A5007118437- 3D Printing in Biomedical Research
- Lymphatic System and Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Parasitic Diseases Research and Treatment
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Fetal and Pediatric Neurological Disorders
- Electrowetting and Microfluidic Technologies
- Advanced Drug Delivery Systems
- Pancreatic and Hepatic Oncology Research
- Advanced Chemical Sensor Technologies
- Engineering Education and Technology
- Parasites and Host Interactions
- Academic Writing and Publishing
- scientometrics and bibliometrics research
- Planarian Biology and Electrostimulation
- Prenatal Screening and Diagnostics
- Academic Publishing and Open Access
- Nonmelanoma Skin Cancer Studies
- Lymphatic Disorders and Treatments
- Interdisciplinary Research and Collaboration
- Mosquito-borne diseases and control
- Analytical Chemistry and Sensors
Massachusetts Institute of Technology
2016-2024
ResearchWorks (United States)
2024
Georgia Institute of Technology
2012-2018
Cambridge Electronics (United States)
2018
IIT@MIT
2017-2018
Hôpital Necker-Enfants Malades
2012-2014
Université Paris Cité
2014
Parker Hannifin (United States)
2012
l'Assurance Maladie
2012
Société Française d'Allergologie
2012
Microphysiological systems (MPSs) are in vitro models that capture facets of vivo organ function through use specialized culture microenvironments, including 3D matrices and microperfusion. Here, we report an approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-system microfluidic platforms compatible with the quantitative study a range compounds, lipophilic drugs. We describe three platform designs - "4-way", "7-way", "10-way" each accommodating...
A reported 96,480 people were diagnosed with melanoma in the United States 2019, leading to 7230 deaths. Early-stage identification of suspicious pigmented lesions (SPLs) primary care settings can lead improved prognosis and a possible 20-fold reduction treatment cost. Despite this clinical economic value, efficient tools for SPL detection are mostly absent. To bridge gap, we developed an analysis system wide-field images using deep convolutional neural networks (DCNNs) applied it 38,283...
Abstract Organoid cultures are proving to be powerful in vitro models that closely mimic the cellular constituents of their native tissue. Organoids typically expanded and cultured a 3D environment using either naturally derived or synthetic extracellular matrices. Assessing morphology growth characteristics these has been difficult due many imaging artifacts accompany corresponding images. Unlike single cell cultures, there no reliable automated segmentation techniques allow for...
Near-infrared imaging of lymphatic drainage injected indocyanine green (ICG) has emerged as a new technology for clinical architecture and quantification vessel function, yet the capabilities this approach have to be quantitatively characterized. We seek quantify its diagnostic tool disease. Imaging is performed in tissue phantom sensitivity analysis hairless rats vivo testing. To demonstrate efficacy quantifying immediate functional changes lymphatics, we investigate effects topically...
Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, acute pump response in mesentery to a postprandial meal has gone unexplored. In this study, we used rat mesenteric collecting vessel as an vivo model quantify effect of lipoproteins on Lipid load was continuously monitored by using intensity fluorescent fatty-acid analog, which infused along with fat emulsion duodenal cannula. The...
The intestinal mucosal barrier forms a critical interface between lumen contents such as bacteria, drugs, and drug carriers the underlying tissue. Current in vitro models, while recapitulating certain...
Nearly all dietary lipids are transported from the intestine to venous circulation through lymphatic system, yet mechanisms that regulate this process remain unclear. Elucidating involved in functional response of lymphatics changes lipid load would provide valuable insight into recent implications dysfunction related diseases. Therefore, we sought develop an situ imaging system quantify and correlate function as it relates transport. The platform provides capability dual-channel both...
The ability to quantify collecting vessel function in a minimally invasive fashion is crucial the study of lymphatic physiology and role pump disease progression. Therefore, we developed highly sensitive, research platform for quantifying pumping capacity vessels rodent tail forelimb. To achieve this, have integrated near-infrared imaging system with feedback-controlled pressure cuff modulate lymph flow. After occluding flow by inflating on limb or tail, gradually deflate while restoration...
Abstract In Fall 2016, the MIT Dean of Engineering chartered New Education Transformation (NEET) initiative, a new cross-departmental effort to rethink engineering education (what students learn and how learn) in fundamental way across school engineering. NEET aims educate young engineers build “new machines systems” that will address societal challenges 21st century. alumnus be prepared work as entrepreneurs/innovators, makers discoverers, future leaders through learning practicing Ways...
Precise fluid height sensing in open-channel microfluidics has long been a desirable feature for wide range of applications.
Authorship of peer-reviewed journal articles and abstracts has become the primary currency reward unit in academia. Such a is crucial for students postdocs who are often under-compensated thus highly value authorship as an incentive. While numerous scientific publishing organizations have written guidelines determining author qualifications order, there remains much ambiguity when it comes to how these criteria weighed by research faculty. Here, we sought provide some initial insight on...
