A5006722745- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Renal and related cancers
- Cardiac Ischemia and Reperfusion
- Connexins and lens biology
- Microtubule and mitosis dynamics
- PARP inhibition in cancer therapy
- Advanced MRI Techniques and Applications
- Cancer Mechanisms and Therapy
- S100 Proteins and Annexins
- Microbial Metabolic Engineering and Bioproduction
- Lipid Membrane Structure and Behavior
- Cancer Genomics and Diagnostics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genomics, phytochemicals, and oxidative stress
- Cellular transport and secretion
- Pregnancy and preeclampsia studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Gut microbiota and health
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced Glycation End Products research
- Image and Video Quality Assessment
- Aluminum Alloys Composites Properties
Wuhan University
2015-2025
Renmin Hospital of Wuhan University
2023-2025
Zhejiang University
2024
Stomatology Hospital
2024
Yangzhou University
2024
Jiangnan University
2024
National University of Singapore
2018-2022
Institute of Molecular and Cell Biology
2021-2022
Zhejiang Provincial Hospital of TCM
2019-2022
Agency for Science, Technology and Research
2021
Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine cilia, axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model 48-nm repeat a mammalian DMT, derived from cryoelectron microscopy (cryo-EM) map complex isolated bovine respiratory cilia. The structure uncovers principles organization features specific to vertebrate including previously unknown MIPs, bundle tektin...
Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain spine disorders. The development of ciliated cells, their impact on CSF flow, function in axial morphogenesis are not fully understood. We have characterized motile cells within the zebrafish ventricles. show that ventricles undergo restructuring through development, involving a transition from mono- to multiciliated (MCCs) driven by gmnc. MCCs co-exist with monociliated generate directional patterns. These...
Alveolar macrophages (AMs) are critical for lung immune defense and homeostasis. They orchestrators of chronic obstructive pulmonary disease (COPD), with their number significantly increased functions altered in COPD. However, it is unclear how AM function controlled a healthy if changes AMs without environmental assault sufficient to trigger inflammation We report here that absence isthmin 1 (ISM1) mice (Ism1-/- ) leads increase both functional heterogeneity, enduring inflammation,...
A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward centrosome forms "zygotene bouquet." Here, we identified cilium" in oocytes. This cilium provides a cable system for bouquet machinery extends throughout germline cyst. Using zebrafish mutants live manipulations, demonstrate that anchors to counterbalance pulling. The essential synaptonemal...
PURPOSE: To investigate if oxymatrine pretreatment could ameliorate renal I/R injury induced in rats and explore the possible role of Nrf2/HO-1 pathway. METHODS: Unilaterally nephrectomized were insulted by their left kidney. Twenty four randomly divided into three groups: sham group, + saline-treated OMT-treated group. Oxymatrine or vehicle solution was administered intraperitoneally injected 60 min before ischemia, respectively. Renal function, histology, makers oxidative stress, cell...
To investigate the effect of metformin on renal tubular epithelial cell apoptosis and inflammation after kidney ischemia/ reperfusion in rats.Eighteen SD rats were randomly divided into three groups: Sham (S), Ischemia/reperfusion (I/R), Metformin (E). Before establishing I/R model, group E was administered for days, while groups S equal volumes saline. After a right nephrectomy performed all groups, which left kidneys subjected to 45 min ischemia. Renal function, histology, assessed. AMPK,...
Classic endocytosis destinations include the recycling endosome returning to plasma membrane or late (LE) merging with lysosomes for cargo degradation. However, anti-angiogenic proteins angiostatin and isthmin, are endocytosed trafficked mitochondria (Mito) execute apoptosis of endothelial cells. How these extracellular reach remains a mystery. Through confocal super-resolution fluorescent microscopy, we demonstrate that isthmin through interaction between LE Mito. Using purified organelles,...
Patchoulol is a natural sesquiterpene alcohol with extensive applications in cosmetics and pharmaceuticals. In this study, we first constructed the synthesis pathway of patchoulol Saccharomyces cerevisiae by expressing synthase PTS gene using strong promoter GAL1. Afterward, metabolic flux precursor was enhanced strengthening mevalonate balancing competition pathway, resulting 32.74-fold increase production. Subsequently, supply acetyl-CoA yeast increased modifying transcriptional regulators...
