A5087979607- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Immune cells in cancer
- Long-Term Effects of COVID-19
- Immune responses and vaccinations
- Inflammatory Biomarkers in Disease Prognosis
- Single-cell and spatial transcriptomics
- S100 Proteins and Annexins
- Advanced Fluorescence Microscopy Techniques
- Animal Disease Management and Epidemiology
- Advanced Biosensing Techniques and Applications
- Inflammatory Bowel Disease
- Vasculitis and related conditions
- HIV Research and Treatment
- Escherichia coli research studies
- Eosinophilic Disorders and Syndromes
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Asthma and respiratory diseases
- Cardiovascular Disease and Adiposity
- Plant Molecular Biology Research
- Inflammasome and immune disorders
University of Oxford
2020-2024
University Medical Center Utrecht
2017-2022
Cardiff University
2021
Kennedy Center
2020
Institute of Rheumatology
2020
Oxfam
2020
The University of Queensland
2012
Utrecht University
2012
Syngenta (China)
2003
A novel bacterium-free approach for rapid assembly of flavivirus infectious cDNAs using circular polymerase extension reaction was applied to generate cDNA the virulent New South Wales isolate Kunjin strain West Nile virus (KUNV) that recently emerged in Australia. Recovered recapitulated genetic heterogeneity present original isolate. The utilized viral mutants with designed phenotypic properties and identify E protein glycosylation as one virulence determinants.
Giant cell arteritis (GCA) is a common form of primary systemic vasculitis in adults, with no reliable indicators prognosis or treatment responses. We used single technologies to comprehensively map immune populations the blood patients GCA and identified CD66b+CD15+CD10lo/–CD64– band neutrophils CD66bhiCD15+CD10lo/–CD64+/bright myelocytes/metamyelocytes be unequivocally associated both clinical phenotype response treatment. Immature were resistant apoptosis, remained vasculature for...
At homeostasis the vast majority of neutrophils in circulation expresses CD16 and CD62L within a narrow expression range, but this quickly changes disease. Little is known regarding kinetics phenotypes inflammatory conditions. During acute inflammation more heterogeneity was found, characterized by an increase dim banded neutrophils. These cells were probably released from bone marrow (left shift). Acute induced human experimental endotoxemia (LPS model) additionally accompanied immediate...
Abstract Acute inflammation recruits neutrophils with a band-shaped nucleus to the circulation. This neutrophil population was recently shown have superior antibacterial capacity. Early recruitment of banded an infection site will likely improve outcome immune response, yet it critically depends on efficient migration. However, current dogma states that segmentation mature has evolved favor migration through narrow pores as found between endothelial cells and in interstitium. Therefore, we...
The first immune-activating changes within joint resident cells that lead to pathogenic leukocyte recruitment during articular inflammation remain largely unknown. In this study, we employ state-of-the-art confocal microscopy and image analysis in a systemic, whole-organ, quantitative way present evidence synovial begins with the activation of lining macrophages. We show lining, but not sublining macrophages phagocytose immune complexes containing model antigen. Using antigen-induced...
an uncorrected Dunn test.Statistically significant differences are shown in bold.
Improved knowledge of different biomarkers is crucial for early diagnosis rheumatic diseases and to provide important insights clinical management. In this study, we evaluated the seroreactivity patients with connective tissue (CTDs) (rheumatoid arthritis, RA; systemic lupus erythematosus, SLE; sclerosis, SSc; Sjogren's syndrome, SSj) interferon regulatory factor 5 (IRF5) peptide homologs derived from Epstein-Barr virus (EBV) Mycobacterium avium subsp. paratuberculosis (MAP). Antigen-induced...
Neutrophil antibacterial capacity is measured in animal models and vitro as an important indicator of neutrophil function. To be able to extrapolate their conclusions, experiments should mimic the vivo situation. In vivo, depends on multiple steps bacterial sensing, priming, chemotaxis, phagocytosis intracellular killing. Therefore, we developed a simply executed assay that involves one assay. The neutrophils were incorporated into three-dimensional matrix fibrin fibers, which they could...
During development neutrophils undergo extensive changes in nuclear morphology, gene expression programmes, and acquire specific effector functions. However, molecular mechanisms controlling neutrophil remain largely unknown. Here we systematically analysed chromatin landscape across stages of differentiation, to construct a network transcription factors, predicted control acquisition Zinc finger protein 263 (Zfp263) emerged at the apex this network. Neutrophil maturation ex vivo was blocked...
Abstract Neutrophils are innate immune cells that key to protecting the host against infection and maintaining body homeostasis. However, if dysregulated, they can contribute disease, such as in cancer or chronic autoinflammatory disorders. Recent studies have highlighted heterogeneity neutrophil compartment identified presence of immature neutrophils their precursors these pathologies. Therefore, understanding maturity mechanisms through which disease is critical. were first characterized...
Abstract Systemic inflammation (SI) plays a detrimental role in various conditions with high mortality rates 1–4 . SI manifests an acute hyperinflammation followed by long-lasting immunosuppression, increasing patients’ risks for secondary infections and impaired clinical outcomes 5–7 Due to the extensive heterogeneity etiology, mechanisms governing these states are incompletely understood. Here, we characterized late effects of lipopolysaccharide (LPS)-induced (LPS-SI 8 ) on blood monocytes...