A5058966527- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Biochemical and Structural Characterization
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Fungal and yeast genetics research
- Lysosomal Storage Disorders Research
- ATP Synthase and ATPases Research
- Reproductive Health and Contraception
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Caveolin-1 and cellular processes
- Diet and metabolism studies
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- Biotin and Related Studies
- Metabolism and Genetic Disorders
- Retinal Development and Disorders
- Cholesterol and Lipid Metabolism
- Autophagy in Disease and Therapy
- Venous Thromboembolism Diagnosis and Management
- Blood Coagulation and Thrombosis Mechanisms
- Lipoproteins and Cardiovascular Health
University of Groningen
2013-2024
Biotechnology Institute
2020-2023
University of Exeter
2022
Shanghai Jiao Tong University
2022
Keio University
2022
Estación Experimental del Zaidín
2022
Sanford Burnham Prebys Medical Discovery Institute
2022
Western University
2022
National Centre for Biological Sciences
2022
Heidelberg University
2022
Cytosolic lipid droplets (LDs) are ubiquitous organelles in prokaryotes and eukaryotes that play a key role cellular organismal homeostasis. Triacylglycerols (TAGs) steryl esters, which stored LDs, typically mobilized growing cells or upon hormonal stimulation by LD-associated lipases ester hydrolases. Here we show the yeast Saccharomyces cerevisiae, LDs can also be turned over vacuoles/lysosomes process morphologically resembles microautophagy. A distinct set of proteins involved LD...
Atherosclerosis is a chronic inflammatory disease driven by hypercholesterolemia. During aging, T cells accumulate cholesterol, potentially affecting inflammation. However, the effect of cholesterol efflux pathways mediated ATP-binding cassette A1 and G1 (ABCA1/ABCG1) on cell-dependent age-related inflammation atherosclerosis remains poorly understood. In this study, we generate mice with cell-specific Abca1/Abcg1-deficiency low-density-lipoprotein-receptor deficient (Ldlr-/-) background....
The bacterial plasma membrane is an important cellular compartment. In recent years it has become obvious that protein complexes and lipids are not uniformly distributed within membranes. Current hypotheses suggest flotillin proteins required for the formation of including cell-wall synthetic proteins. We show here flotillins factors fluidity homeostasis. Loss leads to a decrease in turn alterations MreB dynamics and, as consequence, peptidoglycan synthesis. These reverted when restored by...
Invaginations of the mitochondrial inner membrane, termed cristae, are hubs for oxidative phosphorylation. The contact site and cristae organizing system (MICOS) dimeric F1Fo-ATP synthase play important roles in controlling architecture. A fraction MICOS core subunit Mic10 is found association with ATP synthase, yet it unknown whether this interaction relevance or cellular functions. Here, we established conditions to selectively study role at synthase. variants impaired functions stimulate...
Using electron and fluorescence microscopy techniques, we identified various physical contacts between peroxisomes other cell organelles in the yeast Hansenula polymorpha. In exponential glucose-grown cells, which typically contain a single small peroxisome, were only observed with endoplasmic reticulum plasma membrane. Here focus on novel peroxisome-vacuole contact site that is formed when cells are shifted to methanol containing media, conditions induce strong peroxisome development. At...
ABSTRACT Here, we used fluorescence microscopy and a peroxisome-targeted tandem fluorescent protein timer to determine the relative age of peroxisomes in yeast. Our data indicate that yeast cells contain heterogeneous population relatively old young peroxisomes. During budding, peroxisome retention factor inheritance 1 (Inp1) selectively associates older organelles, which are retained mother cells. Inp2, required for transport bud, preferentially younger organelles. Using microfluidics...
We demonstrated that in the yeast Hansenula polymorpha peroxisome fission and degradation are coupled processes important to remove intra-organellar protein aggregates. Protein aggregates were formed peroxisomes upon synthesis of a mutant catalase variant. showed introduction these peroxisomal lumen had physiological disadvantages as it affected growth caused enhanced levels reactive oxygen species. Formation was followed by asymmetric separate aggregate from mother organelle. Subsequently,...
In the yeast Hansenula polymorpha peroxisomal membrane protein Pex11 and three endoplasmic reticulum localized proteins of Pex23 family (Pex23, Pex24 Pex32) are involved in formation peroxisome-ER contact sites. Previous studies suggested that these contacts non-vesicular lipid transfer important for expansion membrane. The absence Pex32 results a severe phenotype, while cells lacking Pex11, or show milder defects still capable to form peroxisomes grow on methanol. We performed transposon...