Abstract The New Engineering Education Transformation (NEET) program is an elective three-year undergraduate interdisciplinary at Massachusetts Institute of Technology. pivoted from in-person on-campus teaching to remote during Spring 2020 due the COVID-19 pandemic. NEET has five cross-departmental tracks, or threads, across science, technology, engineering, and mathematics disciplines. It completed its third year operation graduated founding cohort in grown become fifth-largest academic...
With the rise of research utilizing microphysiological systems (MPSs), need for tools that enable physiological mimicking relevant cellular environment is vital. The limited ability to reproduce crucial features microenvironment, such as surrounding fluid flow and dynamic changes in biochemical stimuli, severely limits types experiments can be carried out. Current equipment achieve this, syringe peristaltic pumps, expensive, large, difficult program has potential scalability. Here, we...
A synthetic polymer nanoparticle formulation utilizing the physiological nitrosothiol chemistry for nitric oxide delivery is shown. Toxicity of S-nitroso nanoparticles against adult female Brugia malayi worms, which are responsible lymphatic filariasis, dependent on release through transnitrosation as S-nitrosocysteine, a potent endogenous donor.
We present LSM1-MS2, a pre-trained self-supervised foundation model designed for tandem mass spectrometry (MS/MS) utilizing transformer architecture with custom tokenization masked MS2 peak reconstruction. Our is fine-tuned on smaller, labeled datasets tasks such as compound property prediction, spectral matching, and de novo molecular generation. LSM1-MS2 demonstrates superior performance compared to traditional supervised models, achieving high accuracy minimal data. It outperforms...
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting vessels and lymph nodes for years, often resulting lymphedema, elephantiasis or hydrocele. mechanisms driving worm migration retention within lymphatics are currently unknown. We have developed an integrated vitro imaging platform capable of quantifying malayi behavior...
Abstract With the rise of research utilizing microphysiological systems (MPSs), need for tools that enable physiological mimicking relevant cellular environment is vital. The limited ability to reproduce crucial features microenvironment, such as surrounding fluid flow and dynamic changes in biochemical stimuli, severely limits types experiments can be carried out. Current equipment achieve this, syringe peristaltic pumps, expensive, large, difficult program has potential scalability. Here,...
Abstract Lymphatic filariasis is a neglected tropical disease caused by roundworm parasites such as Brugia malayi that spread via mosquito vector. In vitro culture of these provides controlled conditions to understand parasite biology and cheaper way screen potential micro- macrofilaricides. Published studies have used wide array approaches metrics regarding in cultures B. ; result, drawing comparisons identifying the reasons why inability reproduce outcomes are difficult. This study sought...
Abstract Epithelial organoids are now an important tool in fields ranging from regenerative medicine to drug discovery. Organoid culture requires Matrigel, a complex, tumor-derived, extracellular matrix. An alternative completely synthetic matrix could improve reproducibility, clarify mechanistic phenomena, and enable applications involving human implantation. Here, we designed matrices with tunable biomolecular biophysical properties that allowed us identify critical gel parameters organoid...
Abstract Precise fluid height sensing in open-channel microfluidics has long been a desirable feature for wide range of applications. However, performing accurate measurements the level small-scale reservoirs (<1mL) proven to be an elusive goal, especially if direct fluid-sensor contact needs avoided. In particular, gravity-driven systems used several microfluidic applications establish pressure gradients and impose flow remain open-loop largely unmonitored due these limitations. Here we...
We introduce LSM1-MS2, a self-supervised foundation model pre-trained on extensive unlabeled MS2 data for tandem mass spectrometry. LSM1-MS2 leverages the complex within spectra, bypassing need detailed identification and efficiently processing traditional data. The model's architecture, based specialized transformer custom tokenization scheme, enables Masked Peak Modeling, which trains to reconstruct peaks, preparing it various downstream applications. Fine-tuning with smaller, labeled...
We introduce LSM1-MS2, a self-supervised foundation model pre-trained on extensive unlabeled MS2 data for tandem mass spectrometry. LSM1-MS2 leverages the complex within spectra, bypassing need detailed identification and efficiently processing traditional data. The model's architecture, based specialized transformer custom tokenization scheme, enables Masked Peak Modeling, which trains to reconstruct peaks, preparing it various downstream applications. Fine-tuning with smaller, labeled...
Mass spectrometry-based metabolomics allows for the quantitation of metabolite levels in diverse biological samples. The traditional method converting peak areas to absolute concentrations involves use matched heavy isotopologues. However, this approach is laborious and limited a small number metabolites. We addressed these limitations by developing PyxisTM, machine learning-based technology which converts raw mass spectrometry data concentration measurements without need per-analyte...
Abstract Mucus layers, viscoelastic gels abundant in anionic mucin glycoproteins, obstruct therapeutic delivery across all mucosal surfaces. We found that strongly positively charged nanoparticles (NPs) rapidly adsorb a protein corona mucus, impeding cell binding and uptake. To overcome this, we developed mucus-evading, cell-adhesive (MECS) NPs with variable surface charge using Flash NanoPrecipitation, by blending neutral poly(ethylene glycol) (PEG) for mucus transport small amount, 5 wt%,...