
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Characterization and Applications of Magnetic Nanoparticles
Universidad de Zaragoza
2019-2024
Instituto de Investigación Sanitaria Aragón
2019-2024
Université de Montpellier
2021
Immunité et Cancer
2021
Inserm
2021
Hospital Universitario de Fuenlabrada
2021
Biocom
2020
Instituto de Biocomputación y Física de Sistemas Complejos
2020
The generation of temperature gradients on nanoparticles heated externally by a magnetic field is crucially important in hyperthermia therapy. But the intrinsic low heating power nanoparticles, at conditions allowed for human use, limitation that restricts general implementation technique. A promising alternative local intracellular hyperthermia, whereby cell death (by apoptosis, necroptosis, or other mechanisms) attained small amounts heat generated thermosensitive sites. However, few...
Mitochondrial function generates an important fraction of the heat that contributes to cellular and organismal temperature maintenance, but actual values this parameter reached in organelles is a matter debate. The studies addressing issue have reported divergent results: from detecting same as cell average or incubation temperature, increasing differences up 10 degrees above value. Theoretical calculations based on physical laws exclude possibility relevant gradients between mitochondria...
Biocompatibility restrictions have limited the use of magnetic nanoparticles for hyperthermia therapy to iron oxides, namely magnetite (Fe3O4) and maghemite (γ-Fe2O3). However, there is yet another oxide phase that has not been considered so far, in spite its unique properties: ε-Fe2O3. Indeed, whereas Fe3O4 γ-Fe2O3 a relatively low coercivity, ε-Fe2O3 exhibits giant coercivity. In this report, heating power comparison with similar size (∼20 nm) was measured wide range field frequencies...
Leukemic cells proliferate faster than non-transformed counterparts. This requires them to change their metabolism adapt high growth. can stress and facilitate recognition by immune such as cytotoxic lymphocytes, which express the activating receptor Natural Killer G2-D (NKG2D). The tumor suppressor gene p53 regulates cell metabolism, but its role in expression of metabolism-induced ligands, subsequent is unknown. We show here that dichloroacetate (DCA), induces oxidative phosphorylation...
Abstract Solid tumor cells have an altered metabolism that can protect them from cytotoxic lymphocytes. The anti-diabetic drug metformin modifies cell and several clinical trials are testing its effectiveness for the treatment of solid cancers. use in hematologic cancers has received much less attention, although allogeneic lymphocytes very effective against these tumors. We show here induces expression Natural Killer G2-D (NKG2D) ligands (NKG2DL) intercellular adhesion molecule-1 (ICAM-1),...
Adoptive transfer of allogeneic natural killer (NK) cells is becoming a credible immunotherapy for hematological malignancies. In the present work, using an optimized expansion/activation protocol human NK cells, we generate expanded (eNK) with increased expression CD56 and NKp44, while maintaining that CD16. These eNK exerted significant cytotoxicity against from 34 B-CLL patients, only 1 sample exhibiting resistance. This sporadic resistance did not correlate match between KIR ligands...
Multiprotein complexes of the mitochondrial electron transport chain form associations to generate supercomplexes. The relationship between tumor cell ability assemble supercomplexes, tumorigenesis and metastasis has not been studied thoroughly. metabolic differences L929dt cells, which lost matrix attachment MHC-I expression, their parental line L929, were analyzed. cells have lower capacity energy through OXPHOS respiratory than L929 cells. Most importantly, show defects in supercomplex...
As the last step of OXPHOS system, mitochondrial ATP synthase (or complex V) is responsible for production by using generated proton gradient, but also has an impact on other important functions linked to this system. Mutations either in V structural subunits, especially mtDNA-encoded ATP6 gene, or its assembly factors, are molecular cause a wide variety human diseases, most them classified as neurodegenerative disorders. The role alterations cancer development metastasis been postulated. In...
In recent years, the number of studies dedicated to ascertaining connection between mitochondria and cancer has significantly risen. However, more efforts are still needed fully understand link involving alterations in tumorigenesis, as well identify tumor-associated mitochondrial phenotypes. For instance, evaluate contribution tumorigenesis metastasis processes, it is essential influence from tumor cells different nuclear environments. this purpose, one possible approach consists...
