A5073357045- Metalloenzymes and iron-sulfur proteins
- Advanced Electron Microscopy Techniques and Applications
- Metal complexes synthesis and properties
- Photoreceptor and optogenetics research
- Organometallic Complex Synthesis and Catalysis
- Photosynthetic Processes and Mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Asymmetric Hydrogenation and Catalysis
- Neuroscience and Neuropharmacology Research
- Crystallography and molecular interactions
- Metal-Catalyzed Oxygenation Mechanisms
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Chemical Reaction Mechanisms
- Inorganic Chemistry and Materials
- Organometallic Compounds Synthesis and Characterization
- Mass Spectrometry Techniques and Applications
- Electrocatalysts for Energy Conversion
- Ammonia Synthesis and Nitrogen Reduction
- Magnetism in coordination complexes
- CCD and CMOS Imaging Sensors
- Spectroscopy and Quantum Chemical Studies
- Protein Structure and Dynamics
MRC Laboratory of Molecular Biology
2016-2025
Skagit Valley Hospital
2005-2024
Medical Research Council
1985-2023
University of Cambridge
1988-2023
University of York
2021
Newcastle University
2007-2019
Covenant Medical Center
2019
Baylor School
2019
Wisconsin Department of Natural Resources
2017
Newcastle upon Tyne Hospital
2007-2015
Three-dimensional (3D) structure determination by single particle electron cryomicroscopy (cryoEM) involves the calculation of an initial 3D model, followed extensive iterative improvement orientation individual images and resulting map. Because there is much more noise than signal at high resolution in images, this creates possibility reinforcement map, which can give a false impression attained. The balance between final map its limiting depends on image processing procedure not easily...
This Meeting Review describes the proceedings and conclusions from inaugural meeting of Electron Microscopy Validation Task Force organized by Unified Data Resource for 3DEM (http://www.emdatabank.org) held at Rutgers University in New Brunswick, NJ on September 28 29, 2010. At workshop, a group scientists involved collecting electron microscopy data, using data to determine three-dimensional (3DEM) density maps, building molecular models into maps explored how assess models, other that are...
An attempt has been made to fit the amino acid sequence of bacteriorhodopsin three-dimensional density map molecule. First, seven segments were selected as being probable transmembrane alpha helices. Then each 5040 possible ways fitting these into regions helical in evaluated based on criteria connectivity nonhelical link regions, charge neutralization, and total scattering per helix. A single model that may be experimentally tested emerged most probable.
Low dose electron imaging applications such as cyro-microscopy are now benefitting from the improved performance and flexibility of recently introduced detectors in which electrons directly incident on backthinned CMOS sensors. There currently three commercially available this type: Direct Electron DE 20, FEI Falcon II Gatan K2 Summit. These have different characteristics so it is important to compare their properties carefully with a view optimising how each used. Results at 300 keV for...
There has been enormous progress during the last few years in determination of three-dimensional biological structures by single particle electron cryomicroscopy (cryoEM), allowing maps to be obtained with higher resolution and from fewer images than required previously. This is due principally introduction a new type direct detector that 2- 3-fold detective quantum efficiency available previously, improvement computational algorithms for image processing. In spite great strides have made,...