A5073478356- Hemoglobin structure and function
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Neonatal Health and Biochemistry
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Microbial Metabolic Engineering and Bioproduction
- Erythrocyte Function and Pathophysiology
- Catalysis and Hydrodesulfurization Studies
- Bacteriophages and microbial interactions
- Enzyme Catalysis and Immobilization
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Microbial bioremediation and biosurfactants
- Biofuel production and bioconversion
- Wastewater Treatment and Nitrogen Removal
- Enzyme Production and Characterization
- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide and Herbicide Environmental Studies
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Invertebrate Immune Response Mechanisms
- Protein Structure and Dynamics
Illinois Institute of Technology
2015-2025
The Ohio State University
2022
Yale University
1974-2003
Kyoto University
1998
Kraft Heinz (United States)
1994
Indiana University Bloomington
1984-1985
The activity of ribonuclease P on precursor tRNA substrates from Escherichia coli can be abolished by pretreatment this enzyme with micrococcal nuclease or pancreatic A, as well proteases and thermal denaturation. Highly purified RNase exhibits one prominent RNA polypeptide component when examined in polyacrylamide gels containing sodium dodecyl sulfate. buoyant density CsCl P, 1.71 g/ml, is characteristic a protein-RNA complex. inhibited various molecules. presence discrete appears to...
The obligate aerobic bacterium, Vitreoscilla, synthesizes elevated quantities of a homodimeric hemoglobin (VHb) under hypoxic growth conditions. Expression VHb in heterologous hosts often enhances and product formation. A role facilitating oxygen transfer to the respiratory membranes is one explanation its cellular function. Immunogold labeling both Vitreoscilla recombinant Escherichia coli bearing gene clearly indicated that has cytoplasmic (not periplasmic) localization concentrated near...
The bacterium, Vitreoscilla, can induce the synthesis of a homodimeric hemoglobin under hypoxic conditions. Expression VHb in heterologous bacteria often enhances growth and increases yields recombinant proteins production antibiotics, especially oxygen-limiting There is evidence that interacts with bacterial respiratory membranes cytochrome bo proteoliposomes. We have examined whether there are binding sites for on cytochrome, using yeast two-hybrid system as bait testing every Vitreoscilla...
Abstract The characteristics of growth and synthesis plasmid‐encoded protein were studied for strains recombinant E. coli JM103 which carried the β‐lactamase gene on plasmids different sizes. used included vector pUC8 its derivatives containing varying‐sized inserts Drosophila DNA (not expressed in ). Luria broth (LB) a minimal medium (M9) supplemented some cases with additional inorganic phosphate as media. There was no evidence segregational instability these experiments, where antibiotic...
RNase P can cleave in vitro a bacteriophage phi80-induced RNA which is 62 nucleotides long [M3 RNA, G. Pieczenik et al. (1972) Arch. Biochem. Biophys. 152, 152-165] to yield two specific fragments 25 and 37 long. As the case for another substrate of P; precursor Escherichia coli 4.5S cleavage site M3 at end double-stranded region immediately adjacent single-stranded segment. Similar nucleotide sequences span both substrates. These other features reaction with are different from some aspects...
In previous work from our laboratories a synthetic gene encoding peptide ("Sulpeptide 1" or "S1") with high proportion of methionine and cysteine residues had been designed to act as sulfur sink was inserted into the dsz (desulfurization) operon Rhodococcus erythropolis IGTS8. described here this construct (dszAS1BC) intact (dszABC) cloned vector pRESX under control (Rhodococcus) kstD promoter were transformed desulfurization-negative strain CW25 qingshengii. The resulting strains...
Abstract The gene ( vgb ) encoding the hemoglobin (VHb) of Vitreoscilla sp. was cloned into a broad host range vector and stably transformed Burkholderia (formerly Pseudomonas strain DNT, which is able to degrade metabolize 2,4‐dinitrotoluene (DNT). Vgb maintained expressed in functional form this recombinant (YV1). When growth YV1, both tryptic soy broth minimal salts containing DNT yeast extract, compared with that untransformed strain, YV1 grew significantly better on cell mass basis A...
Ethanol production from whey powder was investigated by using free as well alginate immobilized E. coli and expressing Vitreoscilla hemoglobin (VHb) in both shake flask fermenter cultures. Media with varying levels of (lactose contents 3%, 5%, 8% or 15%) yeast extract (0.3% 0.5%) were evaluated fermentation times 48–96 h. Immobilization VHb expression resulted higher ethanol all media; the increases ranged 2% to 89% for immobilization 182% expression. It determined that growth medium...
Xanthomonas maltophilia was transformed with the gene encoding Vitreoscilla (bacterial) hemoglobin, vgb, and growth of engineered strain compared that untransformed using benzoic acid as sole carbon source. In general, greater than strain; this true for experiments both overnight cultures log phase cells inocula, but particularly latter. cases also more efficient in converting into biomass.