A5064075143- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Electron and X-Ray Spectroscopy Techniques
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Vibrio bacteria research studies
- Advanced X-ray Imaging Techniques
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Mechanical Failure Analysis and Simulation
- Scientific Computing and Data Management
- Genetics, Bioinformatics, and Biomedical Research
- Biodiesel Production and Applications
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Microstructure and Mechanical Properties of Steels
- Advanced Proteomics Techniques and Applications
- Machine Learning in Materials Science
- AI in cancer detection
- Research Data Management Practices
- Metal Forming Simulation Techniques
- Algal biology and biofuel production
- Biofuel production and bioconversion
- Escherichia coli research studies
- Metallurgy and Material Forming
European Bioinformatics Institute
2018-2024
Imperial College London
2016-2018
Jiangsu University
2018
Birkbeck, University of London
2008
University College London
2008
The Protein Data Bank (PDB) is the single global archive of experimentally determined three-dimensional (3D) structure data biological macromolecules. Since 2003, PDB has been managed by Worldwide (wwPDB; wwpdb.org), an international consortium that collaboratively oversees deposition, validation, biocuration, and open access dissemination 3D macromolecular data. Core Archive houses atomic coordinates more than 144 000 structural models proteins, DNA/RNA, their complexes with metals small...
Abstract The Protein Data Bank in Europe (PDBe), a founding member of the Worldwide (wwPDB), actively participates deposition, curation, validation, archiving and dissemination macromolecular structure data. PDBe supports diverse research communities their use structures by enriching PDB data providing advanced tools services for effective access, visualization analysis. This paper details enrichment at PDBe, including mapping RNA to Rfam, identification molecules that act as cofactors. has...
Abstract Public archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org) catering for atomic models and Electron Microscopy (EMDB; emdb-empiar.org) 3D reconstructions from cryo-EM experiments. Even before recent rapid growth cryo-EM, there was an expressed community need a public archive of image data experiments validation, software development, testing training. Concomitantly, proliferation imaging techniques cells, tissues organisms using volume EM...
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for deposition and validation of cryoEM structures, with focus on single-particle analysis. The meeting attended by 45 experts in processing, model building refinement, validation, archiving such structures. This report describes the workshop's motivation history, topics discussed, consensus recommendations resulting from workshop. Some challenges future methods-development efforts this area are also...
Abstract The type VI secretion system (T6 SS ) is a supra‐molecular bacterial complex that resembles phage tails. It killing machine which fires toxins into target cells upon contraction of its Tss BC sheath. Here, we show TssA1 T6 component forming dodecameric ring structures whose dimensions match those the sheath and can accommodate inner Hcp tube. binds impacts behaviour in vivo . In phage, first disc gp18 sits on baseplate wherein gp6 ring. We found remarkable sequence structural...
Pseudomonas aeruginosa has three type VI secretion systems (T6SSs), H1-, H2-, and H3-T6SS, each belonging to a distinct group. The two T6SS components, TssB/VipA TssC/VipB, assemble form tubules that conserve structural/functional homology with tail sheaths of contractile bacteriophages pyocins. Here, we used cryoelectron microscopy solve the structure H1-T6SS P. TssB1C1 sheath at 3.3 Å resolution. Our allowed us resolve some features were not resolved in Vibrio cholerae VipAB Francisella...
Organised data is easy to use but the rapid developments in field of bioimaging, with improvements instrumentation, detectors, software and experimental techniques, have resulted an explosion volumes being generated, making well-organised elusive goal. This guide offers a handful recommendations for bioimage depositors, analysts microscope developers, whose implementation would contribute towards better organised preparation archival. Based on our experience archiving large image datasets...
Abstract Public archiving in structural biology is well established with the Protein Data Bank (PDB; wwPDB.org ) catering for atomic models and Electron Microscopy (EMDB; emdb-empiar.org 3D reconstructions from cryo-EM experiments. Even before recent rapid growth cryo-EM, there was an expressed community need a public archive of image data experiments validation, software development, testing training. Concomitantly, proliferation imaging techniques cells, tissues organisms using volume EM...
<ns3:p>Organised data is easy to use but the growth of bioimaging, with improvements in instrumentation, detectors, software and experimental techniques has resulted an explosion volumes being generated, making this elusive goal. This guide offers a handful recommendations whose implementation would contribute towards better organised preparation for archival. Based on our experience archiving large image datasets EMPIAR, BioImage Archive BioStudies, we propose number strategies that believe...