A5026641866- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Diet and metabolism studies
- Lipid metabolism and disorders
- Metabolism, Diabetes, and Cancer
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Diabetes Treatment and Management
- Atherosclerosis and Cardiovascular Diseases
- Peroxisome Proliferator-Activated Receptors
- Lymphatic System and Diseases
- Lipid metabolism and biosynthesis
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Lipoproteins and Cardiovascular Health
- Genetic factors in colorectal cancer
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Digestive system and related health
- Neonatal Respiratory Health Research
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- Drug Transport and Resistance Mechanisms
- Biochemical Analysis and Sensing Techniques
- Epigenetics and DNA Methylation
University of Pittsburgh
2019-2024
University of Connecticut
2015-2019
University of Cincinnati
2010-2016
University of Cincinnati Medical Center
2010-2013
West Virginia University
2009-2012
Morgantown High School
2009
Sanford Burnham Prebys Medical Discovery Institute
2009
Institute for Medical Research
2009
<b>Background:</b> The hormone FGF21 is a potent regulator of carbohydrate and lipid metabolism promising drug for treating metabolic syndrome. <b>Results:</b> Farnesoid X receptor (FXR) agonists FGF19 induce hepatic secretion via transcriptional mechanism posttranscriptional mechanism, respectively. <b>Conclusion:</b> Activation the FXR pathway stimulates expression secretion. <b>Significance:</b> potential approach to enhance endogenous production reverse
Apolipoprotein A-IV (apoA-IV) is secreted by the small intestine in response to fat absorption. Here we demonstrate a potential role for apoA-IV regulating glucose homeostasis. ApoA-IV–treated isolated pancreatic islets had enhanced insulin secretion under conditions of high but not low glucose, suggesting direct effect enhance glucose-stimulated release. This enhancement involves cAMP at level distal Ca 2+ influx into β cells. Knockout results compromised and impaired tolerance compared...
Atherosclerosis is a chronic inflammatory disease driven by hypercholesterolemia. During aging, T cells accumulate cholesterol, potentially affecting inflammation. However, the effect of cholesterol efflux pathways mediated ATP-binding cassette A1 and G1 (ABCA1/ABCG1) on cell-dependent age-related inflammation atherosclerosis remains poorly understood. In this study, we generate mice with cell-specific Abca1/Abcg1-deficiency low-density-lipoprotein-receptor deficient (Ldlr-/-) background....
Calcific aortic valve disease (CAVD) occurs when subpopulations of cells undergo specific differentiation pathways, promoting tissue fibrosis and calcification. Lipoprotein particles carry oxidized lipids that promote valvular disease, but low-density lipoprotein–lowering therapies have failed in clinical trials, there are currently no pharmacological interventions available for this disease. Apolipoproteins known promoters atherosclerosis, whether they possess pathogenic properties CAVD is...
Dietary fat is an important mediator of atherosclerosis and obesity. Despite its importance in mediating metabolic disease, there still much unknown about dietary absorption the intestine especially detailed biological roles intestinal apolipoproteins involved that process. We were specifically interested determining physiological role apolipoprotein A-IV (A-IV) using knockout (KO) mice. stimulated by secreted on nascent chylomicrons into lymph. found KO mice had reduced plasma triglyceride...
Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, acute pump response in mesentery to a postprandial meal has gone unexplored. In this study, we used rat mesenteric collecting vessel as an vivo model quantify effect of lipoproteins on Lipid load was continuously monitored by using intensity fluorescent fatty-acid analog, which infused along with fat emulsion duodenal cannula. The...
Since its initial report in 2009, the intestinal enteroid culture system has been a powerful tool used to study stem cell biology and development gastrointestinal tract. However, major question is whether enteroids retain function physiology. There have significant contributions describing ion transport physiology of human organoid cultures, as well gastric organoids, but critical studies on dietary fat absorption chylomicron synthesis primary not undertaken. Here we that murine cultures...
Expression of G6PD is controlled by changes in the degree splicing mRNA response to nutrients diet. This regulation involves an exonic enhancer (ESE) exon 12 mRNA. Using model, we demonstrate that and hormones control activity serine-arginine-rich (SR) proteins, a family co-activators, thereby regulate In primary rat hepatocyte cultures, insulin increased amount phosphorylated SR this effect was counteracted arachidonic acid. The results RNA affinity analysis with nuclear extracts from...
Abstract Apolipoprotein C-III (apoC-III) is not only predominantly synthesized by the liver but also small intestine. Because apoC-III secreted from intestine on chylomicron along with lipid absorption, we questioned whether might play a role in intestinal absorption and/or transport. Using both wild-type (WT) and transgenic (apoC-III Tg) mice, showed that Tg mice have decreased lymphatic transport compared WT response to an intraduodenal infusion of radiolabeled lipid. This associated...
The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), enhance postprandial insulin secretion, promote adipogenesis, regulate gastrointestinal motility food intake. To date, a consensus on how the response is altered in obesity lacking. We investigated effects of chronic high-fat (HF) feeding secretion lymph fistula rat model. Male Sprague-Dawley rats (8 wk) were provided semipurified AIN93M HF or low-fat (LF) diet ad libitum for 3 13...
Chylomicron metabolism is critical for determining plasma levels of triacylglycerols (TAGs) and cholesterol, both which are risk factors CVD. The rates chylomicron secretion remnant clearance controlled by intracellular extracellular factors, including apoC-III. We have previously shown that human apoC-III overexpression in mice (
Apolipoprotein A-IV (apoA-IV) is synthesized by the intestine and secreted when dietary fat absorbed transported into lymph associated with chylomicrons. We have recently demonstrated that loss of apoA-IV increases chylomicron size delays its clearance from blood. There still uncertainty, however, about precise role on transport lymph. ApoA-IV knockout (KO) mice do not a gross defect in lipid absorption, as measured oral tolerance fecal measurements. Here, using vivo fistula mouse model, we...
Lipid absorption begins with the digestion of dietary triacylglycerol and ultimately results in secretion chylomicrons into lymphatics. Additionally, intestine also secretes numerous proteins peptides involved lipid lipoprotein metabolism response to food. Ultimately, these proteins, peptides, hormones are found lymph. The lymph fistula rat model has traditionally been used study this intestinal nutrients, especially lipids, but recently, for studying by small intestine. protocols described...