Cancer resistance to treatments is a challenge that researchers constantly seek overcome. For instance, TNF-related apoptosis-inducing ligand (TRAIL) potential good prospect as an anti-cancer therapy, it attacks tumor cells but not normal cells. However, based in soluble TRAIL provided incomplete clinical results and diverse formulations have been developed improve its bioactivity. In previous works, we generated new formulation attachment the surface of unilamellar nanoliposomes...
Intercellular communication between monocytes/macrophages and cells involved in tissue regeneration, such as mesenchymal stromal (MSCs) primary cells, is essential for regeneration recovery of homeostasis. Typically, the final phase inflammation-resolving process, this intercellular drives an anti-inflammatory immunomodulatory response. To obtain a safe effective treatment to counteract cytokine storm associated with disproportionate immune response severe infections, including that...
PT-112 is a novel pyrophosphate-platinum conjugate, with clinical activity reported in advanced pretreated solid tumors. While has been shown to induce robust immunogenic cell death (ICD) vivo but only minimally bind DNA, the molecular mechanism underlying target disruption cancer cells still under elucidation. The murine L929 vitro system was used test whether differential metabolic status alters PT-112's effects, including cytotoxicity. results showed that tumor presenting mutations...
PT-112 is a novel immunogenic cell death (ICD)-inducing small molecule currently under Phase 2 clinical development, including in metastatic castration-resistant prostate cancer (mCRPC), an immunologically cold and heterogeneous disease state need of therapeutic approaches. has been shown to cause ribosome biogenesis inhibition organelle stress followed by ICD cells, culminating anticancer immunity. In addition, evidence PT-112-driven immune effects observed patient immunoprofiling. Given...
Our research group has been developing a series of biological drugs produced by coculture techniques with M2-polarized macrophages different primary tissue cells and/or mesenchymal stromal (MSC), generally from fat, to produce anti-inflammatory and anti-fibrotic effects, avoiding the overexpression pro-inflammatory cytokines innate immune system at given time. One these products is drug PRS CK STORM, medium conditioned allogenic macrophages, coculture, those M2 MSC whose composition, in...
Solid tumor cells have an altered metabolism that can protect them from cytotoxic lymphocytes. The antidiabetic drug metformin modifies cell and several clinical trials are testing its effectiveness for the treatment of solid cancers. use in hematologic cancers has received much less attention, although allogeneic lymphocytes very effective against these tumors. We show here induces expression Natural Killer G2-D (NKG2D) ligands (NKG2DL) intercellular adhesion molecule-1 (ICAM-1), a ligand...
Nine kDa granulysin (GRNLY) is a human cytolytic protein secreted by cytotoxic T lymphocytes (CTL) and NK cells of the immune system whose demonstrated physiological function elimination bacteria parasites. In previous studies our group, anti-tumor capacity recombinant was demonstrated, both in vitro vivo. present work, we developed lipid nanoparticles surfaces can bind through formation complex coordination between histidine tail Ni2+ provided chelating liposome composition termed them...
TNF-related apoptosis-inducing ligand (TRAIL) is known to induce caspase-dependent apoptosis in tumor cells, while sparing normal cells. However, TRAIL also able proliferation cells that are resistant cell death induction, since it can activate NF-κB-dependent signaling pathways. Human mesenchymal stem (hMSC) transfected with have been used treat cancer, promising results pre-clinical models, especially gliomas.
Abstract Solid tumor cells have an altered metabolism that can protect them from cytotoxic lymphocytes. The antidiabetic drug metformin modifies cell and several clinical trials are testing its effectiveness for the treatment of solid cancers. use in hematologic cancers has received much less attention, although allogeneic lymphocytes very effective against these tumors. We show here induces expression Natural Killer G2-D (NKG2D) ligands (NKG2DL) intercellular adhesion molecule-1 (ICAM-1), a...
In recent years, the number of studies dedicated to ascertaining connection between mitochondria and cancer has significantly risen. However, more efforts are still needed fully understand link involving alterations in tumorigenesis, as well identify tumor-associated mitochondrial phenotypes. For instance, evaluate contribution tumorigenesis metastasis processes, it is essential influence from tumor cells different nuclear environments. this purpose, one possible approach consists...