A5105100058- Immune cells in cancer
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
Inserm
2024
Institut Gustave Roussy
2024
Université Paris-Saclay
2024
Tumor-associated macrophages (TAMs) are a heterogeneous population of cells whose phenotypes and functions shaped by factors that incompletely understood. Herein, we asked when where TAMs arise from blood monocytes how they evolve during tumor development. We initiated pancreatic ductal adenocarcinoma (PDAC) in inducible monocyte fate-mapping mice combined single-cell transcriptomics high-dimensional flow cytometry to profile the monocyte-to-TAM transition. revealed differentiate first into...
Macrophages orchestrate tissue homeostasis and immunity. In the tumor microenvironment (TME), macrophage presence is largely associated with poor prognosis because of their reprogramming into immunosuppressive cells. We investigated effects hypoxia, a TME-associated feature, on functional, epigenetic, transcriptional macrophages found that hypoxia boosts immunogenicity. Hypoxic inflammatory are characterized by cluster proinflammatory genes undergoing ten-eleven translocation–mediated DNA...