A5071711307- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Hemoglobin structure and function
- Genetic and Kidney Cyst Diseases
- Mitochondrial Function and Pathology
- Tuberculosis Research and Epidemiology
- Genetic Syndromes and Imprinting
- Diabetes and associated disorders
- Protist diversity and phylogeny
- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
- COVID-19 Impact on Reproduction
- Hedgehog Signaling Pathway Studies
- Adenosine and Purinergic Signaling
- Ion channel regulation and function
- Plant Stress Responses and Tolerance
- Biotin and Related Studies
- Cellular transport and secretion
- Circular RNAs in diseases
- Infrared Target Detection Methodologies
- Marine and coastal ecosystems
- Advanced Memory and Neural Computing
- Algal biology and biofuel production
- Pneumocystis jirovecii pneumonia detection and treatment
- Generative Adversarial Networks and Image Synthesis
University of Virginia
2023-2025
Rayat Bahra University
2024
Institute of Microbial Technology
2009-2023
Harvard University
2019-2021
Boston VA Research Institute
2020
Purdue University West Lafayette
2015-2020
Council of Scientific and Industrial Research
2012-2014
Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine cilia, axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model 48-nm repeat a mammalian DMT, derived from cryoelectron microscopy (cryo-EM) map complex isolated bovine respiratory cilia. The structure uncovers principles organization features specific to vertebrate including previously unknown MIPs, bundle tektin...
Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into BBSome, key regulator of ciliary membrane proteome. We report electron cryomicroscopy (cryo-EM) structures native bovine BBSome in inactive and active states at 3.1 3.5 Å resolution, respectively. In state, bound an Arf-family GTPase (ARL6/BBS3) that recruits membranes. ARL6 recognizes...
Abstract Enteroendocrine cells (EECs) are often dysregulated in metabolic and gastrointestinal diseases, such as inflammatory bowel disease, although their roles disease pathogenesis remain unknown. EECs rare sensory scattered throughout the epithelium that respond to environmental stimuli like nutrients microbes by secreting over 20 distinct hormones, neurotransmitters, metabolites. well-known for systemic effects regulating endocrine exocrine pancreas function appetite, but they play...
Chimeric RNAs from chromosomal rearrangements have long been validated as cancer markers and therapeutic targets for many years. Recently, trans-splicing cis-splicing between adjacent genes are also shown to generate chimeric RNAs. They influence tumor progression by coding fusion proteins, acting noncoding or circular RNAs, altering parental gene expression. Here, we analyzed The Cancer Genome Atlas Chinese Prostate Epigenome Atlas, identifying similarities differences Western prostate...
Mycobacterium tuberculosis (Mtb) is a phenomenally successful human pathogen having evolved mechanisms that allow it to survive within the hazardous environment of macrophages and establish long term, persistent infection in host against control cell-mediated immunity. One such mechanism mediated by truncated hemoglobin, HbN, Mtb displays potent O2-dependent nitric oxide dioxygenase activity protects its from toxicity macrophage-generated (NO). Here we demonstrate for first time HbN...
Oxygenic respiration and photosynthesis based on quinone redox reactions face a danger of wasteful energy dissipation by diversion the productive electron transfer pathway through generation reactive oxygen species (ROS). Nevertheless, widespread oxido-reductases from cytochrome bc family limit amounts released ROS to low, perhaps just signaling, level an as-yet-unknown mechanism. Here, we propose that metastable radical state, nonreactive with oxygen, safely holds electrons at local...
The truncated hemoglobin N, HbN, of Mycobacterium tuberculosis is endowed with a potent nitric oxide dioxygenase (NOD) activity that allows it to relieve nitrosative stress and enhance in vivo survival its host. Despite small size, the protein matrix HbN hosts two-branched tunnel, consisting orthogonal short long channels, connects heme active site surface. A novel dual-path mechanism has been suggested drive migration O2 NO distal cavity. While oxygen migrates mainly by path, ligand-induced...
The bacterial haemoglobin from Vitreoscilla, VHb, displays several unusual properties that are unique among the globin family. When gene encoding vgb, is expressed its natural promoter in either Vitreoscilla or Escherichia coli, level of VHb increases more than 50-fold under hypoxic conditions and decreases significantly during oxidative stress, suggesting similar functioning vgb both organisms. In present study we show expression E. coli induced antioxidant genes katG (catalase-peroxidase...
Many pathogenic microorganisms have evolved hemoglobin-mediated nitric oxide (NO) detoxification mechanisms, where a globin domain in conjunction with partner reductase catalyzes the conversion of toxic NO to innocuous nitrate. The truncated hemoglobin HbN Mycobacterium tuberculosis displays potent dioxygenase activity despite lacking domain. mechanism by which recycles itself during dioxygenation and that participates this process are currently unknown. This study demonstrates...
Several ‘super-complexes’ of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer harvesting light energy, have been previously characterized in the mitochondrial cristae chloroplast thylakoid membranes. We report presence an super-complex dominated by ATP-synthase, photosystem I (PSI) reaction-center complex ferredoxin-NADP+ Reductase (FNR) membrane. The has documented mass spectrometry, clear-native PAGE...
Aims: Although the human pathogen, Mycobacterium tuberculosis (Mtb), is strictly aerobic and requires efficient supply of oxygen, it can survive long stretches severe hypoxia. The mechanism responsible for this metabolic flexibility unknown. We have investigated a novel by which hemoglobin O (HbO), operates supports its host under oxygen stress. Results: discovered that HbO exists in phospho-bound state Mtb remains associated with cell membrane Deoxy-HbO carries an autokinase activity...
Abstract Bardet-Biedl syndrome (BBS) is an incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into BBSome, a master regulator of ciliary membrane proteome. We report electron cryomicroscopy (cryo-EM) structures native bovine BBSome in inactive and active states at 3.1 3.5 Å resolution, respectively. In state, bound Arf-family GTPase (ARL6/BBS3) that recruits membranes. ARL6 recognizes...
Circular RNAs (circRNAs) are a novel family of endogenous and has become focus hot topic in biological research recent years. Emerging evidence revealed that circRNAs exert functions by acting as transcriptional regulators, microRNA sponges binding partners with RNA proteins. However, few researches have identified translational role potentially open hidden repertoire In this study, we unexpectedly discovered protein-encoding circular CCDC719-15 (circCCDC719-15) when were searching for...
Abstract Several ‘super-complexes’ of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer harvesting light energy, have been previously characterized in the mitochondrial cristae chloroplast thylakoid membranes. The latter reported here also be location an super-complex which dominated by ATP-synthase photosystem I (PSI) reaction-center defined mass spectrometry, clear-native PAGE Western Blot analyses. This...