Pex1 and Pex6 are two AAA-ATPases that play a crucial role in peroxisome biogenesis. We have characterized the ultrastructure of Saccharomyces cerevisiae peroxisome-deficient mutants pex1 pex6 by various high-resolution electron microscopy techniques. observed cells contained peroxisomal membrane remnants, which ultrathin cross sections generally appeared as double rings. Electron tomography revealed these structures consisted one continuous membrane, representing an empty, flattened...
The yeast Hansenula polymorpha contains four members of the Pex23 family peroxins, which characteristically contain a DysF domain. Here we show that all H. proteins localize to endoplasmic reticulum (ER). Pex24 and Pex32, but not Pex29, predominantly accumulate at peroxisome-ER contacts. Upon deletion PEX24 or PEX32 - much lesser extent, PEX23 PEX29 contacts are lost, concomitant with defects in peroxisomal matrix protein import, membrane growth, organelle proliferation, positioning...
The SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin-5 (Stx5) is essential for Golgi transport. In humans, the STX5 mRNA encodes two isoforms, Stx5 Long (Stx5L) from first starting methionine and Short (Stx5S) an alternative at position 55. this study, we identify a human disorder caused by single missense substitution in second (p.M55V), resulting complete loss of short isoform. Patients suffer early fatal multisystem disease, including severe liver...
To mount an adaptive immune response, dendritic cells must migrate to lymph nodes present antigens T cells. Critical 3D migration is the nucleus, which size-limiting barrier for through extracellular matrix. Here, we show that inflammatory activation of leads nucleus becoming spherically deformed and enables overcome typical 2- 3-μm diameter limit gaps in We nuclear shape change partially attained reduced cell adhesion, whereas improved achieved reprogramming actin cytoskeleton....
We have analyzed the role of three members Pex11 protein family in peroxisome formation filamentous fungus Penicillium chrysogenum. Two these, and Pex11C, are components peroxisomal membrane, while Pex11B is present at endoplasmic reticulum. show that a major factor involved proliferation. also demonstrate P. chrysogenum cells deleted for known fission factors (all proteins Vps1) still contain peroxisomes. Interestingly, we find that, unlike mammals, Pex16 not essential biogenesis...
Retention of peroxisomes in yeast mother cells requires Inp1, which is recruited to the organelle by peroxisomal membrane protein Pex3. Here we show that Hansenula polymorpha Inp1 associates plasma membrane. Peroxisome-plasma contact sites disappear upon deletion INP1 but increase overexpression. Analysis truncated variants showed C terminus important for association peroxisome, while a stretch conserved positive charges and central pleckstrin homology-like domain are binding. In PEX3...
Here, we describe a novel peroxin, Pex37, in the yeast Hansenula polymorpha . H. Pex37 is peroxisomal membrane protein, which belongs to protein family that includes, among others, Neurospora crassa Woronin body Wsc, human PXMP 2, Saccharomyces cerevisiae mitochondrial inner Sym1, and its mammalian homologue MPV 17. We show deletion of PEX 37 does not appear have significant effect on peroxisome biogenesis or proliferation cells grown at peroxisome‐inducing growth conditions (methanol)....
Free radical generation plays a key role in many biological processes including cell communication, maturation, and aging. In addition, free is usually elevated cells under stress as the case for different pathological conditions. liver tissue, produce radicals when exposed to toxic substances but also, instance, cancer, alcoholic disease cirrhosis. However, are small, short-lived, occur low abundance making them challenging detect especially time resolve, leading lack of nanoscale...
Endosomal Sorting Complex Required for Transport (ESCRT) proteins can be transiently recruited to the plasma membrane repair and formation of extracellular vesicles. Here, we discovered micrometer-sized worm-shaped ESCRT structures that stably persist multiple hours at macrophages, dendritic cells, fibroblasts. These surround clusters integrins known cargoes The are tightly connected cellular support left behind by cells together with surrounding patches membrane. phospholipid composition is...
Aim of this study was to examine the effects a semi-solid and liquid carbohydrate feeding on exercise performance. Thirty-two male triathletes performed three trials (3 hours maximal at 75 % VO2max) with either (S), an iso-caloric (F) or placebo (P). Exercise consisted cycling (bout 1 3) running 2 4). Survival analysis revealed that median maximum test time (MTT) F, S P 180, 126 120 min, respectively. Median MTT longer F than (sign-test; p<0.05) (p< 0.001), no difference between P. Mean...
Saccharomyces cerevisiae Aat2p contains a peroxisomal targeting signal type-1 and localizes to peroxisomes in oleate-grown cells, but not glucose-grown cells. Here, we have investigated from the yeast Hansenula polymorpha, which lacks recognizable signal. tagged with GFP at its C terminus displays dual cytosol-peroxisome localization ethanol-grown The partial of persisted absence classical cycling receptors Pex5p Pex7p was reduced cells deleted for matrix protein import factors PEX1, PEX2...