A5101665543- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Mass Spectrometry Techniques and Applications
- Diet and metabolism studies
- Mast cells and histamine
- Diabetes Treatment and Management
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Cellular transport and secretion
- Phytochemistry and Biological Activities
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Polyamine Metabolism and Applications
- Kruppel-like factors research
- Gut microbiota and health
- Pancreatic function and diabetes
- Analytical chemistry methods development
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Fibroblast Growth Factor Research
- Liver Disease Diagnosis and Treatment
- Neuropeptides and Animal Physiology
- Trace Elements in Health
- Endoplasmic Reticulum Stress and Disease
Nanjing Medical University
2024
Jinan University
2024
Tropical Crops Genetic Resources Institute
2017-2023
China Agricultural University
2016-2023
Chinese Academy of Tropical Agricultural Sciences
2017-2023
University of Birmingham
2023
Southern University of Science and Technology
2023
Ministry of Agriculture and Rural Affairs
2023
University of Cincinnati
2004-2022
Affiliated Hospital of Jining Medical University
2022
Fibroblast growth factor 21 (FGF21) has been identified as a potent metabolic regulator. Administration of recombinant FGF21 protein to rodents and rhesus monkeys with diet-induced or genetic obesity diabetes exerts strong antihyperglycemic triglyceride-lowering effects reduction body weight. Despite the importance in regulation glucose, lipid, energy homeostasis, mechanisms by which functions regulator remain largely unknown. Here we demonstrate that regulates homeostasis adipocytes through...
Glucagon-like peptide 1 (GLP-1) is a gastrointestinal hormone secreted in response to meal ingestion by enteroendocrine L cells located predominantly the lower small intestine and large intestine. GLP-1 inhibits secretion motility of upper gut has been suggested play role "ileal brake." In this study, we investigated effect recombinant GLP-1-(7-36) amide (rGLP-1) on lipid absorption intestinal lymph duct-cannulated rats. addition, effects rGLP-1 production apolipoprotein (apo) B apo A-IV,...
Chlorinated hydrocarbons are lipophilic, toxic, and persistent in the environment animal tissues. They enter body food stored adipose tissue. Loss of fat through caloric restriction mobilizes lipophilic xenobiotics results distribution to other We have studied reversibility this process mice that followed a regimen weight cycling. Weight gain was by loss, second gain, loss ("yo-yo diet regimen"). measured orally gavaged [14C]hexachlorobenzene, which is sparingly metabolized. found cycling...
After the ingestion of nutrients, secretion incretin hormones glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) by enteroendocrine cells increases rapidly. Previous studies have shown that oral fat stimulates both incretins; however, it is unclear whether there a dose-dependent relationship between amount lipid ingested in vivo. Recently, we found higher concentration intestinal lymph than peripheral or portal plasma. We therefore used fistula rat model...
Cardiovascular complications are the major causes of mortality and morbidities in diabetic patients. The patients have an increased risk developing atherosclerosis, which could lead to heart attack stroke. Glucagon-like peptide 1 (GLP-1) receptor agonists a class potent anti-glycemic agents treat diabetes. Recently, several GLP-1 been found cardiovascular benefit independent their glucose lowing ability. Liraglutide is one clinically approved effective agonists. In this study, we explored...
Ambient pressure ion mobility time-of-flight mass spectrometry (IMMS) has recently emerged as a rapid and efficient analytical technique for applications to metabolomics. An important application of metabolomics is monitor metabolome shifts caused by stress due toxin exposure, nutritional changes, or disease. The research presented in this paper uses IMMS metabolic changes rat lymph fluid dietary stresses over time. Extracts metabolites found the collected from stressed rats were subjected...
Abstract Knowledge of regulation glucose transport contributes to our understanding whole-body homoeostasis and human metabolic diseases. Isoleucine has been reported participate in levels many studies; therefore, this study was designed examine the effect isoleucine on intestinal muscular GLUT expressions. In an animal experiment, were determined weaning pigs fed control or isoleucine-supplemented diets. Supplementation diet significantly increased piglet average daily gain, enhanced GLUT1...
Objective To investigate the cellular mechanisms of action tanshinone IIA on fatty liver disease induced by a high-fat diet in an animal model non-alcoholic (NAFLD). Methods Adult male Sprague Dawley rats were randomized into one three groups: regular rat (CON group) for 4 months; (HFD HFD 2 months followed treatment plus (TAN further months. A range physical and biochemical markers lipid accumulation measured compared between groups. Results Tanshinone significantly reduced fat plasma...
Previous studies have linked certain types of gut mucosal immune cells with fat intake. We determined whether absorption activates intestinal mast (MMC), a key component the system. Conscious lymph fistula rats were used. The mesenteric ducts cannulated, and intraduodenal (i.d.) tubes installed for infusion Liposyn II 20% (an intralipid emulsion). Lymphatic concentrations histamine, rat MMC protease (RMCPII), specific marker degranulation, prostaglandin D(2) (PGD(2)) measured by ELISA....
ZnT8 is a zinc transporter enriched in pancreatic β-cells, and its polymorphism associated with increased susceptibility to type 2 diabetes. However, the exact role of systemic energy metabolism remains elusive. In this study, we found that knockout mice displayed adiposity without obvious weight gain. We also observed intestinal tract morphology, motility, gut microbiota were changed mice. Further study demonstrated was expressed enteroendocrine cells, especially 5-hydroxytryptamine...
Neuregulin 4 (Nrg4), an adipose tissue–enriched endocrine factor, participates in adipocyte-to-hepatocyte communication, eliciting beneficial metabolic effects nonalcoholic fatty liver disease (NAFLD). We evaluate the physiological roles of NRG4 humans and unravel role variants pathogenesis NAFLD related disorders. identified two rare missense mutations—p.R44H p.E47Q—in EGF-like domain by whole-exome sequencing 224 severely obese subjects exome genotyping 2,388 from Shanghai Obesity Study....
Diamine oxidase (DAO) is abundantly expressed in mammalian small intestine catalyzing the oxidative breakdown of polyamines and histamine. The aim this study was to determine relationship between stimulation intestinal diamine secretion with fat absorption histamine release. Conscious lymph fistula rats were used. mesenteric ducts cannulated intraduodenal tubes installed for infusion Liposyn II 20% (an intralipid emulsion). Lymphatic DAO activity protein analyzed by radiometric assay Western...
Regulated secretion is an intracellular pathway that highly conserved from protists to humans. Granin family proteins were proposed participate in the biogenesis, maturation and release of secretory granules this pathway. However, exact molecular mechanisms underlying functions granin remain unclear. Here, we show chromogranin B (CHGB), a granule protein, inserts itself into membrane forms chloride-conducting channel. CHGB interacts strongly with phospholipid membranes through two...
Rationale Traditionally, drug analysis in biological tissue by mass spectrometry has required complicated sample pre‐treatment, which not only wasted time, but also had adverse effects on the results. In order to assist assessment of potential drugs rapidly and accurately, a direct analytical method for detection tissues is needed. The development such described this study. Methods An anti‐cancer drug, 9‐phenylacridine (ACPH), injected into kidney mice, was directly analysed from placed...
It is not well understood how monosodium glutamate (MSG) affects gastrointestinal physiology, especially regarding the absorption and subsequent transport of dietary lipids into lymph. Thus far, there little information about ingestion MSG lipid lipolysis, uptake, intracellular esterification, formation secretion chylomicrons. Using lymph fistula rats treated with infusion a 2% solution before continuous triglyceride, we show that causes significant decrease in both triglyceride cholesterol...