Background: Hepatic ischemia-reperfusion injury (HIRI) stands as an unavoidable complication arising from liver surgery, profoundly intertwined with its prognosis. The role of lysine methyltransferase SET domain bifurcated 1 (SETDB1) in HIRI remains elusive, despite confirmation a potential therapeutic target for diverse diseases. Here, we investigated the mechanism by which SETDB1 regulated HIRI. Methods: RNA sequencing data were used to identify expression and targets through...
Numerous studies have demonstrated that microRNAs (miRNAs or miRs) play an important role in regulating osteogenic differentiation, but their specific regulatory mechanism requires further investigation. In the present study, it was revealed during differentiation of rat bone marrow mesenchymal stem cells (BMSCs), expression level miR-144-3p decreased with increased induction duration and negatively associated marker gene expression. Overexpression inhibited while inhibition promoted...
Expression of Ca2+/CaM‑dependent protein kinase II (CaMKII) and connexin 43 (Cx43) in a rat model post‑stroke depression (PSD) was investigated. Rats were separated into control group (10 rats underwent sham operation not ligated after incision), PSD (13 rats) KN93 (12 treated with KN93, an inhibitor CaMKII, on the basis group). After modeling, Longa scoring performed, open field test as well step‑through carried out to observe behavior. RT‑qPCR western blot analysis used detect expression...
ABSTRACT Motile cilia defects impair cerebrospinal fluid (CSF) flow, and can cause brain spine disorders. To date, the development of ciliated cells, their impact on CSF flow function in axial morphogenesis are not fully understood. Here, we have characterized motile cells within zebrafish ventricles. We show that ventricular surface undergoes significant restructuring through development, involving a transition from mono- to multiciliated (MCCs) driven by gmnc. MCCs translationally...
<title>Abstract</title> Background Renal ischemia-reperfusion injury (RIRI) stands as an unavoidable complication arising from kidney surgery, profoundly intertwined with its prognosis. The role of differentially expressed in FDCP 6 homolog (DEF6) RIRI remains elusive, despite confirmation a potential therapeutic target for diverse diseases. Here, we investigated the mechanism by which DEF6 regulated RIRI. Methods RNA sequencing data and IP-MS were used to identify expression targets through...
<title>Abstract</title> Background Alterations in the intestinal flora composition can influence host inflammation, metabolism, and immune response. While extensive research has explored gut dysbiosis various pituitary adenomas (PA), changes microbiota their correlations with clinical parameters prolactin-secreting adenoma (PPA) patients remain unknown. This study investigates these alterations associations explores microbial markers for PPA diagnosis. Methods A total of 101 participants...
Abstract The hallmark of meiosis is chromosomal pairing and synapsis via synaptonemal complexes, but also depends on cytoplasmic counterparts that tether rotate telomeres the nuclear envelope. Telomeres slide perinuclear microtubules, shuffling chromosomes mechanically driving their homology searches. Pull towards centrosome drives formation “zygotene bouquet”. These telomere dynamics are essential for fertility, bouquet, discovered in 1900, universally conserved. Nevertheless, how bouquet...
Objective: To evaluate the efficacy and safety of fibrinolysis strategy in patients with acute ST-segment elevation myocardial infarction (STEMI) during COVID-19 epidemic, to provide reference value for optimization fibrinolytic process on premise prevention control transmission, including self-protection medical staff. Methods: The were retrospectively analyzed 7 STEM, who hospitalized from February 29, 2020 April 3, Department Cardiology, Wuhan Union Hospital Tongji Medical College,...
The existing disparity map based virtual view rendering may decrease the sharpness of rendered image, or result in ghosts. In this paper, a new method is proposed. left and right images their corresponding maps are used to render intermediate image respectively. Then two resulted from references fused, remained holes interpolated with neighbouring pixels holes. Experimental results show that proposed can achieve high object quality as well PSNR image.
Compared to traditional multi-view systems, client-oriented video system could significantly simplify the complexity at client since part of disparity estimation is left sever. This paper proposes a fast refinement algorithm for system. Firstly, coarse map generated by adopting 8times8 block based on smoothness restriction server, and transmitted client. Then according spatial correlation vectors discontinuity boundary area objects, pixel-wise obtained with algorithm. Experimental results...